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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MCL1 / MCL 1 Antibody IHC‑plus™ LS‑B3686
Note: This antibody replaces LS-C63135
MCL 1 antibody LS-B3686 is an unconjugated rabbit polyclonal antibody to MCL 1 (MCL1) from human, dog, guinea pig and other species. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B3686-50
50 µg (1 mg/ml)
Unavailable

Popular MCL1 / MCL 1 Products

Anti-MCL1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B586 concentration 2 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Western blot
IHC of paraffin-embedded human lymph node showing cytoplasmic localization using MCL-1 antibody with DAB staining.
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Anti-MCL1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5215 dilution 1:25.
Species: Human
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Antibody
Species: Human, Mouse
Applications: IHC, Western blot, Flow Cytometry, ELISA
Anti-MCL1 / MCL 1 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10210 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry

Product Description

MCL 1 antibody LS-B3686 is an unconjugated rabbit polyclonal antibody to MCL 1 (MCL1) from human, dog, guinea pig and other species. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About MCL1 / MCL 1
Myeloid cell leukimia-1 (Mcl-1) is a member of the Bcl-2 family of proteins that can act to promote cell survival. While the mechanism by which Mcl-1 inhibits apoptosis is not known, it is thought that it may heterodimerize and neutralize pro-apoptotic members of the Bcl-2 family such as Bim or Bak. Mcl-1 was originally identified in differentiating myeloid cells, but has since been shown to be expressed in multiple cell types. Q07820 NM_021960 NP_068779.1

MCL1 Antibody, BCL2L3 Antibody, Bcl-2-like protein 3 Antibody, Bcl-2-related protein EAT/mcl1 Antibody, Bcl2-L-3 Antibody, MCL1S Antibody, MCL1L Antibody, Myeloid cell leukemia ES Antibody, MCL 1 Antibody, MCL1-ES Antibody, TM Antibody, EAT Antibody, Mcl-1 Antibody


Specifications

Target
Human MCL1 / MCL 1
Host
Rabbit
Reactivity
Human, Monkey, Dog, Guinea pig, Pig, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
MCL1 / MCL 1 antibody was raised against synthetic peptide derived from the sequence of human Mcl-1, conjugated to KLH.
Usage
Immunohistochemistry: LS-B3686 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3686 was determined to be 10 ug/ml.
Presentation
PBS, 0.09% sodium azide
Storage
Store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-MCL1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3686 concentration 10 ug/ml.
Anti-MCL1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3686 concentration 10 ug/ml.

Immunohistochemistry

Anti-MCL1 antibody IHC of human small intestine, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3686 concentration 10 ug/ml.
Anti-MCL1 antibody IHC of human small intestine, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3686 concentration 10 ug/ml.

Immunofluorescence

Confocal immunofluorescent staining of HeLa cells using Mcl-1 polyclonal antibody (green); nuclei are stained in blue pseudocolor using DRAQ5.
Confocal immunofluorescent staining of HeLa cells using Mcl-1 polyclonal antibody (green); nuclei are stained in blue pseudocolor using DRAQ5.

Western blot

Western blot of human epidermoid carcinoma A431 cell lysate, probed with Mcl-1 polyclonal antibody.
Western blot of human epidermoid carcinoma A431 cell lysate, probed with Mcl-1 polyclonal antibody.

Immunohistochemistry

Anti-MCL1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3686 concentration 10 ug/ml.
Anti-MCL1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3686 concentration 10 ug/ml.

Immunohistochemistry

Anti-MCL1 antibody IHC of human small intestine, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3686 concentration 10 ug/ml.
Anti-MCL1 antibody IHC of human small intestine, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3686 concentration 10 ug/ml.

Immunofluorescence

Confocal immunofluorescent staining of HeLa cells using Mcl-1 polyclonal antibody (green); nuclei are stained in blue pseudocolor using DRAQ5.
Confocal immunofluorescent staining of HeLa cells using Mcl-1 polyclonal antibody (green); nuclei are stained in blue pseudocolor using DRAQ5.

Western blot

Western blot of human epidermoid carcinoma A431 cell lysate, probed with Mcl-1 polyclonal antibody.
Western blot of human epidermoid carcinoma A431 cell lysate, probed with Mcl-1 polyclonal antibody.

Immunohistochemistry

Anti-MCL1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3686 concentration 10 ug/ml.
Anti-MCL1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3686 concentration 10 ug/ml.

Immunohistochemistry

Anti-MCL1 antibody IHC of human small intestine, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3686 concentration 10 ug/ml.
Anti-MCL1 antibody IHC of human small intestine, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3686 concentration 10 ug/ml.

Immunofluorescence

Confocal immunofluorescent staining of HeLa cells using Mcl-1 polyclonal antibody (green); nuclei are stained in blue pseudocolor using DRAQ5.
Confocal immunofluorescent staining of HeLa cells using Mcl-1 polyclonal antibody (green); nuclei are stained in blue pseudocolor using DRAQ5.

Western blot

Western blot of human epidermoid carcinoma A431 cell lysate, probed with Mcl-1 polyclonal antibody.
Western blot of human epidermoid carcinoma A431 cell lysate, probed with Mcl-1 polyclonal antibody.

Immunohistochemistry

Anti-MCL1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3686 concentration 10 ug/ml.
Anti-MCL1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3686 concentration 10 ug/ml.

Immunohistochemistry

Anti-MCL1 antibody IHC of human small intestine, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3686 concentration 10 ug/ml.
Anti-MCL1 antibody IHC of human small intestine, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3686 concentration 10 ug/ml.

Immunofluorescence

Confocal immunofluorescent staining of HeLa cells using Mcl-1 polyclonal antibody (green); nuclei are stained in blue pseudocolor using DRAQ5.
Confocal immunofluorescent staining of HeLa cells using Mcl-1 polyclonal antibody (green); nuclei are stained in blue pseudocolor using DRAQ5.

Western blot

Western blot of human epidermoid carcinoma A431 cell lysate, probed with Mcl-1 polyclonal antibody.
Western blot of human epidermoid carcinoma A431 cell lysate, probed with Mcl-1 polyclonal antibody.


Requested From: 
Date Requested: 6/17/2018

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