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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C50850-500 500 µg (1 mg/ml) $530 
Antibody

Polyclonal Goat anti‑Aequorea victoria GFP Antibody (HRP, IHC, IF, WB) LS‑C50850

Polyclonal Goat anti‑Aequorea victoria GFP Antibody (HRP, IHC, IF, WB) LS‑C50850

Antibody:
Goat Polyclonal to Aequorea victoria GFP
Application:
IHC, IF, WB, ELISA
Reactivity:
Aequorea victoria
Format:
Horseradish Peroxidase, Unmodified
Price
Catalog Number
$530 
LS-C50850-500
500 µg
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Goat Polyclonal to Aequorea victoria GFP
Application:
IHC, IF, WB, ELISA
Reactivity:
Aequorea victoria
Format:
Horseradish Peroxidase, Unmodified

Specifications

Description
GFP antibody LS-C50850 is an HRP-conjugated goat polyclonal antibody to aequorea victoria GFP. Validated for ELISA, IF, IHC and WB. Cited in 1 publication.
Target
Aequorea victoria GFP
Synonyms
GFP
Host
Goat
Reactivity
Aequorea victoria (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Horseradish Peroxidase
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC (1:500 - 1:3000)
  • Immunofluorescence
  • Western blot (1:1000 - 1:5000)
  • ELISA (1:10000 - 1:50000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
GST-Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Specificity
Recognizes wild type, recombinant and enhanced GFP. Assay by IEP resulted in a single precipitin arc against anti-goat serum, anti-peroxidase and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against human, mouse and rat serum proteins.
Usage
Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. ELISA: 1:10000-1:50000. Sandwich or capture. Recognizes wild type, recombinant and enhanced forms of GFP. Immunohistochemistry: 1:500-1:3000. Immunofluorescence: Fluorochrome conjugated anti-GFP was assayed by immunofluorescence microscopy on prokaryotic. (E. coli) and eukaryotic (CHO cells) expression systems and was shown to detect GFP containing inserts. Significant amplification of signal was detected using fluorochrome conjugated anti-GFP relative to the fluorescence of GFP alone. Western Blot: 1:1000-1:5000. Detects a 42kD band. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
10 mg/ml BSA (IgG, Protease free), PBS, pH 7.2, 0.01% Gentamicin
Storage
May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for up to 1 year. Sodium azide should not be added to HRP conjugates. Protect from light.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GFP
P42212 M62653 P42212

LSBio Ratings

GFP Antibody (HRP) for IHC, IF/Immunofluorescence, WB/Western, ELISA LS-C50850 has an LSBio Rating of
Publications (4)

Publications (1)

Dystroglycan controls dendritic morphogenesis of hippocampal neurons in vitro. Bijata M, Wlodarczyk J, Figiel I. Frontiers in cellular neuroscience. 2015 9:199. (IHC; Aequorea victoria)

Customer Reviews (0)


Requested From: United States
Date Requested: 7/4/2020