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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TP53 / p53 Antibody (phospho‑Ser15) LS‑C117442
P53 antibody LS-C117442 is an unconjugated rabbit polyclonal antibody to p53 (TP53) from human and rat. Validated for IHC, IP, Peptide-ELISA and WB.
Catalog
Size
Price
LS-C117442-50
50 µl
Unavailable
LS-C117442-100
100 µl
Unavailable

Popular TP53 / p53 Products

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Anti-TP53 / p53 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4557 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Applications: Western blot, Flow Cytometry, ELISA

Product Description

P53 antibody LS-C117442 is an unconjugated rabbit polyclonal antibody to p53 (TP53) from human and rat. Validated for IHC, IP, Peptide-ELISA and WB.
About TP53 / p53
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. P04637 NM_000546 NP_000537.3

TP53 Antibody, BCC7 Antibody, Cellular tumor antigen p53 Antibody, p53 Antibody, TRP53 Antibody, p53 tumor suppressor Antibody, Phosphoprotein p53 Antibody, Antigen NY-CO-13 Antibody, Tumor protein p53 Antibody, Tumor suppressor p53 Antibody, LFS1 Antibody


Specifications

Target
Human TP53 / p53
Host
Rabbit
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation
  • Peptide Enzyme-Linked Immunosorbent Assay (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
TP53 / p53 antibody was raised against synthetic peptide from human p53 around the phosphorylation site of Ser15.
Epitope
pSer15
Specificity
p53 (Phospho-Ser15) Antibody detects endogenous levels of p53 only when phosphorylated at serine15.
Presentation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Storage
Store at -20°C for up to 1 year.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded human breast carcinoma, using p53 (Phospho-Ser15) Antibody. The picture on the right is treated with the synthesized peptide.
IHC of paraffin-embedded human breast carcinoma, using p53 (Phospho-Ser15) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from HeLa cells treated with HU, using p53 (Phospho-Ser15) Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from HeLa cells treated with HU, using p53 (Phospho-Ser15) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry

IHC of paraffin-embedded human breast carcinoma, using p53 (Phospho-Ser15) Antibody. The picture on the right is treated with the synthesized peptide.
IHC of paraffin-embedded human breast carcinoma, using p53 (Phospho-Ser15) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from HeLa cells treated with HU, using p53 (Phospho-Ser15) Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from HeLa cells treated with HU, using p53 (Phospho-Ser15) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry

IHC of paraffin-embedded human breast carcinoma, using p53 (Phospho-Ser15) Antibody. The picture on the right is treated with the synthesized peptide.
IHC of paraffin-embedded human breast carcinoma, using p53 (Phospho-Ser15) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from HeLa cells treated with HU, using p53 (Phospho-Ser15) Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from HeLa cells treated with HU, using p53 (Phospho-Ser15) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry

IHC of paraffin-embedded human breast carcinoma, using p53 (Phospho-Ser15) Antibody. The picture on the right is treated with the synthesized peptide.
IHC of paraffin-embedded human breast carcinoma, using p53 (Phospho-Ser15) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from HeLa cells treated with HU, using p53 (Phospho-Ser15) Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from HeLa cells treated with HU, using p53 (Phospho-Ser15) Antibody. The lane on the right is treated with the synthesized peptide.


Requested From: 
Date Requested: 6/21/2018

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