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IHCPlus™ MCL1 / MCL 1 Antibody Monoclonal Flo,IF,IHC,WB LS‑B10210

Catalog Number Size Price
LS-B10210-50 50 µl (0.9 mg/ml) $425 
Anti-MCL1 / MCL 1 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Immunofluorescent staining of HepG2 cells using anti-MCL1 mouse monoclonal antibody.
Anti-MCL1 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MCL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCL1.
HEK293T cells transfected with either pCMV6-ENTRY MCL1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-MCL1 mouse monoclonal, and then analyzed by flow cytometry.
Flow cytometry of Jurkat cells, using anti-MCL1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-MCL1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Anti-MCL1 / MCL 1 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Immunofluorescent staining of HepG2 cells using anti-MCL1 mouse monoclonal antibody.
Anti-MCL1 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MCL1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCL1.
HEK293T cells transfected with either pCMV6-ENTRY MCL1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-MCL1 mouse monoclonal, and then analyzed by flow cytometry.
Flow cytometry of Jurkat cells, using anti-MCL1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-MCL1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
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IHCPlus™ MCL1 / MCL 1 Antibody Monoclonal Flo,IF,IHC,WB LS‑B10210

Note: This antibody replaces LS-C115036
Antibody:
Mouse Monoclonal to Human MCL1 / MCL 1
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B10210-50
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human MCL1 / MCL 1
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
MCL 1 antibody LS-B10210 is an unconjugated mouse monoclonal antibody to human MCL 1 (MCL1 ). Validated for Flow, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human MCL1 / MCL 1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Clone
2E11
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50) & (10 µg/ml)
  • Immunofluorescence (1:100)
  • Western blot (1:2000)
  • Flow Cytometry (1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Full length human recombinant protein of human MCL1 (NP_068779) produced in HEK293T cell.
Specificity
Human MCL 1
Usage
Concentration: 0.5-1.0 mg/ml (Lot dependent)
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MCL1 / MCL 1
Q07820 NM_021960 NP_068779.1

Validation

Anti-MCL1 / MCL 1 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IHC Validated
Anti-MCL1 / MCL 1 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-MCL1 / MCL 1 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

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Requested From: United States
Date Requested: 9/23/2019