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Anti-CFLAR / FLIP Antibody (aa206-222) IHC-plus™ LS-B2024

Note: This antibody replaces LS-C716, LS-C77924

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CFLAR / FLIP Antibodies

Anti-CFLAR / FLIP Antibody (aa191-209) IHC-plus™ LS-B3187
Rabbit Polyclonal to Human CFLAR / FLIP
Human, Mouse
IHC - Paraffin, ICC, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CFLAR / FLIP Antibody (clone 2F12) IHC-plus™ LS-B5753
Mouse Monoclonal [clone 2F12] (IgG2a,k) to Human CFLAR / FLIP
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CFLAR / FLIP Antibody (aa1-480, clone NF6) LS-C344945
Mouse Monoclonal [clone NF6] (IgG1) to Human CFLAR / FLIP
Human
IHC - Paraffin, ICC, Western blot
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human CFLAR / FLIP
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human CFLAR / FLIP
Rabbit
Human (tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot

Specificity and Use

CFLAR / FLIP antibody was raised against a peptide corresponding to amino acids 206-222 GRSKEQRLKEQLGAQQE of human I-FLICE.
aa206-222
A peptide corresponding to the aa. 206-222 GRSKEQRLKEQLGAQQE of human I-FLICE was used to immunize rabbits. Anti-I-FLICE antibody was purified by passing the immune serum through peptide column.
Immunohistochemistry: LS-B2024 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2024 was determined to be 10 ug/ml.

Packaging

PBS, 0.05% BSA, 0.05% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About CFLAR / FLIP

O15519 NM_003879 NP_003870.4

CFLAR Antibody, C-FLIPR Antibody, C-FLIPS Antibody, C-FLIP Antibody, CASP8AP1 Antibody, Cflip Antibody, CLARP Antibody, CASH Antibody, Caspase homolog Antibody, Caspase-eight-related protein Antibody, Flice inhibitory protein Antibody, I-FLICE Antibody, Inhibitor of FLICE Antibody, FLAME Antibody, FLAME1 Antibody, MRIT Antibody, Usurpin beta Antibody, C-FLIPL Antibody, Casper Antibody, FLAME-1 Antibody, FLIP Antibody, Usurpin Antibody

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively.

Immunohistochemistry

Immunohistochemistry
Anti-CFLAR / FLIP antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2024 concentration 10 ug/ml.

Western blot

Western blot
Western blot of I-FLICE/ CASPER using antibody at 2 ug/ml against 15 ug of HeLa cell lysate.

Requested From: United States
Date Requested: 12/10/2016

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