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IHCPlus™ MCL1 / MCL 1 Antibody Monoclonal ELISA,IF,IHC,WB LS‑B1583

Catalog Number Size Price
LS-B1583-50 50 µl (1 mg/ml) $425 
Anti-MCL1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Confocal immunofluorescence of HepG2 cells using MCL1 mouse monoclonal antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Western blot using MCL1 mouse monoclonal antibody against HeLa (1), BCBL-1 (2), Jurkat (3) and HL60 (4) cell lysate.
Anti-MCL1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Confocal immunofluorescence of HepG2 cells using MCL1 mouse monoclonal antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Western blot using MCL1 mouse monoclonal antibody against HeLa (1), BCBL-1 (2), Jurkat (3) and HL60 (4) cell lysate.
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IHCPlus™ MCL1 / MCL 1 Antibody Monoclonal ELISA,IF,IHC,WB LS‑B1583

Note: This antibody replaces LS-C41385, LS-C355301
Antibody:
Mouse Monoclonal to Human MCL1 / MCL 1
Application:
IHC, IHC-P, IHC-Fr, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B1583-50
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human MCL1 / MCL 1
Application:
IHC, IHC-P, IHC-Fr, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
MCL 1 antibody LS-B1583 is an unconjugated mouse monoclonal antibody to human MCL 1 (MCL1 ). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human MCL1 / MCL 1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
8C6D4B1
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:200 - 1:1000)
  • IHC - Frozen (1:200 - 1:1000)
  • Immunofluorescence (1:200 - 1:1000)
  • Western blot (1:100 - 1:500)
  • ELISA (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Purified recombinant fragment of human MCL-1 expressed in E. coli.
Specificity
Ni-NTA purified recombinant protein MCL-1expressed in E. Coli strain M15
Usage
Immunohistochemistry: LS-B1583 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1583 was determined to be 1:200-1:1000.
Presentation
PBS, 0.03% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MCL1 / MCL 1
Q07820 NM_021960 NP_068779.1

Validation

Anti-MCL1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
IHC Validated
Anti-MCL1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-MCL1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

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Requested From: United States
Date Requested: 9/17/2019