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Antibodies > CFLAR / FLIP > LS-C375460
 
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Catalog Number Size Price
LS-C375460-50 50 µg $294 
LS-C375460-100 100 µg $360 
CFLAR / FLIP Antibody - Immunohistochemistry of paraffin-embedded human adrenal gland tissue using antibody at dilution of 1:100.
CFLAR / FLIP Antibody - Immunohistochemistry of paraffin-embedded human colon cancer using antibody at dilution of 1:100.
CFLAR / FLIP Antibody - IHC image of CFLAR Antibody diluted at 1:400 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
CFLAR / FLIP Antibody - IHC image of CFLAR Antibody diluted at 1:400 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
CFLAR / FLIP Antibody - Immunofluorescence staining of Hela cells with CFLAR Antibody at 1:40, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
CFLAR / FLIP Antibody - Western Blot Positive WB detected in: Jurkat whole cell lysate, K562 whole cell lysate, 293T whole cell lysate All lanes: CFLAR antibody at 4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 56, 26, 42, 28, 52, 51, 40, 31, 35, 53, 34, 24, 50, 45 kDa Observed band size: 56 kDa
CFLAR / FLIP Antibody - Western blot. All lanes: CFLAR antibody at 1.2 ug/ml. Lane 1: HeLa whole cell lysate. Lane 2: A431 whole cell lysate. Secondary Goat polyclonal to Rabbit IgG at 1:10000 dilution. Predicted band size: 55 kDa. Observed band size: 55 kDa.
CFLAR / FLIP Antibody - Immunohistochemistry of paraffin-embedded human adrenal gland tissue using antibody at dilution of 1:100.
CFLAR / FLIP Antibody - Immunohistochemistry of paraffin-embedded human colon cancer using antibody at dilution of 1:100.
CFLAR / FLIP Antibody - IHC image of CFLAR Antibody diluted at 1:400 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
CFLAR / FLIP Antibody - IHC image of CFLAR Antibody diluted at 1:400 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
CFLAR / FLIP Antibody - Immunofluorescence staining of Hela cells with CFLAR Antibody at 1:40, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
CFLAR / FLIP Antibody - Western Blot Positive WB detected in: Jurkat whole cell lysate, K562 whole cell lysate, 293T whole cell lysate All lanes: CFLAR antibody at 4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 56, 26, 42, 28, 52, 51, 40, 31, 35, 53, 34, 24, 50, 45 kDa Observed band size: 56 kDa
CFLAR / FLIP Antibody - Western blot. All lanes: CFLAR antibody at 1.2 ug/ml. Lane 1: HeLa whole cell lysate. Lane 2: A431 whole cell lysate. Secondary Goat polyclonal to Rabbit IgG at 1:10000 dilution. Predicted band size: 55 kDa. Observed band size: 55 kDa.
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Polyclonal Rabbit anti‑Human CFLAR / FLIP Antibody (IHC, WB) LS‑C375460

Polyclonal Rabbit anti‑Human CFLAR / FLIP Antibody (IHC, WB) LS‑C375460

Submit Review LSBio Guarantee
Antibody:
CFLAR / FLIP Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
HRP Biotin FITC
Price
Catalog Number
$294
LS-C375460-50
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CFLAR / FLIP Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
HRP Biotin FITC

Specifications

Description
FLIP antibody LS-C375460 is an unconjugated rabbit polyclonal antibody to human FLIP (CFLAR). Validated for ELISA, IHC and WB.
Target
Human CFLAR / FLIP
Synonyms
CFLAR | C-FLIPR | C-FLIPS | C-FLIP | CASP8AP1 | Cflip | CLARP | CASH | Caspase homolog | Caspase-eight-related protein | Flice inhibitory protein | I-FLICE | Inhibitor of FLICE | FLAME | FLAME1 | MRIT | Usurpin beta | C-FLIPL | Casper | FLAME-1 | FLIP | Usurpin
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with HRP, Biotin, FITC.
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Recombinant human CASP8 and FADD-like apoptosis regulator protein.
Specificity
Human CFLAR / FLIP
Applications
  • IHC
  • IHC - Paraffin (1:20 - 1:200)
  • Western blot (1:500 - 1:2000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Storage
Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CFLAR / FLIP
O15519 NM_003879 NP_003870.4

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IHC‑plus™ CFLAR / FLIP Antibody (clone 2F12) LS‑B5753
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
CFLAR / FLIP Antibody - Anti-CFLAR / FLIP antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ CFLAR / FLIP Antibody LS‑B3187
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
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Species: Human
Applications: IHC, IHC - Paraffin, ICC, Western blot
CFLAR / FLIP Antibody - Cell lysates of mouse kidney, MCF7 and rat spleen (40 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human FLIP (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
CFLAR / FLIP Antibody (aa1‑376, clone 5D8) LS‑C93734
Species: Human
Applications: Western blot, ELISA
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CFLAR / FLIP Antibody (PE) LS‑C652146
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CFLAR / FLIP Antibody - Immunocytochemistry of FLIP in 3T3 cells with FLIP antibody at 5 ug/ml.
CFLAR / FLIP Antibody LS‑C272
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See all CFLAR / FLIP Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/27/2024
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