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Catalog Number	Size	Price
LS-C756465-10	10 µg	$318
LS-C756465-100	100 µg	$470

CFLAR / FLIP Antibody - IHC analysis of FLIP using anti-FLIP antibody. FLIP was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2?g/ml rabbit anti-FLIP Antibody overnight at 4?C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

CFLAR / FLIP Antibody - IHC analysis of FLIP using anti-FLIP antibody. FLIP was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2?g/ml rabbit anti-FLIP Antibody overnight at 4?C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

CFLAR / FLIP Antibody - Western blot analysis of FLIP using anti-FLIP antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates,Lane 2: human placenta tissue lysates, Lane 3: human COLO-320 whole cell lysates,Lane 4: human PANC-1 whole cell lysates,Lane 5: human HepG2 whole cell lysates,Lane 6: human MDA-MB-231 whole cell lysates,Lane 7: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FLIP antigen affinity purified polyclonal antibody at 0.5 ?g/mL overnight at 4?C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FLIP at approximately 43KD. The expected band size for FLIP is at 55KD.

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Polyclonal Rabbit anti‑Human CFLAR / FLIP Antibody (IHC, WB) LS‑C756465

Name: Anti-CFLAR / FLIP Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C756465-10
Price: 318.0000 USD
Availability: InStock

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Antibody:

CFLAR / FLIP Rabbit anti-Human Polyclonal Antibody

Application:

IHC-P, WB, ELISA

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

$318

LS-C756465-10

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

CFLAR / FLIP Rabbit anti-Human Polyclonal Antibody

Application:

IHC-P, WB, ELISA

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

FLIP antibody LS-C756465 is an unconjugated rabbit polyclonal antibody to FLIP (CFLAR) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB.

Target

Human CFLAR / FLIP

Synonyms

See All CFLAR / FLIP Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

IgG Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

E. coli-derived human FLIP recombinant protein (Position: M1-D376).

Specificity

No cross reactivity with other proteins.

Applications

IHC - Paraffin (0.5 - 1 µg/ml)
Western blot (0.1 - 0.5 µg/ml)
ELISA (0.1 - 0.5 µg/ml)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Applications should be user optimized.

Presentation

Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.