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CDKN1A / WAF1 / p21 Antibody (clone HJ21) LS‑C389956

Catalog Number Size Price
LS-C389956-20 20 µg (0.2 mg/ml) $295 
LS-C389956-100 100 µg (0.2 mg/ml) $465 
Formalin-fixed, paraffin-embedded bladder carcinoma stained with p21 antibody (HJ21).  This image was taken for the unmodified form of this product. Other forms have not been tested.

CDKN1A / WAF1 / p21 Antibody (clone HJ21) LS‑C389956

Antibody:
Mouse Monoclonal to Human CDKN1A / WAF1 / p21
Application:
IHC, IHC-P, IF, Flo
Reactivity:
Human, Chimpanzee, Monkey, Mouse, Rat
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$295 
LS-C389956-20
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human CDKN1A / WAF1 / p21
Application:
IHC, IHC-P, IF, Flo
Reactivity:
Human, Chimpanzee, Monkey, Mouse, Rat
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
p21 antibody LS-C389956 is an unconjugated mouse monoclonal antibody to p21 (CDKN1A / WAF1 ) from human, mouse, rat and other species. Validated for Flow, IF and IHC.
Target
Human CDKN1A / WAF1 / p21
Host
Mouse
Reactivity
Human, Chimpanzee, Monkey, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
HJ21
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified. Also available Azide-free, Ready-to-Use.
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Immunofluorescence (1 - 2 µg/ml)
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Human recombinant protein.
Specificity
Human CDKN1A / WAF1 / p21
Usage
This mAb recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. It is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk s and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma). Optimal dilution of the p21 antibody to be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Presentation
PBS, 0.1mg/ml BSA, 0.05% sodium azide
Storage
Store at 2°C to 8°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CDKN1A / WAF1 / p21
P38936 NM_078467 NP_000380.1

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Requested From: United States
Date Requested: 9/22/2019