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Anti-Leptin Antibody LS-C104645

Note: This antibody replaces LS-C54170, LS-C25158, LS-C187759


Wt. Vol. Conc. Price
50 µg - - $285
100 µg - - $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular Leptin Antibodies

Anti-Leptin Antibody (N-Terminus) IHC-plus™ LS-B4651
Rabbit Polyclonal (IgG) to Human Leptin
Human, Mouse, Bovine, Dog, Hamster, Horse, Rabbit
IHC - Paraffin, Western blot
Immunohistochemistry Image
Anti-Leptin Antibody (C-Terminus) IHC-plus™ LS-B4652
Rabbit Polyclonal to Human Leptin
Human, Monkey, Mouse, Rat, Bovine, Dog, Goat, Hamster, Horse, Sheep, Chicken
IHC - Paraffin, Western blot
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human Leptin
IHC - Paraffin, Western blot, ELISA


Human Leptin
Human (tested or 100% immunogen sequence identity)
Immunoaffinity purified


  • IHC - Paraffin (0.5 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)

Specificity and Use

Leptin antibody was raised against e.coli derived recombinant Human Leptin.
Human Leptin
Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.5ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. ELISA: To detect hLeptin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Leptin as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hLeptin. Western Blot: To detect hLeptin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLeptin is 1.5-3 ng/lane, under either reducing or non-reducing conditions.


Lyophilized from PBS, pH 7.2
Sterile water
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About Leptin

P41159 NM_000230 NP_000221.1

LEP Antibody, Leptin Antibody, LEPD Antibody, Obese protein Antibody, Obese, mouse, homolog of Antibody, Obesity factor Antibody, OBS Antibody, OB Antibody

Leptin is a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing.


IHC of Leptin antibody LS-C104645


IHC of Leptin antibody LS-C104645

Western blot

Western blot
Western Blot (non-reducing) of Leptin antibody LS-C104645

Western blot

Western blot
Western Blot (reducing) of Leptin antibody LS-C104645

Western blot

Western blot
Western Blot (non-reducing) of Leptin antibody LS-C104645

Western blot

Western blot
Western Blot (reducing) of Leptin antibody LS-C104645

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of Leptin antibody LS-C104645

Requested From: United States
Date Requested: 10/22/2016

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