SHC antibody LS-C10125 is an unconjugated mouse monoclonal antibody to SHC (p66) from human, mouse, rat and other species. Validated for ELISA and WB.
Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Pig
(tested or 100% immunogen sequence identity)
- Western blot (1 µg/ml)
p66 / SHC antibody was raised against synthetic peptide corresponding to aa 33-39 (ELPpSPSA) of shc phosphorylated at Ser36. Molecular Weight: 66kD.
Recognizes human shc phosphorylated at Ser36. Species cross-reactivity: mouse and canine.
Suitable for use in ELISA and Western Blot. Western Blot: 1 ug/ml for HRPO/ECL detection. Use Casein/Tween-20 based blocking and Blot incubation buffers. Positive control: S1010-92G1: Shc, phosphorylated (Ser36) HepG2 Cell Lysate, EGF Treated. Add 62 ul SDS-PAGE sample buffer and incubate at 90?C for 5 minutes. Aliquot and store at -20?C.
PBS, pH 7.2, 0.1% sodium azide, PEG, sucrose
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.