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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CDKN1A / WAF1 / p21

cyclin-dependent kinase inhibitor 1A (p21, Cip1)

CDKN1A / WAF1 / p21 is a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Multiple alternatively spliced variants have been found for this gene.

Gene Name: cyclin-dependent kinase inhibitor 1A (p21, Cip1)
Synonyms: CDKN1A, CDKN1, CIP1, CDK-interacting protein 1, DNA synthesis inhibitor, p21CIP1, MDA6, PIC1, SDI1, WAF1, CAP20, CDK-interaction protein 1, MDA-6, p21
Target Sequences: NM_078467 NP_000380.1 P38936

Publications (8)

1
The p21 cyclin-dependent kinase inhibitor suppresses tumorigenicity in vivo. Yang ZY, Perkins ND, Ohno T, Nabel EG, Nabel GJ. Nature medicine. 1995 1:1052-6. [PubMed:7489362] Related Antibodies: LS-C59514.
2
p21 is necessary for the p53-mediated G1 arrest in human cancer cells. Waldman T, Kinzler KW, Vogelstein B. Cancer research. 1995 55:5187-90. [PubMed:7585571] Related Antibodies: LS-C59514.
3
A cis-acting element in Rous sarcoma virus long terminal repeat required for promoter repression by HeLa nuclear protein p21. Yeh CH, Shatkin AJ. The Journal of biological chemistry. 1995 270:15815-20. [PubMed:7797584] Related Antibodies: LS-C59514.
4
p53 expression overcomes p21WAF1/CIP1-mediated G1 arrest and induces apoptosis in human cancer cells. Kagawa S, Fujiwara T, Hizuta A, Yasuda T, Zhang WW, Roth JA, Tanaka N. Oncogene. 1997 15:1903-9. [PubMed:9365236] Related Antibodies: LS-C59514.
5
Inhibition of tumor cell growth by p21WAF1 adenoviral gene transfer in lung cancer. Joshi US, Chen YQ, Kalemkerian GP, Adil MR, Kraut M, Sarkar FH. Cancer gene therapy. 5:183-91. [PubMed:9622102] Related Antibodies: LS-C59514.
6
Inactivation of the retinoblastoma gene yields a mouse model of malignant colorectal cancer. Parisi T, Bronson RT, Lees JA. Oncogene. 2015 34:5890-9. (IP; Mouse) [Full Text Article] [PubMed:25745996] [PMC:PMC4668801]
7
TFIIS.h, a new target of p53, regulates transcription efficiency of pro-apoptotic bax gene. Liao JM, Cao B, Deng J, Zhou X, Strong M, Zeng S, Xiong J, Flemington E, Lu H. Scientific reports. 2016 6:23542. (WB; Human) [Full Text Article] [PubMed:27005522] [PMC:PMC4804275] Related Antibodies: LS-B8634.
8
Expression levels of hnRNP K and p21WAF1/CIP1 are associated with resistance to radiochemotherapy independent of p53 pathway activation in rectal adenocarcinoma. Daskalaki W, Wardelmann E, Port M, Stock K, Steinestel J, Huss S, Sperveslage J, Steinestel K, Eder S. International journal of molecular medicine. 2018 December;42:3269-3277. [Full Text Article] [PubMed:30272263] [PMC:PMC6202084] Related Antibodies: LS-B9242.
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Filtered by: None
Target
CDKN1A / WAF1 / p21 (213)
Reactivity
Human (187)
Mouse (68)
Rat (47)
Bovine (9)
Chimpanzee (7)
Dog (4)
Gibbon (3)
Hamster (4)
Mammal (2)
Monkey (9)
Non-Human Primates (1)
Orangutan (3)
Pig (6)
Sheep (8)
Application
IHC (124)
IHC-Fr (8)
IHC-P (47)
WB (147)
Flo (25)
DB (40)
ELISA (93)
ICC (3)
IF (50)
IP (25)
Peptide-ELISA (2)
Host
rabbit (142)
mouse (70)
chicken (1)
Isotype
IgG (106)
IgG1 (20)
IgG1,k (10)
IgG1,k + IgG2b,k (7)
IgG2a (14)
IgG2a,k (11)
IgG2b (4)
Clonality
monoclonal mc (70)
polyclonal pc (143)
Clone
2F1 (1)
B459 (1)
CIP1/823 (3)
CIP1/823 + DCS-60.2 (3)
CP74 (3)
DCS-60 (1)
DCS-60.2 (10)
HJ21 (5)
HZ52 (1)
OTI2D3 (2)
OTI4B11 (2)
OTI5G1 (2)
OTI6G2 (2)
OTI7C8 (1)
SPM306 (2)
WA1 (1)
WA-1 (7)
WAF21-1 (3)
Format
AP Conjugated (12)
APC (2)
APC Conjugated (14)
Azide-free (20)
Biotin Conjugated (18)
Carrier-free (4)
Concentrated (2)
Cy3 Conjugated (2)
Cy7 Conjugated (2)
FITC Conjugated (16)
HRP Conjugated (16)
PE Conjugated (14)
Preservative Free (2)
Ready-to-Use (8)
Unconjugated (119)
Epitope
aa1-164 (16)
pThr145 (14)
aa117-146 (12)
aa2-164 (9)
aa105-140 (8)
aa134-164 (8)
pSer125 (8)
pSer130 (8)
pSer78 (8)
pThr57 (8)
N-Terminus (4)
C-Terminus (3)
80-160 aa (2)
aa139-164 (2)
Gly139 (1)
Internal (1)
aa111-160 (1)
aa126-154 (1)
aa133-164 (1)
aa58-77 (1)
Publications
No (210)
Yes (3)
Showing 1-25 of 213 results

