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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GFP Antibody (Biotin) LS‑C154288

Catalog Number Size Price
LS-C154288-100 100 µg (1 mg/ml) $425 
Antibody

GFP Antibody (Biotin) LS‑C154288

Antibody:
Rabbit Polyclonal to Aequorea victoria GFP
Application:
IHC, WB, ELISA
Reactivity:
Aequorea victoria
Format:
Biotin, Unmodified
Price
Catalog Number
$425 
LS-C154288-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Aequorea victoria GFP
Application:
IHC, WB, ELISA
Reactivity:
Aequorea victoria
Format:
Biotin, Unmodified

Specifications

Description
GFP antibody LS-C154288 is a biotin-conjugated rabbit polyclonal antibody to aequorea victoria GFP. Validated for ELISA, IHC and WB. Cited in 1 publication.
Target
Aequorea victoria GFP
Host
Rabbit
Reactivity
Aequorea victoria (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Biotin
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC (1:1000 - 1:5000)
  • Western blot (1:2000 - 1:10000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
The immunogen is a GST-Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Biotin and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse and Rat Serum Proteins.
Usage
Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide, 1% BSA
Reconstitution
Reconstitute in deionized water
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GFP
P42212 M62653 P42212

Publications (1)

ProMAT calibrator: A tool for reducing experimental bias in antibody microarrays. Zangar RC, Daly DS, White AM, Servoss SL, Tan RM, Collett JR. Journal of proteome research. 2009 8:3937-43.

Reviews (0)

Featured Products

Product
Species: Aequorea victoria
Applications: IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Product
Species: Aequorea victoria
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Product
Species: Aequorea victoria
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, ELISA
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Species: Aequorea victoria
Applications: IHC, Immunofluorescence, Western blot, ELISA
Anti-GFP Chemiluminescent Kit for Western blot. Known amounts of recombinant GFP protein ( were spiked into a HeLa cell-derived lysates (p/n W09-000-364) and separated by SDS-PAGE using a 4-20% gradient gel. Proteins were transferred onto a nitrocellulose membrane for 1 h at 100 mV. The membrane was blocked with TTBS (p/n MB-013) supplemented with 1% BSA (p/n BSA-50) for 1 h at 4? prior to probing the blot with the anti-GFP monoclonal antibody Anti-GFP (MOUSE) Monoclonal Antibody - 600-301-215 diluted 1:1000 for 40 min. Detection of the primary antibody by the HRP-conjugated anti-Mouse IgG (p/n LS-C60772) was performed at a dilution of 1:20000 for 1h at 4?. FemtoMax Super Sensitive Chemiluminescent Luminol Substrate was used for signal detection (see below).
Species: Aequorea victoria
Applications: IHC, IHC - Frozen, Immunofluorescence, Western blot, ELISA
Product
Species: Aequorea victoria
Applications: IHC, Western blot, ELISA

Requested From: United States
Date Requested: 8/22/2019
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