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Anti-Leptin Antibody LS-C104760

Note: This antibody replaces LS-C54168, LS-C25189

Ordering

Wt. Vol. Conc. Price
50 µg - - Unavailable
100 µg - - Unavailable

Related Products

216 Leptin Antibodies

Most Popular Leptin Antibodies

Anti-Leptin Antibody (N-Terminus) IHC-plus™ LS-B4651
Rabbit Polyclonal (IgG) to Human Leptin
Human, Mouse, Bovine, Dog, Hamster, Horse, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-Leptin Antibody (C-Terminus) IHC-plus™ LS-B4652
Rabbit Polyclonal to Human Leptin
Human, Monkey, Mouse, Rat, Bovine, Dog, Goat, Hamster, Horse, Sheep, Chicken
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-Leptin Antibody IHC-plus™ LS-B8192
Mouse Monoclonal (IgG1,k) to Human Leptin
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-Leptin Antibody (clone 3G1-1C9) LS-C197610
Mouse Monoclonal [clone 3G1-1C9] (IgG2b,k) to Human Leptin
Human
Western blot, ELISA
Unconjugated
Enzyme-Linked Immunosorbent Assay Image
Anti-Leptin Antibody (aa8-37, PE) LS-C261830
Rabbit Polyclonal (IgG) to Human Leptin
Human
IHC, ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
PE Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Mouse Leptin
Mouse
IHC - 4% paraformaldehyde, Western blot, ELISA (applications tested for the base form of this product only)
Unconjugated

Details

Mouse Leptin
Rabbit
Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin.
Immunoaffinity purified
Unmodified

Applications

  • IHC - 4% paraformaldehyde (0.2 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA
  • (applications tested for the base form of this product only)

Specificity and Use

Leptin antibody was raised against e.coli derived recombinant Murine Leptin.
Mouse Leptin
Immunohistochemistry: This antibody stained 4% PFA, paraffin-embedded sections of Trichuris muris infected murine tissue. The primary antibody was incubated at 0.20 ug/mL overnight at 4°C and the secondary antibody was a cyanine-3 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100mM citric acid was used. Information and photo are courtesy of David Artis, University of Pennsylvania. Optimal concentrations and conditions may vary. ELISA: To detect mLeptin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine Leptin (LS-C104759) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mLeptin. Western Blot: To detect mLeptin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mLeptin is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Packaging

Lyophilized from PBS, pH 7.2
Reconstitute with 50ul sterile ddH2O.
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About Leptin

P41159 NM_000230 NP_000221.1

LEP Antibody, Leptin Antibody, LEPD Antibody, Obese protein Antibody, Obese, mouse, homolog of Antibody, Obesity factor Antibody, OBS Antibody, OB Antibody

Leptin is a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing.

Immunohistochemistry

Immunohistochemistry
IHC of Leptin antibody LS-C104760. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
IHC of Leptin antibody LS-C104760. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western Blot (reducing) of Leptin antibody LS-C104760. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western Blot (non-reducing) of Leptin antibody LS-C104760. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of Leptin antibody LS-C104760. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 4/24/2017

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