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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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p66 / SHC

SHC (Src homology 2 domain containing) transforming protein 1

Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span (By similarity). Participates in signaling downstream of the angiopoietin receptor TEK/TIE2, and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis.

Gene Name: SHC (Src homology 2 domain containing) transforming protein 1
Synonyms: SHC1, p46 shc, p52 shc, SH2 domain protein C1, p66, SHCA, SHC, SHC-transforming protein 1, SHC-transforming protein 3, SHC-transforming protein A
Target Sequences: NM_003029 NP_003020.2 P29353

Publications (1)

1
p66Shc: A novel biomarker of tubular oxidative injury in patients with diabetic nephropathy. Xu X, Zhu X, Ma M, Han Y, Hu C, Yuan S, Yang Y, Xiao L, Liu F, Kanwar YS, Sun L. Scientific reports. 2016 6:29302. (WB; Human) [Full Text Article] [PubMed:27377870] [PMC:PMC4932503] Related Antibodies: LS-C10119.

Filtered by: None
Target
p66 / SHC (80)
Reactivity
Human (69)
Mouse (52)
Rat (41)
Bat (5)
Bovine (11)
Chimpanzee (4)
Dog (9)
Gibbon (3)
Guinea pig (4)
Hamster (4)
Horse (10)
Monkey (10)
Orangutan (3)
Pig (11)
Rabbit (4)
Sheep (1)
Xenopus (6)
Zebrafish (2)
Application
IHC (40)
IHC-Fr (2)
IHC-P (21)
WB (73)
Flo (4)
ELISA (27)
ICC (1)
IF (11)
IP (6)
Peptide-ELISA (4)
PLA (1)
Host
rabbit (68)
mouse (12)
Isotype
IgG (42)
IgG,k (1)
IgG1 (9)
IgG1,k (2)
Clonality
monoclonal mc (13)
polyclonal pc (67)
Clone
1D3 (1)
1H8 (1)
2F7C7 (1)
3A1 (1)
3F4 (1)
3H1 (1)
6E10 (1)
EP332Y (1)
Format
AP Conjugated (1)
APC (1)
APC Conjugated (2)
Azide-free (1)
Biotin Conjugated (4)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (4)
HRP Conjugated (4)
PE Conjugated (2)
Unconjugated (61)
Epitope
pTyr427 (9)
aa272-559 (8)
aa378-405 (8)
pTyr349 (7)
aa371-420 (4)
290-370 aa (2)
370-450 aa (2)
pTyr239/Tyr240 (2)
pTyr317 (2)
10-90 aa (1)
Glu343 (1)
Leu421 (1)
N-Terminus (1)
SH2 Domain (1)
aa300-350 (1)
aa314-320 (1)
aa315-364 (1)
aa366-473 (1)
aa393-442 (1)
aa424-440 (1)
aa536-564 (1)
pSer36 (1)
Publications
No (79)
Yes (1)
Showing 1-25 of 80 results

IHC-plus™ p66 / SHC Antibody (clone 3F4) LS-B4416
Mouse Monoclonal [clone 3F4] (IgG,k) to Human p66 / SHC
Human
ELISA, IF, IHC, IHC-P, PLA, WB
Unconjugated
50µg  $495.00
Anti-SHC1 antibody IHC of human adipocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 1 ug/ml.
Anti-SHC1 antibody IHC of human colon, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 1 ug/ml.

p66 / SHC Antibody (N-Terminus) LS-C10119
Publications (1)
Publications (1)
Mouse Monoclonal (IgG1) to Human p66 / SHC
Human
ELISA, WB
Unconjugated
50µg  $545.00; 100µg  $890.00

p66 / SHC Antibody LS-C62055
Mouse Monoclonal (IgG1,k) to Human p66 / SHC
Mouse, Rat, Human
ELISA, IP, WB
Unconjugated
100µl 1mg/ml  $305.00
Western blot Image

