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Catalog Number Size Price
LS-C15345-50 50 µg (1 mg/ml) $582 
Antibody

Monoclonal Mouse anti‑Rat HSPA5 / GRP78 / BiP Antibody (clone 10C3, aa649‑654, IHC, WB) LS‑C15345

Monoclonal Mouse anti‑Rat HSPA5 / GRP78 / BiP Antibody (clone 10C3, aa649‑654, IHC, WB) LS‑C15345

Antibody:
HSPA5 / GRP78 / BiP Mouse anti-Rat Monoclonal (aa649-654) (10C3) Antibody
Application:
IHC, ICC, WB, IP, ELISA
Reactivity:
Rat, Yeast
Format:
Unconjugated, Unmodified
Price
Catalog Number
$582
LS-C15345-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
HSPA5 / GRP78 / BiP Mouse anti-Rat Monoclonal (aa649-654) (10C3) Antibody
Application:
IHC, ICC, WB, IP, ELISA
Reactivity:
Rat, Yeast
Format:
Unconjugated, Unmodified

Specifications

Description
BiP antibody LS-C15345 is an unconjugated mouse monoclonal antibody to BiP (HSPA5 / GRP78) (aa649-654) from rat. It is reactive with rat and yeast. Validated for ELISA, ICC, IHC, IP and WB.
Target
Rat HSPA5 / GRP78 / BiP
Synonyms
HSPA5 | BIP | Heat shock 70 kDa protein 5 | MIF2 | GRP-78 | GRP78
Host
Mouse
Reactivity
Rat, Yeast (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Clone
10C3
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
A 6 residue synthetic peptide, SEKDEL, based on the rat Grp78 (aa 649-654), and the peptide coupled to KLH.
Epitope
aa649-654
Specificity
Identifies purified Grp78 (BiP) protein and reacts with proteins corresponding to the apparent molecular mass of Grp78 and Grp94 on SDS-PAGE immunoblots in samples from mammal, bird, insect and plant origin. Cross reacts with yeast proteins containing the HDEL sequence. An unidentified protein at ~55kD is also detected in some lysates. This antibody does not react with Hsp70 (Hsp72) or Hsc70 (Hsp73).
Applications
  • IHC (1:400)
  • ICC (5 µg/ml)
  • Western blot
  • Immunoprecipitation (5 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Western Blot (Colorimetric): 4 ug/mL. Immunoprecipitation: 5 ug/mL. Immunocytochemistry: 5 ug/mL. Immunohistochemistry: 1:400. Positive control: HeLa Heat Shocked Cell Lysate.
Presentation
PBS, pH 7.2, 0.09% Sodium Azide, 50% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSPA5 / GRP78 / BiP
P11021 M19645 AAA52614.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/18/2024