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Anti-p66 / SHC Antibody (aa371-420) LS-C81553

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl - $365
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular p66 / SHC Antibodies

Anti-p66 / SHC Antibody (phospho-Ser36, clone 6E10) LS-C10125
Mouse Monoclonal [clone 6E10] (IgG1) to Human p66 / SHC
Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Pig
Western blot, ELISA
Unconjugated
Anti-p66 / SHC Antibody (phospho-Tyr427) LS-C34237
Rabbit Polyclonal (IgG) to Human p66 / SHC
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image
Anti-p66 / SHC Antibody (clone 3F4) IHC-plus™ LS-B4416
Mouse Monoclonal [clone 3F4] (IgG,k) to Human p66 / SHC
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA, Proximity Ligation Assay
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human p66 / SHC
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Xenopus
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human p66 / SHC
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Xenopus (tested or 100% immunogen sequence identity)
Chicken, Zebrafish (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

p66 / SHC antibody was raised against synthetic peptide located between aa371-420 of human SHC1 (P29353, NP_003020). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Zebra finch, Xenopus (100%); Galago, Turkey, Chicken, Stickleback, Zebrafish (92%); Panda (85%).
aa371-420
Human SHC1
ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About p66 / SHC

P29353 NM_003029 NP_003020.2

SHC1 Antibody, p46 shc Antibody, p52 shc Antibody, SH2 domain protein C1 Antibody, p66 Antibody, SHCA Antibody, SHC Antibody, SHC-transforming protein 1 Antibody, SHC-transforming protein 3 Antibody, SHC-transforming protein A Antibody

Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals.

Western blot

Western blot
Fetal lung cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/3/2016

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