IHC-plus™ p66 / SHC Antibody (SH2 Domain, clone EP332Y) LS-B7922
Rabbit Monoclonal [clone EP332Y] (IgG) to Human p66 / SHC
Mouse, Rat, Human
Flo, IHC, IHC-P, WB
Unconjugated
50µl  $425.00
Anti-p66 / SHC antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Western blot analysis on MCF-7 cell lysate using anti-SHC1 antibody, dilution 1:2000.

p66 / SHC Antibody (phospho-Ser36, clone 6E10) LS-C10125
Mouse Monoclonal [clone 6E10] (IgG1) to Human p66 / SHC
Mouse, Dog, Bovine, Rat, Pig, Horse, Human, Monkey
ELISA, WB
Unconjugated
50µg  $545.00; 100µg  $890.00

p66 / SHC Antibody (clone 3A1) LS-C115098
Mouse Monoclonal [clone 3A1] (IgG1) to Human p66 / SHC
Human
IF, IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $345.00
IHC of paraffin-embedded breast tissue using anti-SHC1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded colon tissue using anti-SHC1 mouse monoclonal antibody. (Dilution 1:50).

p66 / SHC Antibody (aa371-420) LS-C81553
Rabbit Polyclonal (IgG) to Human p66 / SHC
Xenopus, Bat, Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Hamster, Pig, Horse, Human, Monkey
WB
Unconjugated
100µl 0.5mg/ml  $365.00
Fetal lung cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

p66 / SHC Antibody (aa424-440) LS-C313035
Rabbit Polyclonal to Human p66 / SHC
Mouse, Rat, Human
ICC, IHC, IHC-Fr, IHC-P, WB
Unconjugated
100µg 200µl 0.5mg/ml  $305.00
p66 / SHC antibody. IHC(P): Rat Brain Tissue.
p66 / SHC antibody. IHC(P): Human Lung Cancer Tissue.

p66 / SHC Antibody (aa366-473) LS-C152718
Rabbit Polyclonal (IgG) to Human p66 / SHC
Mouse, Rat, Pig, Human
IHC, IP, WB
Unconjugated
250µg 1mg/ml  $475.00
WB: no primary antibody (lane 1); and A431 cell lysate probed with anti-SHC (lane 2)

p66 / SHC Antibody (phospho-Tyr427) LS-C91357
Rabbit Polyclonal (IgG) to Human p66 / SHC
Human
IHC, WB
Unconjugated
100µg  $495.00

p66 / SHC Antibody (clone 1H8) LS-C115116
Mouse Monoclonal [clone 1H8] (IgG1) to Human p66 / SHC
Human
IF, IHC, IHC-P, WB
Unconjugated
100µl 0.92mg/ml  $345.00
IHC of paraffin-embedded colon tissue using anti-SHC1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-SHC1 mouse monoclonal antibody. (Dilution 1:50).

p66 / SHC Antibody LS-C752848
Rabbit Polyclonal (IgG) to Human p66 / SHC
Human
ELISA, IF, IHC, WB
Unconjugated
100µg  $355.00

p66 / SHC Antibody LS-C752850
Rabbit Polyclonal (IgG) to Human p66 / SHC
Human
ELISA, IF, IHC, WB
Unconjugated
100µg  $355.00

p66 / SHC Antibody LS-C490069
Rabbit Polyclonal (IgG) to Human p66 / SHC
Mouse, Rat, Human
IHC, IHC-Fr, IHC-P, WB
Unconjugated
100µg  $435.00
Immunohistochemistry - Paraffin Image
Immunohistochemistry - Paraffin Image

p66 / SHC Antibody (clone 1D3) LS-C115115
Mouse Monoclonal [clone 1D3] (IgG1) to Human p66 / SHC
Flo, IF, WB
Unconjugated
100µl 0.93mg/ml  $345.00
Anti-SHC1 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SHC1.
Immunofluorescent staining of HT29 cells using anti-SHC1 mouse monoclonal antibody.

