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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GFP Antibody LS‑C121913

Catalog Number Size Price
LS-C121913-100 100 µl $875 
Antibody

GFP Antibody LS‑C121913

Antibody:
Mouse Monoclonal to Aequorea victoria GFP
Application:
IHC, IHC-P, IHC-Fr, WB, ELISA
Reactivity:
Aequorea victoria
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$875 
LS-C121913-100
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Aequorea victoria GFP
Application:
IHC, IHC-P, IHC-Fr, WB, ELISA
Reactivity:
Aequorea victoria
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
GFP antibody LS-C121913 is an unconjugated mouse monoclonal antibody to aequorea victoria GFP. Validated for ELISA, IHC and WB.
Target
Aequorea victoria GFP
Host
Mouse
Reactivity
Aequorea victoria (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, Biotin, FITC, HRP, PE.
Purification
Purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:500 - 1:1000)
  • IHC - Frozen (1:500 - 1:1000)
  • Western blot (1:2000 - 1:5000)
  • ELISA (1:1000 - 1:2000)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Bacterially expressed GFP.
Specificity
Recognizes Green Fluorescent Protein (GFP).
Usage
Suitable for use in ELISA, Immunohistochemistry (frozen and paraffin), and Western Blot. Immunohistochemistry (frozen): 1:500-1:1000. Immunohistochemistry (paraffin):1:500-1:1000. ELISA: 1:1000-1:2000. Western Blot: 1:2000-1:5000.
Presentation
PBS, BSA
Storage
May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for up to 1 year at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GFP
P42212 M62653 P42212

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Anti-GFP Chemiluminescent Kit for Western blot. Known amounts of recombinant GFP protein ( were spiked into a HeLa cell-derived lysates (p/n W09-000-364) and separated by SDS-PAGE using a 4-20% gradient gel. Proteins were transferred onto a nitrocellulose membrane for 1 h at 100 mV. The membrane was blocked with TTBS (p/n MB-013) supplemented with 1% BSA (p/n BSA-50) for 1 h at 4? prior to probing the blot with the anti-GFP monoclonal antibody Anti-GFP (MOUSE) Monoclonal Antibody - 600-301-215 diluted 1:1000 for 40 min. Detection of the primary antibody by the HRP-conjugated anti-Mouse IgG (p/n LS-C60772) was performed at a dilution of 1:20000 for 1h at 4?. FemtoMax Super Sensitive Chemiluminescent Luminol Substrate was used for signal detection (see below).
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Requested From: United States
Date Requested: 9/15/2019