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Catalog Number Size Price
LS-B2038-50 50 µg (0.5 mg/ml) $460 
HSPA5 / GRP78 / BiP Antibody - Anti-HSPA5 / GRP78 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
HSPA5 / GRP78 / BiP Antibody - Western blot of GRP78 in mouse liver tissue enriched for endoplasmic reticulum, and 1 ug/ml of HSPA5 / GRP78 / BiP Antibody and 0.25 ug/ml of anti-GAPDH loading control. Lane A contains 20 ugs of whole mouse liver lysate, lane B contains 20 ugs of total ER fraction, and lane C contains 20 ugs of rough ER fraction. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution, were used for this test.
HSPA5 / GRP78 / BiP Antibody - Western blot of BiP in HeLa cell lysate using antibody at 1 ug/ml.
HSPA5 / GRP78 / BiP Antibody - Anti-HSPA5 / GRP78 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
HSPA5 / GRP78 / BiP Antibody - Western blot of GRP78 in mouse liver tissue enriched for endoplasmic reticulum, and 1 ug/ml of HSPA5 / GRP78 / BiP Antibody and 0.25 ug/ml of anti-GAPDH loading control. Lane A contains 20 ugs of whole mouse liver lysate, lane B contains 20 ugs of total ER fraction, and lane C contains 20 ugs of rough ER fraction. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution, were used for this test.
HSPA5 / GRP78 / BiP Antibody - Western blot of BiP in HeLa cell lysate using antibody at 1 ug/ml.
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IHC‑plus™ Polyclonal Rabbit anti‑Human HSPA5 / GRP78 / BiP Antibody (aa550‑600, IHC, WB) LS‑B2038

IHC‑plus™ Polyclonal Rabbit anti‑Human HSPA5 / GRP78 / BiP Antibody (aa550‑600, IHC, WB) LS‑B2038

Note: This antibody replaces LS-C637
Antibody:
HSPA5 / GRP78 / BiP Rabbit anti-Human Polyclonal (aa550-600) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B2038-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
HSPA5 / GRP78 / BiP Rabbit anti-Human Polyclonal (aa550-600) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
BiP antibody LS-B2038 is an unconjugated rabbit polyclonal antibody to human BiP (HSPA5 / GRP78) (aa550-600). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human HSPA5 / GRP78 / BiP
Synonyms
HSPA5 | BIP | Heat shock 70 kDa protein 5 | MIF2 | GRP-78 | GRP78
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
A portion of amino acids 550-600 of human GRP78.
Epitope
aa550-600
Specificity
A portion of amino acids 550-600 of human BiP was used as the immunogen.
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1 - 3 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B2038 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2038 was determined to be 10 ug/ml.
Presentation
PBS, 0.05% Sodium Azide, 0.05% BSA
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSPA5 / GRP78 / BiP
P11021 M19645 AAA52614.1

Validation

HSPA5 / GRP78 / BiP Antibody - Anti-HSPA5 / GRP78 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-HSPA5 / GRP78 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-HSPA5 / GRP78 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

IHC-plus™ HSPA5 / GRP78 / BiP Antibody (aa550-600) for IHC, WB/Western LS-B2038 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)

Publications (1)

Ultrabright plasmonic fluor nanolabel-enabled detection of a urinary ER stress biomarker in autosomal dominant tubulointerstitial kidney disease. Yeawon Kim, Zheyu Wang, Chuang Li, Kendrah Kidd, Yixuan Wang, Bryce G Johnson, Stanislav Kmoch, Jeremiah J Morrissey, Anthony J Bleyer, Jeremy S Duffield, Srikanth Singamaneni, Ying Maggie Chen. American journal of physiology. Renal physiology. 2021 July;321:F236-F244.

Customer Reviews (0)


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Requested From: United States
Date Requested: 5/23/2024