p66 / SHC Antibody (phospho-Tyr427) LS-C203000
Rabbit Polyclonal to Human p66 / SHC
Mouse, Rat, Human
IF, IHC, IHC-P, WB
Unconjugated
100µg 1mg/ml  $315.00
Immunohistochemistry of paraffin-embedded human breast carcinoma tissue, using Rabbit Anti-Shc1 (Phospho-Tyr427) Polyclonal Antibody.
Western blot of extract from 293 cells, using Rabbit Anti-Shc1 (Ab-427) Polyclonal Antibody (lane 1 and 2) and Rabbit Anti-Shc1 (Phospho-Tyr427) Polyclonal Antibody (lane 3 and 4).

p66 / SHC Antibody (clone 3H1) LS-C115143
Mouse Monoclonal [clone 3H1] (IgG1) to Human p66 / SHC
Human
Flo, IF, WB
Unconjugated
100µl 0.86mg/ml  $345.00
Anti-SHC1 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SHC1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHC1.

p66 / SHC Antibody LS-C499763
Rabbit Polyclonal (IgG) to Human p66 / SHC
Human
ELISA, IF, IHC
Unconjugated
50µg  $285.00; 100µg  $385.00
Immunohistochemistry of paraffin-embedded human prostate cancer using SHC1 Antibody at dilution of 1:100
Immunofluorescent analysis of Hela cells using SHC1 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

p66 / SHC Antibody (290-370 aa) LS-C386066
Rabbit Polyclonal (IgG) to Human p66 / SHC
Mouse, Rat, Human
ELISA, IHC, IP, WB
Unconjugated
100µl 1mg/ml  $265.00

p66 / SHC Antibody (phospho-Tyr427) LS-C335920
Rabbit Polyclonal (IgG) to Human p66 / SHC
IF, IHC, WB
Unconjugated
50µl  $265.00; 100µl  $375.00; 200µl  $495.00
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue.
Immunofluorescence staining of methanol-fixed Hela cells.

p66 / SHC Antibody (aa536-564) LS-C357582
Rabbit Polyclonal to Human p66 / SHC
Mouse, Sheep, Rat, Pig, Horse, Bat, Chimpanzee, Human, Monkey
IHC, IHC-P, WB
Unconjugated
100µg 200µl 0.5mg/ml  $325.00
SHC1 antibody IHC-paraffin: Mouse Brain Tissue.
SHC1 antibody IHC-paraffin: Rat Brain Tissue.

p66 / SHC Antibody (phospho-Tyr317) LS-C10121
Mouse Monoclonal (IgG1) to Human p66 / SHC
Xenopus, Zebrafish, Pig, Horse, Dog, Bovine, Human, Monkey
ELISA, IHC, WB
Unconjugated
100µg  $815.00

p66 / SHC Antibody (Glu343) LS-C176567
Rabbit Polyclonal to Human p66 / SHC
Mouse, Rat, Human
IHC, IHC-P, IP, WB
Unconjugated
100µl  $345.00
IHC of Shc (E343) pAb in paraffin-embedded human breast carcinoma tissue.
Western blot of Shc (E343) pAb in extracts from HeLa cells.

p66 / SHC Antibody (aa393-442) LS-C117535
Rabbit Polyclonal (IgG) to Human p66 / SHC
Mouse, Rat, Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Shc Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HeLa cells, treated with Calyculin A 50nM 15', using Shc Antibody. The lane on the right is blocked with the synthesized peptide.

p66 / SHC Antibody (aa315-364) LS-C117534
Rabbit Polyclonal (IgG) to Human p66 / SHC
Mouse, Rat, Human
IHC, IP, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Shc Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HeLa cells, treated with Calyculin A 50nM 15', using Shc Antibody. The lane on the right is blocked with the synthesized peptide.
Showing 1-25 of 80 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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