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Catalog Number Size Price
LS-C59514-100 100 µg (1 mg/ml) $498 
CDKN1A / WAF1 / p21 Antibody - Anti-Human p21WAF1 Monoclonal Antibody - Western Blot. Mab anti-Human p21WAF1 antibody (clone WA-1) is shown to detect human p21 by western blot. Detection occurs after 10 ug of a HeLa whole cell lysate is loaded per lane. The blot was incubated with a 1:1000 dilution of Mab anti-Human p21WAF1at room temperature for 30 min followed by detection using IRDye800 labeled Goat-a-Mouse IgG [H&L] ( diluted 1:5000. A single band corresponding to human p21WAF1 is detected at ~21 kD when compared with known molecular weight standards (not shown). The antibody may be used to detect endogenous human p21WAF1. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Monoclonal Mouse anti‑Human CDKN1A / WAF1 / p21 Antibody (clone WA‑1, IHC, IF, WB) LS‑C59514

Monoclonal Mouse anti‑Human CDKN1A / WAF1 / p21 Antibody (clone WA‑1, IHC, IF, WB) LS‑C59514

Antibody:
CDKN1A / WAF1 / p21 Mouse anti-Human Monoclonal (WA-1) Antibody
Application:
IHC, IHC-P, IHC-Fr, IF, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$498
LS-C59514-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CDKN1A / WAF1 / p21 Mouse anti-Human Monoclonal (WA-1) Antibody
Application:
IHC, IHC-P, IHC-Fr, IF, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
P21 antibody LS-C59514 is an unconjugated mouse monoclonal antibody to human p21 (CDKN1A / WAF1). Validated for ELISA, IF, IHC, IP and WB. Cited in 5 publications.
Target
Human CDKN1A / WAF1 / p21
Synonyms
CDKN1A | CDKN1 | CIP1 | CDK-interacting protein 1 | DNA synthesis inhibitor | p21CIP1 | MDA6 | PIC1 | SDI1 | WAF1 | CAP20 | CDK-interaction protein 1 | MDA-6 | p21
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
WA-1
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p21 fusion protein.
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • Immunofluorescence (1:250)
  • Western blot (1:2000 - 1:10000)
  • Immunoprecipitation (1:100)
  • ELISA (1:5000 - 1:20000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This antibody is suitable for immunohistochemistry, ELISA and immunoblotting. The antibody detects a 21 kD band corresponding to the p21 protein. MCF7 cells or UV treated fibroblasts are suggested as a positive control for immunoblotting and immunohistochemistry. Methanol/acetone fixed tissue is reactive for immunohistochemistry. The antibody also reacts with breast carcinoma on frozen sections (methanol/acetone fixation) and on paraffin sections (formalin fixation and microwave treatment prior to staining). In ELISA this monoclonal antibody is useful either as a capture antibody (attached to the solid phase) or for detection of p21 protein. This antibody immunoprecipitates monomeric p21 WAF1 and not p21 complexes with other proteins. High level expression of p21 WAF1 requires induction of wild-type p53 expression (e. g. via exposure to DNA damaging agents).
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.5 M NaCl, 0.01% Sodium Azide
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CDKN1A / WAF1 / p21
P38936 NM_078467 NP_000380.1

LSBio Ratings

CDKN1A / WAF1 / p21 Antibody (clone WA-1) for IHC, IF/Immunofluorescence, WB/Western, IP, ELISA LS-C59514 has an LSBio Rating of
Publications (4.4)

Publications (5)

The p21 cyclin-dependent kinase inhibitor suppresses tumorigenicity in vivo. Yang ZY, Perkins ND, Ohno T, Nabel EG, Nabel GJ. Nature medicine. 1995 1:1052-6.

p21 is necessary for the p53-mediated G1 arrest in human cancer cells. Waldman T, Kinzler KW, Vogelstein B. Cancer research. 1995 55:5187-90.

A cis-acting element in Rous sarcoma virus long terminal repeat required for promoter repression by HeLa nuclear protein p21. Yeh CH, Shatkin AJ. The Journal of biological chemistry. 1995 270:15815-20.

p53 expression overcomes p21WAF1/CIP1-mediated G1 arrest and induces apoptosis in human cancer cells. Kagawa S, Fujiwara T, Hizuta A, Yasuda T, Zhang WW, Roth JA, Tanaka N. Oncogene. 1997 15:1903-9.

Inhibition of tumor cell growth by p21WAF1 adenoviral gene transfer in lung cancer. Joshi US, Chen YQ, Kalemkerian GP, Adil MR, Kraut M, Sarkar FH. Cancer gene therapy. 5:183-91.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 8/13/2022