IHC-plus™ CDKN1A / WAF1 / p21 Antibody (aa111-160) LS-B9242
Publications (1)
Publications (1)
Rabbit Polyclonal (IgG) to Human CDKN1A / WAF1 / p21
Mouse, Rat, Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $425.00
Anti-CDKN1A / WAF1 / p21 antibody IHC staining of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Western blot analysis of lysates from COLO and K562 cells, treated with EGF, using p21 Cip1 Antibody. The lane on the right is blocked with the synthesized peptide.

IHC-plus™ CDKN1A / WAF1 / p21 Antibody (clone CP74) LS-B8634
Publications (1)
Publications (1)
Mouse Monoclonal [clone CP74] (IgG2b) to Human CDKN1A / WAF1 / p21
Rat, Human
Flo, IHC, IHC-P, IP, WB
Unconjugated
250µl  $485.00
Anti-CDKN1A / WAF1 / p21 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.

CDKN1A / WAF1 / p21 Antibody LS-C136937
Rabbit Polyclonal (IgG) to Human CDKN1A / WAF1 / p21
Human
ELISA, IHC, WB
Unconjugated
100µl  $275.00
Immunohistochemistry Image
Western blot Image

CDKN1A / WAF1 / p21 Antibody LS-C408480
Rabbit Polyclonal (IgG) to Human CDKN1A / WAF1 / p21
Mouse, Rat, Human
IF, IHC, IHC-P, WB
Unconjugated
50µl  $275.00; 100µl  $355.00; 200µl  $455.00
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human SmallIntestine: Formalin-Fixed, Paraffin-Embedded (FFPE)

CDKN1A / WAF1 / p21 Antibody (clone WA-1) LS-C358002
Mouse Monoclonal [clone WA-1] (IgG1,k) to Human CDKN1A / WAF1 / p21
Mouse, Rat, Human, Monkey
Flo, IF, IHC, IHC-P
Unconjugated
20µg 0.2mg/ml  $295.00; 100µg 0.2mg/ml  $465.00
p21 antibody WA-1 immunohistochemistry.  This image was taken for the unmodified form of this product. Other forms have not been tested.

