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Anti-HIF1A / HIF1 Alpha Antibody (aa530-825, HRP) LS-C121233
Most Popular HIF1A / HIF1 Alpha Antibodies
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Details
| Mouse HIF1A / HIF1 Alpha | |
| Rabbit | |
| Mouse, Human, Rat, Primate (tested or 100% immunogen sequence identity) | |
| Polyclonal | |
| Horseradish Peroxidase | |
| Immunoaffinity purified | |
| Unmodified |
Applications
- IHC - Paraffin (5 - 10 µg/ml)
- Western blot (1:500 - 1:2000)
Specificity and Use
| HIF1A / HIF1 Alpha antibody was raised against a fusion protein including aa530-825 of the mouse HIF-1 alpha protein. | |
| aa530-825 | |
| Recognizes mouse HIF-1 alpha. Species cross-reactivity: rat, human, primate. Antibody has been tested in Western Blot, recognizing a band in hypoxic samples at ~115kD. Rat retina and brain cortex have been tested. Rat brain cortex is a bit dirty but this antibody does detect upregulation of HIF-1 alpha in hypoxic samples. | |
| Suitable for Western Blot and Immunohistochemistry. Not recommended for Immunoprecipitation. Western Blot: 1:500-1:2000. Immunohistochemistry (regular/paraffin): 5-10 ug/ml. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. |
Packaging
| With 0.05% sodium azide. Labeled, HRP | |
| Store at 2-8°C. Do Not Freeze! Aliquots are stable for at least 3 months. Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates | |
| For research use only. |
About HIF1A / HIF1 Alpha
Q16665
NM_001530
NP_001521.1
HIF1A Antibody, ARNT-interacting protein Antibody, ARNT interacting protein Antibody, BHLHe78 Antibody, HIF1 Alpha Antibody, Hypoxia-inducible factor1alpha Antibody, HIF-1A Antibody, HIF1 Antibody, MOP1 Antibody, Member of PAS superfamily 1 Antibody, PASD8 Antibody, HIF-1-alpha Antibody, HIF-1alpha Antibody, HIF1-ALPHA Antibody, Member of PAS protein 1 Antibody
Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease.
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Date Requested: 6/27/2017
Date Requested: 6/27/2017
