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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C10125-50 50 µg $545 
LS-C10125-100 100 µg $890 
Antibody

Monoclonal p66 / SHC Antibody (clone 6E10, phospho‑Ser36, WB) LS‑C10125

Monoclonal p66 / SHC Antibody (clone 6E10, phospho‑Ser36, WB) LS‑C10125

Antibody:
Mouse Monoclonal to Human p66 / SHC
Application:
WB, ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$545 
LS-C10125-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Mouse Monoclonal to Human p66 / SHC
Application:
WB, ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Pig
Format:
Unconjugated, Unmodified

Specifications

Description
SHC antibody LS-C10125 is an unconjugated mouse monoclonal antibody to SHC (p66) from human, mouse, rat and other species. Validated for ELISA and WB.
Target
Human p66 / SHC
Synonyms
SHC1 | p46 shc | p52 shc | SH2 domain protein C1 | p66 | SHCA | SHC | SHC-transforming protein 1 | SHC-transforming protein 3 | SHC-transforming protein A
Host
Mouse
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Pig (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
6E10
Conjugations
Unconjugated
Purification
Gel filtration
Modifications
Unmodified
Applications
  • Western blot (1 µg/ml)
  • ELISA
Immunogen
Synthetic peptide corresponding to aa 33-39 (ELPpSPSA) of shc phosphorylated at Ser36. Molecular Weight: 66kD.
Epitope
pSer36
Specificity
Recognizes human shc phosphorylated at Ser36. Species cross-reactivity: mouse and canine.
Usage
Suitable for use in ELISA and Western Blot. Western Blot: 1 ug/ml for HRPO/ECL detection. Use Casein/Tween-20 based blocking and Blot incubation buffers. Positive control: S1010-92G1: Shc, phosphorylated (Ser36) HepG2 Cell Lysate, EGF Treated. Add 62 ul SDS-PAGE sample buffer and incubate at 90?C for 5 minutes. Aliquot and store at -20?C.
Presentation
PBS, pH 7.2, 0.1% sodium azide, PEG, sucrose
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About p66 / SHC
P29353 NM_003029 NP_003020.2

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Requested From: United States
Date Requested: 2/28/2020