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Catalog Number Size Price
LS-C357582-10 10 µg $318 
LS-C357582-100 100 µg (0.5 mg/ml) $470 
p66 / SHC Antibody - SHC1 antibody IHC-paraffin: Human Lung Cancer Tissue.
p66 / SHC Antibody - SHC1 antibody IHC-paraffin: Rat Brain Tissue.
p66 / SHC Antibody - SHC1 antibody IHC-paraffin: Mouse Brain Tissue.
p66 / SHC Antibody - anti-SHC1 Picoband antibody IHC(F)IHC(F): Human Placenta Tissue
p66 / SHC Antibody - Western blot analysis of SHC using anti-SHC antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysate, Lane 2: human Hela whole cell lysate, Lane 3: human HepG2 whole cell lysate, Lane 4: human Jurkat whole cell lysate, Lane 5: human K562 whole cell lysate, Lane 6: human THP-1 whole cell lysate, Lane 7: rat C6 whole cell lysate, Lane 8: mouse thymus tissue lysate, Lane 9: mouse RAW246. 7 whole cell lysate, Lane 10: mouse NIH3T3 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHC antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. Specific bands were detected for SHC at approximately 46, 52, 66KD. The expected band size for SHC are at 46, 52, 66KD.
p66 / SHC Antibody - SHC1 antibody Western blot. All lanes: Anti SHC1 at 0.5 ug/ml. Lane 1: Rat Thymus Tissue Lysate at 50 ug. Lane 2: Mouse Testis Tissue Lysate at 50 ug. Lane 3: JURKAT Whole Cell Lysate at 40 ug. Lane 4: K562 Whole Cell Lysate at 40 ug. Lane 5: HELA Whole Cell Lysate at 40 ug. Predicted band size: 63 kD. Observed band size: 63 kD.
p66 / SHC Antibody - SHC1 antibody IHC-paraffin: Human Lung Cancer Tissue.
p66 / SHC Antibody - SHC1 antibody IHC-paraffin: Rat Brain Tissue.
p66 / SHC Antibody - SHC1 antibody IHC-paraffin: Mouse Brain Tissue.
p66 / SHC Antibody - anti-SHC1 Picoband antibody IHC(F)IHC(F): Human Placenta Tissue
p66 / SHC Antibody - Western blot analysis of SHC using anti-SHC antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysate, Lane 2: human Hela whole cell lysate, Lane 3: human HepG2 whole cell lysate, Lane 4: human Jurkat whole cell lysate, Lane 5: human K562 whole cell lysate, Lane 6: human THP-1 whole cell lysate, Lane 7: rat C6 whole cell lysate, Lane 8: mouse thymus tissue lysate, Lane 9: mouse RAW246. 7 whole cell lysate, Lane 10: mouse NIH3T3 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHC antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. Specific bands were detected for SHC at approximately 46, 52, 66KD. The expected band size for SHC are at 46, 52, 66KD.
p66 / SHC Antibody - SHC1 antibody Western blot. All lanes: Anti SHC1 at 0.5 ug/ml. Lane 1: Rat Thymus Tissue Lysate at 50 ug. Lane 2: Mouse Testis Tissue Lysate at 50 ug. Lane 3: JURKAT Whole Cell Lysate at 40 ug. Lane 4: K562 Whole Cell Lysate at 40 ug. Lane 5: HELA Whole Cell Lysate at 40 ug. Predicted band size: 63 kD. Observed band size: 63 kD.
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Polyclonal Rabbit anti‑Human p66 / SHC Antibody (aa536‑564, IHC, WB) LS‑C357582

Polyclonal Rabbit anti‑Human p66 / SHC Antibody (aa536‑564, IHC, WB) LS‑C357582

Antibody:
p66 / SHC Rabbit anti-Human Polyclonal (aa536-564) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Chimpanzee, Monkey, Mouse, Rat, Bat, Horse, Pig, Sheep
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C357582-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
p66 / SHC Rabbit anti-Human Polyclonal (aa536-564) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Chimpanzee, Monkey, Mouse, Rat, Bat, Horse, Pig, Sheep
Format:
Unconjugated, Unmodified

Specifications

Description
SHC antibody LS-C357582 is an unconjugated rabbit polyclonal antibody to SHC (p66) (aa536-564) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB.
Target
Human p66 / SHC
Synonyms
SHC1 | p46 shc | p52 shc | SH2 domain protein C1 | p66 | SHCA | SHC | SHC-transforming protein 1 | SHC-transforming protein 3 | SHC-transforming protein A
Host
Rabbit
Reactivity
Human, Chimpanzee, Monkey, Mouse, Rat, Bat, Horse, Pig, Sheep (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human SHC (536-564 aa VDPEGVVRTKDHRFESVSHLISYHMDNHL), identical to the related mouse and rat sequences.
Epitope
aa536-564
Specificity
Widely expressed. Expressed in neural stem cells but absent in mature neurons.
Applications
  • IHC
  • IHC - Paraffin (0.1 - 0.5 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About p66 / SHC
P29353 NM_003029 NP_003020.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/18/2024