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GFP Antibody (clone 9F9.F9, FITC) LS‑C154209

Catalog Number Size Price
LS-C154209-1 1 mg $710 
GFP Antibody Fluorescein Conjugated Western B, ot. Western Blot of GFP Antibody Fluorescein Conjugated Lane 1: GFP Load: 50 ng per lane Secondary antibody: Fluorescein Conjugated Mouse Anti-GFP at 1:1000 for 60 min at RT Block: 1% BSA-TTBS for 30 min at RT Predicted/Observed size: 28 kD, 28 kD. This image was taken for the unconjugated form of this product. Other forms have not been tested.

GFP Antibody (clone 9F9.F9, FITC) LS‑C154209

Antibody:
Mouse Monoclonal to Aequorea victoria GFP
Application:
IF, WB
Reactivity:
Aequorea victoria
Format:
FITC, Unmodified
Price
Catalog Number
$710 
LS-C154209-1
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Aequorea victoria GFP
Application:
IF, WB
Reactivity:
Aequorea victoria
Format:
FITC, Unmodified

Specifications

Description
GFP antibody LS-C154209 is an FITC-conjugated mouse monoclonal antibody to aequorea victoria GFP. Validated for IF and WB.
Target
Aequorea victoria GFP
Host
Mouse
Reactivity
Aequorea victoria (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal
Clone
9F9.F9
Conjugations
FITC
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:500 - 1:2500)
  • Western blot
Immunogen
The immunogen is a GST-Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Specificity
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum. Reactivity is observed against recombinant Green Fluorescent Protein (000-001-215, recombinant GFP from Aequorea victoria) by both Western blot and ELISA. No reaction is seen against RFP.
Usage
Monoclonal anti-GFP is designed to detect enhanced GFP and GFP containing recombinant proteins. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated monoclonal anti-GFP is well suited to titrate GFP in a sandwich ELISA in combination with a polyclonal anti-GFP antibody as the capture antibody. Only use the monoclonal form for the detection of enhanced or recombinant GFP. Polyclonal anti-GFP detects all variants of GFP tested to date. The biotin conjugated detection antibody is typically used with streptavidin conjugated HRP or other streptavidin conjugates. The use of polyclonal anti-GFP results in significant amplification of signal when fluorochrome conjugated polyclonal anti-GFP is used relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide, 1% BSA
Reconstitution
Reconstitute in deionized water
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GFP
P42212 M62653 P42212

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Anti-GFP Chemiluminescent Kit for Western blot. Known amounts of recombinant GFP protein ( were spiked into a HeLa cell-derived lysates (p/n W09-000-364) and separated by SDS-PAGE using a 4-20% gradient gel. Proteins were transferred onto a nitrocellulose membrane for 1 h at 100 mV. The membrane was blocked with TTBS (p/n MB-013) supplemented with 1% BSA (p/n BSA-50) for 1 h at 4? prior to probing the blot with the anti-GFP monoclonal antibody Anti-GFP (MOUSE) Monoclonal Antibody - 600-301-215 diluted 1:1000 for 40 min. Detection of the primary antibody by the HRP-conjugated anti-Mouse IgG (p/n LS-C60772) was performed at a dilution of 1:20000 for 1h at 4?. FemtoMax Super Sensitive Chemiluminescent Luminol Substrate was used for signal detection (see below).
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Requested From: United States
Date Requested: 8/22/2019
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