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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B4416-50 50 µg (0.42 mg/ml) $495 
p66 / SHC Antibody - Anti-SHC1 antibody IHC of human colon, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 1 ug/ml.
p66 / SHC Antibody - Anti-SHC1 antibody IHC of human adipocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 1 ug/ml.
p66 / SHC Antibody - Anti-SHC1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 1 ug/ml.
p66 / SHC Antibody - Immunofluorescence of monoclonal antibody to SHC1 on A-431 cells (antibody concentration 10 ug/ml).
p66 / SHC Antibody - SHC1 monoclonal antibody clone 3F4 Western blot of SHC1 expression in A-431.
p66 / SHC Antibody - Detection limit for recombinant GST tagged SHC1 is approximately 0.03 ng/ml as a capture antibody.
p66 / SHC Antibody - Proximity Ligation Analysis (PLA) of protein-protein interactions between PIK3R1 and SHC1 HeLa cells were stained with anti-PIK3R1 rabbit purified polyclonal 1:1200 and anti-SHC1 mouse monoclonal antibody 1:50. Signals were detected by Duolink 30 Detection.
p66 / SHC Antibody - Anti-SHC1 antibody IHC of human colon, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 1 ug/ml.
p66 / SHC Antibody - Anti-SHC1 antibody IHC of human adipocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 1 ug/ml.
p66 / SHC Antibody - Anti-SHC1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 1 ug/ml.
p66 / SHC Antibody - Immunofluorescence of monoclonal antibody to SHC1 on A-431 cells (antibody concentration 10 ug/ml).
p66 / SHC Antibody - SHC1 monoclonal antibody clone 3F4 Western blot of SHC1 expression in A-431.
p66 / SHC Antibody - Detection limit for recombinant GST tagged SHC1 is approximately 0.03 ng/ml as a capture antibody.
p66 / SHC Antibody - Proximity Ligation Analysis (PLA) of protein-protein interactions between PIK3R1 and SHC1 HeLa cells were stained with anti-PIK3R1 rabbit purified polyclonal 1:1200 and anti-SHC1 mouse monoclonal antibody 1:50. Signals were detected by Duolink 30 Detection.
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IHCPlus™ Monoclonal p66 / SHC Antibody (clone 3F4, IHC, IF, WB) LS‑B4416

IHCPlus™ Monoclonal p66 / SHC Antibody (clone 3F4, IHC, IF, WB) LS‑B4416

Note: This antibody replaces LS-C105026
Antibody:
Mouse Monoclonal to Human p66 / SHC
Application:
IHC, IHC-P, IF, WB, ELISA, PLA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$495 
LS-B4416-50
50 µg
0.42 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Mouse Monoclonal to Human p66 / SHC
Application:
IHC, IHC-P, IF, WB, ELISA, PLA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
SHC antibody LS-B4416 is an unconjugated mouse monoclonal antibody to human SHC (p66). Validated for ELISA, IF, IHC, PLA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human p66 / SHC
Synonyms
SHC1 | p46 shc | p52 shc | SH2 domain protein C1 | p66 | SHCA | SHC | SHC-transforming protein 1 | SHC-transforming protein 3 | SHC-transforming protein A
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG,k Monoclonal
Clone
3F4
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA
  • Proximity Ligation Assay
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
SHC1 (AAH33925, 171 aa ~ 280 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Usage
Western Blot (Cell lysate) - positive control A-431. Immunofluorescence (10 ug/ml) - positive control A-431 cells. Sandwich ELISA (Recombinant protein). In situ Proximity Ligation Assay (Cell).
Presentation
PBS, pH 7.4
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About p66 / SHC
P29353 NM_003029 NP_003020.2

Validation

p66 / SHC Antibody - Anti-SHC1 antibody IHC of human colon, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 1 ug/ml.
Anti-SHC1 antibody IHC of human colon, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 1 ug/ml.
Anti-SHC1 antibody IHC of human colon, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 1 ug/ml.

p66 / SHC Antibody - Anti-SHC1 antibody IHC of human adipocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 1 ug/ml.
Anti-SHC1 antibody IHC of human adipocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 1 ug/ml.
Anti-SHC1 antibody IHC of human adipocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 1 ug/ml.

p66 / SHC Antibody - Anti-SHC1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 1 ug/ml.
Anti-SHC1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 1 ug/ml.
Anti-SHC1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 1 ug/ml.

LSBio Ratings

IHC-plus™ p66 / SHC Antibody (clone 3F4) for IHC, IF/Immunofluorescence, WB/Western, ELISA LS-B4416 has an LSBio Rating of
Laboratory Validation Score (4)

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Requested From: United States
Date Requested: 4/3/2020