CDKN1A / WAF1 / p21 Antibody (clone WA-1) LS-C59514
Publications (5)
Publications (5)
Mouse Monoclonal [clone WA-1] to Human CDKN1A / WAF1 / p21
Human
ELISA, IHC, IHC-Fr, IHC-P, WB
Unconjugated
100µg 1mg/ml  $425.00
Anti-Human p21WAF1 Monoclonal Antibody - Western Blot. Mab anti-Human p21WAF1 antibody (clone WA-1) is shown to detect human p21 by western blot. Detection occurs after 10 ug of a HeLa whole cell lysate is loaded per lane. The blot was incubated with a 1:1000 dilution of Mab anti-Human p21WAF1at room temperature for 30 min followed by detection using IRDye800 labeled Goat-a-Mouse IgG [H&L] ( diluted 1:5000. A single band corresponding to human p21WAF1 is detected at ~21 kD when compared with known molecular weight standards (not shown). The antibody may be used to detect endogenous human p21WAF1. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

CDKN1A / WAF1 / p21 Antibody (clone DCS-60.2) LS-C389945
Mouse Monoclonal [clone DCS-60.2] (IgG2a,k) to Human CDKN1A / WAF1 / p21
Human
Flo, IF, IHC, IHC-P, WB
Unconjugated
20µg 0.2mg/ml  $295.00; 100µg 0.2mg/ml  $465.00
Formalin-fixed, paraffin-embedded human colon carcinoma stained with p21 antibody (DCS-60.2).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Western blot testing of human MCF7 cell lysate with p21 antibody (clone DCS-60.2).

CDKN1A / WAF1 / p21 Antibody (clone DCS-60.2, Ready-to-Use) LS-C389942
Mouse Monoclonal [clone DCS-60.2] (IgG2a,k) to Human CDKN1A / WAF1 / p21
Human
Flo, IF, IHC, IHC-P, WB
Unconjugated, Ready-to-Use
7ml  $465.00
Formalin-fixed, paraffin-embedded human colon carcinoma stained with p21 antibody (DCS-60.2).  This image was taken for the unmodified form of this product. Other forms have not been tested.

CDKN1A / WAF1 / p21 Antibody (clone HJ21) LS-C389956
Mouse Monoclonal [clone HJ21] (IgG1,k) to Human CDKN1A / WAF1 / p21
Mouse, Rat, Chimpanzee, Human, Monkey
Flo, IF, IHC, IHC-P
Unconjugated
20µg 0.2mg/ml  $295.00; 100µg 0.2mg/ml  $465.00
Formalin-fixed, paraffin-embedded bladder carcinoma stained with p21 antibody (HJ21).  This image was taken for the unmodified form of this product. Other forms have not been tested.

CDKN1A / WAF1 / p21 Antibody (clone WA-1) LS-C45974
Mouse Monoclonal [clone WA-1] (IgG1) to Human CDKN1A / WAF1 / p21
Human, Non-Human Primates
ELISA, ICC, IHC, IHC-P, WB
Unconjugated
0.1mg 1mg/ml  $265.00
Immunohistochemistry staining of colon carcinoma (paraffin-embedded sections) with anti-p21 (WA-1).
Immunofluorescence staining of p21 in human primary fibroblasts using anti-p21 (WA-1; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

CDKN1A / WAF1 / p21 Antibody (clone DCS-60.2) LS-C87964
Mouse Monoclonal [clone DCS-60.2] (IgG2a) to Human CDKN1A / WAF1 / p21
Human
IF, IHC, IHC-P, IP
Unconjugated
100µl 200µg/ml  $285.00; 500µl 200µg/ml  $560.00; 1ml 200µg/ml  $845.00
Formalin-fixed, paraffin-embedded human colon carcinoma stained with peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.  This image was taken for the unmodified form of this product. Other forms have not been tested.

CDKN1A / WAF1 / p21 Antibody LS-C331492
Rabbit Polyclonal (IgG) to Human CDKN1A / WAF1 / p21
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
50µl  $235.00; 100µl  $315.00; 200µl  $425.00
Western blot analysis of extracts of various cell lines, using CDKN1A antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

CDKN1A / WAF1 / p21 Antibody (phospho-Thr145) LS-C117437
Rabbit Polyclonal (IgG) to Human CDKN1A / WAF1 / p21
Mouse, Rat, Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p21 Cip1 (Phospho-Thr145) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from HeLa cells treated with EGF, using p21 Cip1 (Phospho-Thr145) Antibody. The lane on the left is blocked with the phospho peptide.

