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Catalog Number Size Price
LS-B2852-50 50 µg (0.5 mg/ml) $440 
CLU / Clusterin Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
CLU / Clusterin Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
CLU / Clusterin Antibody - CLU / Clusterin antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
CLU / Clusterin Antibody - CLU / Clusterin antibody (0.1µg/ml) staining of Human Colon lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
CLU / Clusterin Antibody - Antibody (0.3 ug/ml) staining of human colon lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
CLU / Clusterin Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
CLU / Clusterin Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
CLU / Clusterin Antibody - CLU / Clusterin antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
CLU / Clusterin Antibody - CLU / Clusterin antibody (0.1µg/ml) staining of Human Colon lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
CLU / Clusterin Antibody - Antibody (0.3 ug/ml) staining of human colon lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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IHC‑plus™ Polyclonal Goat anti‑Human CLU / Clusterin Antibody (aa488‑501, IHC, IF, WB) LS‑B2852

IHC‑plus™ Polyclonal Goat anti‑Human CLU / Clusterin Antibody (aa488‑501, IHC, IF, WB) LS‑B2852

Note: This antibody replaces LS-C54661, LS-C79452
Antibody:
CLU / Clusterin Goat anti-Human Polyclonal (aa488-501) Antibody
Application:
IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Price
Catalog Number
$440
LS-B2852-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
CLU / Clusterin Goat anti-Human Polyclonal (aa488-501) Antibody
Application:
IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified

Specifications

Description
Clusterin antibody LS-B2852 is an unconjugated goat polyclonal antibody to Clusterin (CLU) (aa488-501) from human. It is reactive with human and monkey. Validated for IF, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CLU / Clusterin
Synonyms
CLU | APO-J | Apolipoprotein J | CLU1 | Clusterin | Complement lysis inhibitor | CLI | AAG4 | Aging-associated protein 4 | Ku70-binding protein 1 | KUB1 | SGP2 | APOJ | SGP-2 | Sulfated glycoprotein 2 | CLU2 | Complement cytolysis inhibitor | NA1/NA2 | SP-40
Host
Goat
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-EKALQEYRKKHREE, from the C-Terminus of protein sequence according to NP_001822.2NP_976084.1.
Epitope
aa488-501
Specificity
Human CLU / Clusterin. This antibody is expected to recognise both reported isoforms (NP_001822.2 and NP_976084.1)
Applications
  • IHC - Paraffin (2.5 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot (0.03 - 0.1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B2852 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2852 was determined to be 2.5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CLU / Clusterin
P10909 NM_001831 NP_001822.3

Validation

CLU / Clusterin Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

CLU / Clusterin Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ CLU / Clusterin Antibody (aa488-501) for IHC, IF/Immunofluorescence, WB/Western LS-B2852 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/23/2022