Products
Research Areas
COVID-19
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, Proforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-B13122-50 50 µl (1 mg/ml) $440 
HSPA5 / GRP78 / BiP Antibody - Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPA5 / GRP78 / BiP Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPA5 / GRP78 / BiP Antibody - Immunofluorescent analysis of GRP78 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
HSPA5 / GRP78 / BiP Antibody - Western blot analysis of GRP78 expression in HepG2 (A); HeLa (B); Raw264.7 (C); mouse brain (D) whole cell lysates.
HSPA5 / GRP78 / BiP Antibody - Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPA5 / GRP78 / BiP Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPA5 / GRP78 / BiP Antibody - Immunofluorescent analysis of GRP78 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
HSPA5 / GRP78 / BiP Antibody - Western blot analysis of GRP78 expression in HepG2 (A); HeLa (B); Raw264.7 (C); mouse brain (D) whole cell lysates.
1 of 4
2 of 4
3 of 4
4 of 4

IHC‑plus™ Polyclonal Rabbit anti‑Human HSPA5 / GRP78 / BiP Antibody (C‑Terminus, IHC, IF, WB) LS‑B13122

IHC‑plus™ Polyclonal Rabbit anti‑Human HSPA5 / GRP78 / BiP Antibody (C‑Terminus, IHC, IF, WB) LS‑B13122

Note: This antibody replaces LS-C352339
Antibody:
HSPA5 / GRP78 / BiP Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human, Monkey, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$440
LS-B13122-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
HSPA5 / GRP78 / BiP Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human, Monkey, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
BiP antibody LS-B13122 is an unconjugated rabbit polyclonal antibody to BiP (HSPA5 / GRP78) (C-Terminus) from human. It is reactive with human, mouse and monkey. Validated for ICC, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human HSPA5 / GRP78 / BiP
Synonyms
HSPA5 | BIP | Heat shock 70 kDa protein 5 | MIF2 | GRP-78 | GRP78
Host
Rabbit
Reactivity
Human, Monkey, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human GRP78.
Epitope
C-Terminus
Specificity
Recognizes endogenous levels of GRP78 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation (1:10 - 1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSPA5 / GRP78 / BiP
P11021 M19645 AAA52614.1

Validation

HSPA5 / GRP78 / BiP Antibody - Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)

HSPA5 / GRP78 / BiP Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ HSPA5 / GRP78 / BiP Antibody (C-Terminus) for IHC, ICC, IF/Immunofluorescence, WB/Western, IP LS-B13122 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/4/2021