CDKN1A / WAF1 / p21 Antibody (clone HJ21) LS-C88192
Mouse Monoclonal [clone HJ21] (IgG1) to Human CDKN1A / WAF1 / p21
Rat, Human
IF, IHC-P, WB
Unconjugated
100µl 200µg/ml  $295.00; 500µl 200µg/ml  $590.00; 1ml 200µg/ml  $890.00
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

CDKN1A / WAF1 / p21 Antibody (C-Terminus) LS-C51694
Rabbit Polyclonal to Human CDKN1A / WAF1 / p21
Mouse, Rat, Human
IHC, IP, WB
Unconjugated
200µg 1000µl 0.2mg/ml  $295.00

CDKN1A / WAF1 / p21 Antibody (clone DCS-60.2) LS-C312133
Mouse Monoclonal [clone DCS-60.2] (IgG2a) to Human CDKN1A / WAF1 / p21
Human
IHC, IHC-Fr, IHC-P
Unconjugated
0.1ml  $245.00; 0.5ml  $355.00; 1ml  $465.00
IHC of p21 on FFPE Colon Carcinoma tissue.

CDKN1A / WAF1 / p21 Antibody (N-Terminus) LS-C201205
Rabbit Polyclonal to Human CDKN1A / WAF1 / p21
Sheep, Bovine, Rat, Hamster, Pig, Human
IHC, WB
Unconjugated
100µl 0.5mg/ml  $365.00

CDKN1A / WAF1 / p21 Antibody LS-C387864
Rabbit Polyclonal (IgG) to Human CDKN1A / WAF1 / p21
Mouse, Rat, Human
IHC, WB
Unconjugated
50µl  $235.00; 100µl  $265.00; 200µl  $335.00
Immunohistochemistry of p21 antibody
Western blot of p21 antibody

CDKN1A / WAF1 / p21 Antibody (aa134-164) LS-C99884
Rabbit Polyclonal to Human CDKN1A / WAF1 / p21
Human
Flo, IF, IHC, IHC-P, WB
Unconjugated
400µl  $325.00
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Confocal immunofluorescence of p21 (CDKN1A) Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

CDKN1A / WAF1 / p21 Antibody (clone WA1) LS-C347464
Mouse Monoclonal [clone WA1] (IgG1) to Human CDKN1A / WAF1 / p21
Human, Monkey, Chimpanzee
ELISA, Flo, ICC, IHC, IHC-Fr, IHC-P, WB
Unconjugated
0.1mg  $375.00

CDKN1A / WAF1 / p21 Antibody (phospho-Thr145) LS-C354620
Rabbit Polyclonal to Human CDKN1A / WAF1 / p21
Dog, Sheep, Bovine, Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $265.00
Immunohistochemical analysis of p21 (pT145) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX. w
Immunofluorescent analysis of p21 (pT145) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

CDKN1A / WAF1 / p21 Antibody (phospho-Thr145) LS-C801087
Rabbit Polyclonal (IgG) to Human CDKN1A / WAF1 / p21
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00
Immunohistochemistry Image
Immunofluorescence Image

CDKN1A / WAF1 / p21 Antibody (aa58-77) LS-C17111
Mouse Monoclonal (IgG1,k) to Human CDKN1A / WAF1 / p21
Human
Flo, IF, IHC, IHC-Fr, IHC-P, IP, WB
Unconjugated
50µg  $575.00

CDKN1A / WAF1 / p21 Antibody (clone SPM306) LS-C389943
Mouse Monoclonal [clone SPM306] (IgG2a,k) to Human CDKN1A / WAF1 / p21
Human
Flo, IF, IHC, IHC-P, WB
Unconjugated
20µg 0.2mg/ml  $295.00; 100µg 0.2mg/ml  $465.00
Formalin-fixed, paraffin-embedded human colon carcinoma stained with anti-p21 antibody (SPM306).  This image was taken for the unmodified form of this product. Other forms have not been tested.

CDKN1A / WAF1 / p21 Antibody (clone SPM306, Azide-free) LS-C389964
Mouse Monoclonal [clone SPM306] (IgG2a,k) to Human CDKN1A / WAF1 / p21
Human
Flo, IF, IHC, IHC-P, WB
Unconjugated, Azide-free
100µg 1mg/ml  $465.00
Formalin-fixed, paraffin-embedded human colon carcinoma stained with anti-p21 antibody (SPM306).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Showing 1-25 of 213 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).