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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GFP Antibody (N‑Terminus) LS‑C129445
Antibody
GFP antibody LS-C129445 is an unconjugated mouse monoclonal antibody to aequorea victoria GFP. Validated for ELISA, IP and WB.
Catalog
Size
Price
LS-C129445-100
100 µg (1 mg/ml)
Unavailable

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Species: Aequorea victoria
Applications: Western blot, Immunoprecipitation
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Species: Aequorea victoria
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Antibody
Species: Aequorea victoria
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Anti-GFP Chemiluminescent Kit for Western blot. Known amounts of recombinant GFP protein ( were spiked into a HeLa cell-derived lysates (p/n W09-000-364) and separated by SDS-PAGE using a 4-20% gradient gel. Proteins were transferred onto a nitrocellulose membrane for 1 h at 100 mV. The membrane was blocked with TTBS (p/n MB-013) supplemented with 1% BSA (p/n BSA-50) for 1 h at 4°C prior to probing the blot with the anti-GFP monoclonal antibody LS-C154208 diluted 1:1000 for 40 min. Detection of the primary antibody by the HRP-conjugated anti-Mouse IgG (p/n LS-C60772) was performed at a dilution of 1:20000 for 1h at 4°C. FemtoMax Super Sensitive Chemiluminescent Luminol Substrate was used for signal detection (see below).
Species: Aequorea victoria
Applications: IHC, IHC - Frozen, Immunofluorescence, Western blot, ELISA

Product Description

GFP antibody LS-C129445 is an unconjugated mouse monoclonal antibody to aequorea victoria GFP. Validated for ELISA, IP and WB.
About GFP
Green Fluorescent Protein (GFP) exhibits bright green fluorescence when exposed to blue light. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. P42212 M62653 P42212

GFP Antibody


Specifications

Target
Aequorea victoria GFP
Host
Mouse
Reactivity
Aequorea victoria (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
  • Western blot (1:1000)
  • Immunoprecipitation
  • ELISA
Immunogen
GFP antibody was raised against gFP from the jellyfish Aequorea Victoria N-terminal peptide (KLH).
Epitope
N-Terminus
Specificity
Recognizes native and denatured forms of GFP and its variants EGFP, YFP, EYFP, and CFP.
Usage
Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Dot Blot. Western Blot: 1:1000.
Presentation
10 mM PBS, pH 7.2, 0.09% sodium azide.
Storage
May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/23/2018

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