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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C274-0.1 0.1 mg (1 mg/ml) $375 
CFLAR / FLIP Antibody - Immunohistochemistry of FLIP in mouse liver tissue with FLIP antibody at 5 µg/mL.
CFLAR / FLIP Antibody - Immunocytochemistry of FLIP in HeLa cells with FLIP antibody at 5 ug/ml.
CFLAR / FLIP Antibody - Immunofluorescence of FLIP in mouse liver tissue with FLIP antibody at 20 µg/ml.Red: FLIP Antibody  Blue: DAPI staining
CFLAR / FLIP Antibody - Immunofluorescence of FLIP in HeLa cells with FLIP antibody at 20 ug/ml.
CFLAR / FLIP Antibody - Western blot of FLIP in K562 cell lysate with FLIP antibody at (A) 1 and (B) 2 ug/ml.
CFLAR / FLIP Antibody - Immunohistochemistry of FLIP in mouse liver tissue with FLIP antibody at 5 µg/mL.
CFLAR / FLIP Antibody - Immunocytochemistry of FLIP in HeLa cells with FLIP antibody at 5 ug/ml.
CFLAR / FLIP Antibody - Immunofluorescence of FLIP in mouse liver tissue with FLIP antibody at 20 µg/ml.Red: FLIP Antibody  Blue: DAPI staining
CFLAR / FLIP Antibody - Immunofluorescence of FLIP in HeLa cells with FLIP antibody at 20 ug/ml.
CFLAR / FLIP Antibody - Western blot of FLIP in K562 cell lysate with FLIP antibody at (A) 1 and (B) 2 ug/ml.
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Polyclonal Rabbit anti‑Human CFLAR / FLIP Antibody (IHC, IF, WB) LS‑C274

Polyclonal Rabbit anti‑Human CFLAR / FLIP Antibody (IHC, IF, WB) LS‑C274

Antibody:
Rabbit Polyclonal to Human CFLAR / FLIP
Application:
IHC, ICC, IF, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$375 
LS-C274-0.1
0.1 mg
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human CFLAR / FLIP
Application:
IHC, ICC, IF, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
FLIP antibody LS-C274 is an unconjugated rabbit polyclonal antibody to FLIP (CFLAR) from human. It is reactive with human, mouse and rat. Validated for ELISA, Flow, ICC, IF, IHC and WB.
Target
Human CFLAR / FLIP
Synonyms
CFLAR | C-FLIPR | C-FLIPS | C-FLIP | CASP8AP1 | Cflip | CLARP | CASH | Caspase homolog | Caspase-eight-related protein | Flice inhibitory protein | I-FLICE | Inhibitor of FLICE | FLAME | FLAME1 | MRIT | Usurpin beta | C-FLIPL | Casper | FLAME-1 | FLIP | Usurpin
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Applications
  • IHC
  • ICC
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
  • Flow Cytometry
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A peptide corresponding to 17 amino acids near the N-Terminus of human FLIP. The sequence is identical in all FLIP splice variants. The immunogen is located within the first 50 amino acids of FLIP.
Blocking Peptide
LS-E29858 - Liquid - 0.05 mg 200 µg/ml - $145.00
Immunizing peptide used to generate LS-C274. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Specificity
FLIP recognizes all FLIP splice variants including FLIPa, b, and y.
Usage
FLIP antibody can be used for Western blot at 1 - 2 ug/mL. Antibody can also be used for immunocytochemistry starting at 5 ug/mL and immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested. Western Blot: Predicted: 53 kDa Observed: 53 kDa
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CFLAR / FLIP
O15519 NM_003879 NP_003870.4

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Requested From: United States
Date Requested: 6/2/2020