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Anti-HSPA5 / GRP78 / BiP Antibody IHC-plus™ LS-B3388

Note: This antibody replaces LS-C98698
Catalog Size Price
LS-B3388-200 200 µl Unavailable

Most Popular HSPA5 / GRP78 / BiP Antibodies

Anti-HSPA5 / GRP78 / BiP Antibody IHC-plus™ LS-B3388
Rabbit Polyclonal to Human HSPA5 / GRP78 / BiP
Human, Mouse
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Anti-HSPA5 / GRP78 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3388 concentration 10 ug/ml.
Anti-HSPA5 / GRP78 / BiP Antibody (aa525-628, clone 4000) IHC-plus™ LS-B4157
Mouse Monoclonal [clone 4000] (IgG1) to Human HSPA5 / GRP78 / BiP
Human
IHC, IHC - Paraffin, Western blot, ELISA
Unconjugated
Anti-HSPA5 / GRP78 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4157 dilution 1:200.
Anti-HSPA5 / GRP78 / BiP Antibody (aa541-568) LS-C165063
Rabbit Polyclonal to Human HSPA5 / GRP78 / BiP
Human
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
HSPA5 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human colon carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
Anti-HSPA5 / GRP78 / BiP Antibody (aa261-289) LS-C165064
Rabbit Polyclonal to Human HSPA5 / GRP78 / BiP
Human, Zebrafish
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated
HSPA5 Antibody IHC of formalin-fixed and paraffin-embedded human lung carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
Anti-HSPA5 / GRP78 / BiP Antibody (Gly584) IHC-plus™ LS-B9789
Rabbit Monoclonal (IgG) to Human HSPA5 / GRP78 / BiP
Human, Mouse
IHC, IHC - Paraffin, IHC - Frozen, Western blot
Unconjugated
Anti-HSPA5 / GRP78 / BIP antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

100% Guaranteed
Rabbit Polyclonal to Human HSPA5 / GRP78 / BiP
Human, Mouse
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human HSPA5 / GRP78 / BiP
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

Specificity and Use

Immunohistochemistry: LS-B3388 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3388 was determined to be 10 ug/ml.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About HSPA5 / GRP78 / BiP

P11021 M19645 AAA52614.1

HSPA5 Antibody, BIP Antibody, Heat shock 70 kDa protein 5 Antibody, MIF2 Antibody, GRP-78 Antibody, GRP78 Antibody

Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.

Immunohistochemistry

Anti-HSPA5 / GRP78 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3388 concentration 10 ug/ml.
Anti-HSPA5 / GRP78 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3388 concentration 10 ug/ml.

Immunohistochemistry

Anti-HSPA5 / GRP78 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3388 concentration 10 ug/ml.
Anti-HSPA5 / GRP78 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3388 concentration 10 ug/ml.

Immunofluorescence

Confocal immunofluorescence of HSPA5 Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of HSPA5 Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of anti-HSPA5 antibody in HL60 cell line lysates (35 ug/lane). HSPA5(arrow) was detected using the purified antibody.
Western blot of anti-HSPA5 antibody in HL60 cell line lysates (35 ug/lane). HSPA5(arrow) was detected using the purified antibody.

Western blot

The anti-HSPA5 antibody is used in Western blot to detect HSPA5 in mouse liver tissue lysate. HSPA5 (arrow) was detected using the purified antibody.
The anti-HSPA5 antibody is used in Western blot to detect HSPA5 in mouse liver tissue lysate. HSPA5 (arrow) was detected using the purified antibody.

Flow Cytometry

HSPA5 Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
HSPA5 Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: 
Date Requested: 5/24/2018

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