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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
Protein Family And Group
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Catalog Number Size Price
LS-C154191-1 1 mg (1 mg/ml) $495 
GFP Antibody - Western Blot of Goat anti-GFP antibody Peroxidase conjugated. Marker: Opal Pre-stained ladder  Lane 2: HEK293 lysate  Lane 3: HeLa Lysate  Lane 4: CHO/K1 Lysate  Lane 5: C2C12 Lysate. Lane 6: NIH/3T3 Lysate  Lane 7: Mouse Embryonic Fibroblast Lysate  Lane 8: E-coli HCP Control  Lane 9: 2 Epitope MBP Tag Marker Lysate  Lane 10: Recombinant Red Fluorescent Protein  Lane 11: Green Fluorescent Protein  Lane 12: Glutathione-S-Transferase Protein. Lane 13: Maltose Binding Protein  Marker: Opal Pre-stained ladder Load: 10 µg of lysate or 50ng of purified protein per lane. Primary antibody: goat anti-GFP peroxidase conjugated antibody at 1:1,000 overnight at 4C. Secondary antibody: none

Polyclonal GFP Antibody (HRP, IHC, WB) LS‑C154191

Polyclonal GFP Antibody (HRP, IHC, WB) LS‑C154191

Note: This antibody replaces LS-C50846
Antibody:
Goat Polyclonal to Aequorea victoria GFP
Application:
IHC, WB, ELISA
Reactivity:
Aequorea victoria
Format:
Horseradish Peroxidase, Unmodified
Price
Catalog Number
$495 
LS-C154191-1
1 mg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Aequorea victoria GFP
Application:
IHC, WB, ELISA
Reactivity:
Aequorea victoria
Format:
Horseradish Peroxidase, Unmodified

Specifications

Description
GFP antibody LS-C154191 is an HRP-conjugated goat polyclonal antibody to aequorea victoria GFP. Validated for ELISA, IHC and WB.
Target
Aequorea victoria GFP
Synonyms
GFP
Host
Goat
Reactivity
Aequorea victoria (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Horseradish Peroxidase
Purification
Affinity chromatography
Modifications
Unmodified
Applications
  • IHC (1:500 - 1:2000)
  • Western blot (1:2000 - 1:5000)
  • ELISA (1:10000 - 1:50000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Peroxidase and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against Human, Mouse and Rat Serum Proteins.
Usage
Polyclonal anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with a monoclonal anti-GFP antibody using either form of the antibody as the capture or detection antibody. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E. coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Reconstitution
Reconstitute in 1 mL of deionized water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GFP
P42212 M62653 P42212

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Requested From: United States
Date Requested: 2/21/2020