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p66 / SHC Antibody (phospho-Ser36, clone 6E10) LS-C10125
SHC antibody LS-C10125 is an unconjugated mouse monoclonal antibody to SHC (p66) from human, mouse, rat and other species. Validated for ELISA and WB.
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SHC antibody LS-C10125 is an unconjugated mouse monoclonal antibody to SHC (p66) from human, mouse, rat and other species. Validated for ELISA and WB.
Target
Human p66 / SHC
Host
Mouse
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Pig
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
6E10
Conjugations
Unconjugated
Purification
Gel filtration
Modifications
Unmodified
Applications
- Western blot (1 µg/ml)
- ELISA
Immunogen
p66 / SHC antibody was raised against synthetic peptide corresponding to aa 33-39 (ELPpSPSA) of shc phosphorylated at Ser36. Molecular Weight: 66kD.
Epitope
pSer36
Specificity
Recognizes human shc phosphorylated at Ser36. Species cross-reactivity: mouse and canine.
Usage
Suitable for use in ELISA and Western Blot. Western Blot: 1 ug/ml for HRPO/ECL detection. Use Casein/Tween-20 based blocking and Blot incubation buffers. Positive control: S1010-92G1: Shc, phosphorylated (Ser36) HepG2 Cell Lysate, EGF Treated. Add 62 ul SDS-PAGE sample buffer and incubate at 90?C for 5 minutes. Aliquot and store at -20?C.
Presentation
PBS, pH 7.2, 0.1% sodium azide, PEG, sucrose
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About p66 / SHC
Popular p66 / SHC Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA, Proximity Ligation Assay
Species:
Human, Mouse, Rat
Applications:
Western blot, Immunoprecipitation, ELISA
Species:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Xenopus
Applications:
Western blot
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Requested From: United States
Date Requested: 4/21/2019
Date Requested: 4/21/2019
