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Catalog Number Size Price
LS-B17000-50 50 µg (0.5 mg/ml) $460 
HSPA5 / GRP78 / BiP Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPA5 / GRP78 / BiP Antibody - Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPA5 / GRP78 / BiP Antibody - Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPA5 / GRP78 / BiP Antibody - HSPA5 / GRP78 / BiP antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
HSPA5 / GRP78 / BiP Antibody - HSPA5 / GRP78 / BiP antibody (1µg/ml) staining of HepG2 (A) and MCF7 (B) lysates (35µg protein in RIPA buffer).Detected by chemiluminescence.
HSPA5 / GRP78 / BiP Antibody - HSPA5 / GRP78 / BiP antibody (0.3µg/ml) staining of Mouse Liver (A) and Testes (B) and (0.5ug/ml) Rat Brain (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
HSPA5 / GRP78 / BiP Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPA5 / GRP78 / BiP Antibody - Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPA5 / GRP78 / BiP Antibody - Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPA5 / GRP78 / BiP Antibody - HSPA5 / GRP78 / BiP antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
HSPA5 / GRP78 / BiP Antibody - HSPA5 / GRP78 / BiP antibody (1µg/ml) staining of HepG2 (A) and MCF7 (B) lysates (35µg protein in RIPA buffer).Detected by chemiluminescence.
HSPA5 / GRP78 / BiP Antibody - HSPA5 / GRP78 / BiP antibody (0.3µg/ml) staining of Mouse Liver (A) and Testes (B) and (0.5ug/ml) Rat Brain (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
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IHC‑plus™ Polyclonal Goat anti‑Human HSPA5 / GRP78 / BiP Antibody (IHC, IF, WB) LS‑B17000

IHC‑plus™ Polyclonal Goat anti‑Human HSPA5 / GRP78 / BiP Antibody (IHC, IF, WB) LS‑B17000

Note: This antibody replaces LS-C502373
Antibody:
HSPA5 / GRP78 / BiP Goat anti-Human Polyclonal Antibody
Application:
IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B17000-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
HSPA5 / GRP78 / BiP Goat anti-Human Polyclonal Antibody
Application:
IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
BiP antibody LS-B17000 is an unconjugated goat polyclonal antibody to human BiP (HSPA5 / GRP78). Validated for IF, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human HSPA5 / GRP78 / BiP
Synonyms
HSPA5 | BIP | Heat shock 70 kDa protein 5 | MIF2 | GRP-78 | GRP78
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-ESHQDADIEDFK, from the internal region (near C-Terminus) of the protein sequence according to NP_005338.1.
Applications
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (5 µg/ml)
  • Western blot (0.3 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSPA5 / GRP78 / BiP
P11021 M19645 AAA52614.1

Validation

HSPA5 / GRP78 / BiP Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

HSPA5 / GRP78 / BiP Antibody - Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

HSPA5 / GRP78 / BiP Antibody - Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ HSPA5 / GRP78 / BiP Antibody for IHC, IF/Immunofluorescence, WB/Western LS-B17000 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/27/2024