Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
MCL1 / MCL 1 antibody was raised against human Mcl-1 synthetic peptide (amino acid residues 121-139), conjugated to KLH.
This immunoaffinity purified antibody Detects a ~42/43kD protein doublet, corresponding to the expected molecular mass of Mcl-1 on SDS-PAGE immunoblots, in samples from human, canine, guinea pig, monkey, porcine and rabbit. The antibody specificity is confirmed by peptide inhibition studies. An additional protein at ~33kD may also be detected. Other additional proteins may also be seen in some samples.
Western Blot (ECL): 0.1 ug/mL. Positive control: HeLa Heat Shocked Cell Lysate.
25 mM Sodium Borate, BBS, pH 8.2, 75 mM NaCl 5 mM EDTA, 100 mM Boric acid
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Myeloid cell leukimia-1 (Mcl-1) is a member of the Bcl-2 family of proteins that can act to promote cell survival. While the mechanism by which Mcl-1 inhibits apoptosis is not known, it is thought that it may heterodimerize and neutralize pro-apoptotic members of the Bcl-2 family such as Bim or Bak. Mcl-1 was originally identified in differentiating myeloid cells, but has since been shown to be expressed in multiple cell types.