Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Green Fluorescent Protein (GFP) exhibits bright green fluorescence when exposed to blue light. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum.
Lane 1-3: 200 ng, 40 ng, 8 ng GFP fusion protein Detection antibody: Mouse Anti-cGFP-tag Monoclonal Antibody LS-C51080 The Western blot was performed using One-Step Western Basic Kit with 4 ug of the antibody added to 4 ml WB solution.
Specificity analysis of THETM GFP Antibody, mAb, Mouse LS-C51080 by Western blot of a variety of variants of GFP protein such as eGFP, cGFP and AcGFP1. The signal was developed with IRDye 800 Conjugated Goat Anti-mouse IgG.
Lane 1-3: 100 ng, 25 ng, 10 ng GFP fusion protein Lane 4: Negative control Primary antibody: A. 1 ug/ml Mouse Anti-cGFP-tag Monoclonal Antibody LS-C51080 B. 1 ug/ml Mouse Anti-cGFP-tag Monoclonal Antibody (X Company) Secondary antibody: Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody
Lane 1: OFP transfecting 293 cell lysate Lane 2: EGFP transfecting 293 cell lysate Lane 3: 5 ng GFPuv protein Lane 4: Negative 293 cell lysate Primary antibody: A. 1 ug/ml Mouse Anti-cGFP-tag Monoclonal Antibody LS-C51080 B. 1 ug/ml Mouse Anti-cGFP-tag Monoclonal Antibody (X Company) Secondary antibody: Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody