LSBio
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA and Assay Kits
  • All Kits
  • Assay Kits
  • Traditional ELISA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Phospho-Specific ELISA Kits
  • ELISA Development Kits
  • Chemiluminescent CLIA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Biochemicals
  • All Biochemicals

  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Protocols
  • Secure Logins
  • Login to Our Secure ShareFile Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login ▾
Wish List
View Cart

Anti-TP53 / p53 Antibody (clone 5D9) LS-C172980

Catalog Size Price
LS-C172980-100 100 µl (0.6 mg/ml) Unavailable

Related Products

699 TP53 / p53 Antibodies

Most Popular TP53 / p53 Antibodies

Anti-TP53 / p53 Antibody (clone BP53-12) IHC-plus™ LS-B431
Mouse Monoclonal [clone BP53-12] (IgG2a,k) to Human TP53 / p53
Human
IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-TP53 / p53 Antibody LS-C50534
Rabbit Polyclonal to Human TP53 / p53
Human
IHC
Unconjugated
Immunohistochemistry Image
Anti-TP53 / p53 Antibody (clone PAb240, Azide-free) LS-C87442
Mouse Monoclonal [clone PAb240] (IgG1) to Mouse TP53 / p53
Mouse, Human
IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated, Azide-free
Immunohistochemistry Image
Anti-TP53 / p53 Antibody (clone PAb240, Biotin) LS-C134560
Mouse Monoclonal [clone PAb240] (IgG1,k) to Mouse TP53 / p53
Mouse, Human, Rat, Bovine, Hamster, Chicken
IHC, Western blot, Flow Cytometry
Biotin Conjugated
Flow Cytometry Image
Anti-TP53 / p53 Antibody (clone 6F2D3) LS-C203312
Mouse Monoclonal [clone 6F2D3] (IgG1) to Human TP53 / p53
Human
Western blot, Flow Cytometry, ELISA
Unconjugated

100% Guaranteed
Mouse Monoclonal [clone 5D9] (IgG1) to Human TP53 / p53
Human, Monkey
Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human TP53 / p53
Mouse
Human, Monkey (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [5D9]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • Immunofluorescence (1:100)
  • Western blot (1:500 - 1:2000)
  • Flow Cytometry (1:100)

Specificity and Use

TP53 / p53 antibody was raised against full length human recombinant protein of human TP53 (NP_000537) produced in HEK293T cell.
Human p53

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About TP53 / p53

P04637 NM_000546 NP_000537.3

TP53 Antibody, BCC7 Antibody, Cellular tumor antigen p53 Antibody, p53 Antibody, TRP53 Antibody, p53 tumor suppressor Antibody, Phosphoprotein p53 Antibody, Antigen NY-CO-13 Antibody, Tumor protein p53 Antibody, Tumor suppressor p53 Antibody, LFS1 Antibody

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases.

Immunofluorescence

Immunofluorescence
Anti-TP53 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TP53.

Western blot

Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TP53 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TP53.

Western blot

Western blot
Western blot of extracts (35 ug) from 9 different cell lines by using anti-TP53 monoclonal antibody.

Flow Cytometry

Flow Cytometry
Flow cytometry of Jurkat cells, using anti-TP53 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow Cytometry
Flow cytometry of HeLa cells, using anti-TP53 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TP53 antibody, and then analyzed by flow cytometry.

Requested From: 
Date Requested: 12/16/2017

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2017 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number