Human HIF1A / HIF1 Alpha

Rabbit

Human, Monkey, Mouse, Rat, Bovine, Dog, Xenopus (tested or 100% immunogen sequence identity)

Monkey, Mouse, Rat, Bovine, Dog, Xenopus (at least 90% immunogen sequence identity)

Polyclonal

Unconjugated

Antiserum

Unmodified

This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region surrounding the P564 of human HIF-1a.

This antibody is directed against human HIF-1a hydroxyP564 and is specific for the hydroxylated form of the protein. Minimal reactivity occurs with the non-hydroxylated form of the protein. A BLAST analysis was used to suggest cross-reactivity with HIF-1a from human, monkey, mouse, rat, dog, bovine and Xenopus sources based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

• IHC
• Western blot (1:500 - 1:2000)
• ELISA (1:5000 - 1:20000)

This antibody has been tested for use in ELISA and western blotting. This antibody is specific for HIF-1a hydroxylated at P564. Minimal cross-reactivity occurs with non-hydroxylated HIF-1a. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 110 kD in size corresponding to HIF-1a HYDROXYP564 by western blotting in the appropriate cell lysate or extract.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Store at 4°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q16665 NCBI: NM_001530 NP_001521.1