Monoclonal Mouse anti‑Human TP53 / p53 Antibody (clone SPM589, Azide‑free,BSA‑free, IHC, IF, WB) LS‑C392510
Monoclonal Mouse anti‑Human TP53 / p53 Antibody (clone SPM589, Azide‑free,BSA‑free, IHC, IF, WB) LS‑C392510
Antibody:
TP53 / p53 Mouse anti-Human Monoclonal (Azide-free, BSA-free) (SPM589) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Format:
Unconjugated, Azide-free, BSA-free
Overview
Antibody:
TP53 / p53 Mouse anti-Human Monoclonal (Azide-free, BSA-free) (SPM589) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Format:
Unconjugated, Azide-free, BSA-free
Specifications
Description
P53 antibody LS-C392510 is an unconjugated mouse monoclonal antibody to human p53 (TP53). Validated for Flow, IF, IHC and WB.
Target
Human TP53 / p53
Synonyms
TP53 | BCC7 | Cellular tumor antigen p53 | p53 | TRP53 | p53 tumor suppressor | Phosphoprotein p53 | Antigen NY-CO-13 | Tumor protein p53 | Tumor suppressor p53 | LFS1
Reactivity
Human
(tested or 100% immunogen sequence identity)
Non-Reactivity
Mouse, Rat
Clonality
IgG2a
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Azide-free, BSA-free.
Also available
Unmodified.
Immunogen
Recombinant human wild type p53 protein.
Specificity
Human TP53 / p53
Applications
- IHC
- IHC - Paraffin (0.5 - 1 µg/ml)
- Immunofluorescence (1 - 2 µg/ml)
- Western blot (0.5 - 1 µg/ml)
- Flow Cytometry (0.5 - 1 µg/10E6 cells)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. The optimal dilution of the anti-p53 antibody for each application should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Storage
Store at 2°C to 8°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA, Chromatin Immunoprecipitation
Species:
Human
Applications:
IHC
Species:
Mouse, Human, Rat, Bovine, Hamster, Chicken
Applications:
IHC, Western blot, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, ELISA
Species:
Human
Applications:
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/26/2024