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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C154288-100 100 µg (1 mg/ml) $425 
GFP Antibody - Immuno-Fluorescence of Biotin Mouse anti-GFP antibody. Biotin mouse anti GFP used 1:5000 As referenced in:

Polyclonal GFP Antibody (Biotin, IHC, IF, WB) LS‑C154288

Polyclonal GFP Antibody (Biotin, IHC, IF, WB) LS‑C154288

Antibody:
Rabbit Polyclonal to Aequorea victoria GFP
Application:
IHC, IF, WB, ELISA
Reactivity:
Aequorea victoria
Format:
Biotin, Unmodified
Price
Catalog Number
$425 
LS-C154288-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Aequorea victoria GFP
Application:
IHC, IF, WB, ELISA
Reactivity:
Aequorea victoria
Format:
Biotin, Unmodified

Specifications

Description
GFP antibody LS-C154288 is a biotin-conjugated rabbit polyclonal antibody to aequorea victoria GFP. Validated for ELISA, IF, IHC and WB. Cited in 1 publication.
Target
Aequorea victoria GFP
Synonyms
GFP
Host
Rabbit
Reactivity
Aequorea victoria (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Biotin
Purification
Affinity chromatography
Modifications
Unmodified
Applications
  • IHC (1:1000 - 1:5000)
  • Immunofluorescence (1:5000)
  • Western blot (1:2000 - 1:10000)
  • ELISA (1:10000 - 1:50000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Anti-Green Fluorescent Protein (GFP) is produced by immunizing GFP containing fusion protein that corresponds to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Biotin and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse and Rat Serum Proteins.
Usage
Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GFP
P42212 M62653 P42212

LSBio Ratings

GFP Antibody (Biotin) for IHC, IF/Immunofluorescence, WB/Western, ELISA LS-C154288 has an LSBio Rating of
Publications (4)

Publications (1)

ProMAT calibrator: A tool for reducing experimental bias in antibody microarrays. Zangar RC, Daly DS, White AM, Servoss SL, Tan RM, Collett JR. Journal of proteome research. 2009 8:3937-43.

Customer Reviews (0)


Requested From: United States
Date Requested: 3/29/2020