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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CLU / Clusterin Antibody (aa44‑58) IHC‑plus™ LS‑B14366
Note: This antibody replaces LS-C155049
Clusterin antibody LS-B14366 is an unconjugated goat polyclonal antibody to human Clusterin (CLU). Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B14366-50
50 µg (0.5 mg/ml)
Unavailable

Popular CLU / Clusterin Products

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Anti-Clusterin antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2852 concentration 5 ug/ml.
Species: Human, Monkey
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Antibody
Species: Human
Applications: ELISA

Product Description

Clusterin antibody LS-B14366 is an unconjugated goat polyclonal antibody to human Clusterin (CLU). Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
About CLU / Clusterin
Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. P10909 NM_001831 NP_001822.3

CLU Antibody, APO-J Antibody, Apolipoprotein J Antibody, CLU1 Antibody, Clusterin Antibody, Complement lysis inhibitor Antibody, CLI Antibody, AAG4 Antibody, Aging-associated protein 4 Antibody, Ku70-binding protein 1 Antibody, KUB1 Antibody, SGP2 Antibody, APOJ Antibody, SGP-2 Antibody, Sulfated glycoprotein 2 Antibody, CLU2 Antibody, Complement cytolysis inhibitor Antibody, NA1/NA2 Antibody, SP-40 Antibody


Specifications

Target
Human CLU / Clusterin
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey, Hamster (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.03 - 0.1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CLU / Clusterin antibody was raised against synthetic peptide C-KEIQNAVNGVKQIKT from an internal region of human Clusterin (NP_976084.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon (100%); Monkey, Marmoset, Hamster (93%); Galago, Mouse, Rat (87%); Panda, Dog, Guinea pig (80%).
Blocking Peptide
LS-E27943 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B14366. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa44-58
Specificity
Human Clusterin.
Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 37kD band observed in human Prostate lysates (calculated MW of 52.5 according to NP_976084.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (PMID: 9713325 and PMID: 11560857). Recommended concentration: 0.03-0.1 ug/ml.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Immunohistochemistry - Paraffin

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry - Paraffin

Human Liver, Portal Triad: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver, Portal Triad: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

CLU antibody (0.03 ug/ml) staining of Human Prostate lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
CLU antibody (0.03 ug/ml) staining of Human Prostate lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry - Paraffin

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry - Paraffin

Human Liver, Portal Triad: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver, Portal Triad: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

CLU antibody (0.03 ug/ml) staining of Human Prostate lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
CLU antibody (0.03 ug/ml) staining of Human Prostate lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry - Paraffin

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry - Paraffin

Human Liver, Portal Triad: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver, Portal Triad: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

CLU antibody (0.03 ug/ml) staining of Human Prostate lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
CLU antibody (0.03 ug/ml) staining of Human Prostate lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry - Paraffin

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry - Paraffin

Human Liver, Portal Triad: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver, Portal Triad: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

CLU antibody (0.03 ug/ml) staining of Human Prostate lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
CLU antibody (0.03 ug/ml) staining of Human Prostate lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: 
Date Requested: 7/17/2018

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