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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C148270-50 50 µl $335 
CFLAR / FLIP Antibody - IHC of FLIP in tissues cores from formalin-fixed, paraffin-embedded human brain tumor microarray using Polyclonal Antibody to FLIP (long and short) at 1:2000. A-C, tissue cores from a patient with gemistocytoma. A, normal adjacent brain tissue. Astrocytes are positive for Flip expression. B, gemistocytoma (Grade II). Gemistocytes are positive for Flip expression. C, peritumoural edema brain tissue. Reactive astrocytes are positive for FLIP expression. C1, a higher magnification of C. Collectively, the data shows that astrocytes (normal), reactive astrocytes and gemistocytes are positive for Flip expression. Hematoxylin-Eos in counterstain.
CFLAR / FLIP Antibody - IHC of FLIP in two tissues cores from a formalin-fixed, paraffin-embedded human brain tumor microarray using Polyclonal Antibody to FLIP (long and short) at 1:2000. A, gemistocytoma (Grade II) positive for FLIP expression. B, gemistocytoma (Grade II) negative for FLIP expression. A1 and A2 are higher magnifications from A. A, A1, and A2 show expression of FLIP in the gemistocytes of the tumor. Hematoxylin-Eosin counterstain.
CFLAR / FLIP Antibody - IHC of FLIP in tissues cores from formalin-fixed, paraffin-embedded human brain tumor microarray using Polyclonal Antibody to FLIP (long and short) at 1:2000. A-C, tissue cores from a patient with gemistocytoma. A, normal adjacent brain tissue. Astrocytes are positive for Flip expression. B, gemistocytoma (Grade II). Gemistocytes are positive for Flip expression. C, peritumoural edema brain tissue. Reactive astrocytes are positive for FLIP expression. C1, a higher magnification of C. Collectively, the data shows that astrocytes (normal), reactive astrocytes and gemistocytes are positive for Flip expression. Hematoxylin-Eos in counterstain.
CFLAR / FLIP Antibody - IHC of FLIP in two tissues cores from a formalin-fixed, paraffin-embedded human brain tumor microarray using Polyclonal Antibody to FLIP (long and short) at 1:2000. A, gemistocytoma (Grade II) positive for FLIP expression. B, gemistocytoma (Grade II) negative for FLIP expression. A1 and A2 are higher magnifications from A. A, A1, and A2 show expression of FLIP in the gemistocytes of the tumor. Hematoxylin-Eosin counterstain.
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CFLAR / FLIP Antibody (aa75‑92) LS‑C148270

CFLAR / FLIP Antibody (aa75‑92) LS‑C148270

Antibody:
Rabbit Polyclonal to Human CFLAR / FLIP
Application:
IHC, IHC-P, IHC-Fr, WB, IP
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Price
Catalog Number
$335 
LS-C148270-50
50 µl
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human CFLAR / FLIP
Application:
IHC, IHC-P, IHC-Fr, WB, IP
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified

Specifications

Description
FLIP antibody LS-C148270 is an unconjugated rabbit polyclonal antibody to FLIP (CFLAR ) from human and monkey. Validated for IHC, IP and WB.
Target
Human CFLAR / FLIP
Host
Rabbit
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:1000 - 1:5000)
  • IHC - Frozen
  • Western blot (1:1000 - 1:2000)
  • Immunoprecipitation (1:50 - 1:200)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A synthetic peptide corresponding to amino acids 75-92 (DRKAVETHLLRNPHLVS) of human FLIP, GenBank no. gi|12643547. This amino acid sequence is 100% conserved in FLIP long and short, and various FLIP isoforms as denoted in the NCBI BLAST data base. http://www. ncbi. nlm. nih. gov/blast /. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey (100%); Gibbon (94%); Marmoset (88%); Panda (82%); Mouse (76%); Rat (71%).
Epitope
aa75-92
Specificity
The gene encoding FLIP produces, by alternative splicing, 24 different transcripts, all with introns, putatively encoding 22 different protein isoforms. The molecular weights of these isoforms may vary. Therefore, the molecular weight of FLIP observed by western blot may vary according to the isoforms present in the sample.
Presentation
Antiserum, 0.05% sodium azide
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CFLAR / FLIP
O15519 NM_003879 NP_003870.4

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Requested From: United States
Date Requested: 11/17/2019