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CD274 / B7-H1 / PD-L1

CD274 molecule

CD274, also known as B7-H1 and PD-L1, a cell surface glycoprotein, is a member of the B7 family of co-stimulatory molecules. CD274 is expressed constitutively on macrophages and dendritic cells, and is induced on activated T-cells, B-cells, endothelial cells and epithelial cells in response to interferons. CD274 has dual functions: inhibition of activated effector T cells and co-stimulation of naïve T cells. It inhibits proliferation of activated T cells via ligation to the co-inhibitory molecule CD279 (programmed death-1; PD-1) leading to the secretion of the regulatory cytokine interleukin-10. Expression of CD274 in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma.

Gene Name: CD274 molecule
Synonyms: CD274, B7-H, B7 homolog 1, B7-H1, B7H1, CD274 molecule, CD274 antigen, PDCD1 ligand 1, PDL1, PD-L1, PDCD1L1, PDCD1LG1, Programmed death ligand 1
Target Sequences: NM_014143 NP_054862.1 Q9NZQ7

Publications (19)

1
BRAF inhibition is associated with enhanced melanoma antigen expression and a more favorable tumor microenvironment in patients with metastatic melanoma. Frederick DT, Piris A, Cogdill AP, Cooper ZA, Lezcano C, Ferrone CR, Mitra D, Boni A, Newton LP, Liu C, Peng W, Sullivan RJ, Lawrence DP, Hodi FS, Overwijk WW, Lizée G, Murphy GF, Hwu P, Flaherty KT, Fisher DE, Wargo JA. Clinical cancer research : an official journal of the American Association for Cancer Research. 2013 19:1225-31. (WB; Human) [Full Text Article] [PubMed:23307859] [PMC:PMC3752683]
2
Programmed cell death protein 1 and programmed death-ligand 1 are expressed on the surface of some small-cell lung cancer lines. Yamane H, Isozaki H, Takeyama M, Ochi N, Kudo K, Honda Y, Yamagishi T, Kubo T, Kiura K, Takigawa N. American journal of cancer research. 2015 5:1553-7. (Flo; Human) [PubMed:26101718] [PMC:PMC4473331]
3
Characterization of myeloid leukocytes and soluble mediators in pancreatic cancer: importance of myeloid-derived suppressor cells. Karakhanova S, Link J, Heinrich M, Shevchenko I, Yang Y, Hassenpflug M, Bunge H, von Ahn K, Brecht R, Mathes A, Maier C, Umansky V, Werner J, Bazhin AV. Oncoimmunology. 2015 4:e998519. (Flo; Mouse) [Full Text Article] [PubMed:26137414] [PMC:PMC4485765]
4
PD-1 mediates functional exhaustion of activated NK cells in patients with Kaposi sarcoma. Beldi-Ferchiou A, Lambert M, Dogniaux S, Vély F, Vivier E, Olive D, Dupuy S, Levasseur F, Zucman D, Lebbé C, Sène D, Hivroz C, Caillat-Zucman S. Oncotarget. 2016 7:72961-72977. (WB; Human) [Full Text Article] [PubMed:27662664] [PMC:PMC5341956]
5
EML4-ALK enhances programmed cell death-ligand 1 expression in pulmonary adenocarcinoma via hypoxia-inducible factor (HIF)-1a and STAT3. Koh J, Jang JY, Keam B, Kim S, Kim MY, Go H, Kim TM, Kim DW, Kim CW, Jeon YK, Chung DH. Oncoimmunology. 2016 5:e1108514. (Flo; Human) [Full Text Article] [PubMed:27141364]
6
Induction of PD-L1 Expression by the EML4-ALK Oncoprotein and Downstream Signaling Pathways in Non-Small Cell Lung Cancer. Ota K, Azuma K, Kawahara A, Hattori S, Iwama E, Tanizaki J, Harada T, Matsumoto K, Takayama K, Takamori S, Kage M, Hoshino T, Nakanishi Y, Okamoto I. Clinical cancer research : an official journal of the American Association for Cancer Research. 2015 21:4014-21. (WB; Human) [Full Text Article] [PubMed:26019170]
7
Association of PD-L1 overexpression with activating EGFR mutations in surgically resected nonsmall-cell lung cancer. Azuma K, Ota K, Kawahara A, Hattori S, Iwama E, Harada T, Matsumoto K, Takayama K, Takamori S, Kage M, Hoshino T, Nakanishi Y, Okamoto I. Annals of oncology : official journal of the European Society for Medical Oncology / ESMO. 2014 25:1935-40. (WB; Human) [Full Text Article] [PubMed:25009014]
8
Neuronal programmed cell death-1 ligand expression regulates retinal ganglion cell number in neonatal and adult mice. Sham CW, Chan AM, Kwong JM, Caprioli J, Nusinowitz S, Chen B, Lee JG, Gandhi NM, Francisco LM, Sharpe AH, Chen L, Braun J, Gordon LK. Journal of neuro-ophthalmology : the official journal of the North American Neuro-Ophthalmology Society. 2012 32:227-37. (WB; Human) [Full Text Article] [PubMed:22635166]
9
Clinicopathological and prognostic significance of programmed cell death ligand 1 (PD-L1) expression in patients with esophageal squamous cell carcinoma: a meta-analysis. Qu HX, Zhao LP, Zhan SH, Geng CX, Xu L, Xin YN, Jiang XJ. Journal of thoracic disease. 2016 November;8:3197-3204. (Human) [Full Text Article] [PubMed:28066599] [PMC:PMC5179395]
10
High programmed cell death 1 ligand-1 expression: association with CD8+ T-cell infiltration and poor prognosis in human medulloblastoma. Murata D, Mineharu Y, Arakawa Y, Liu B, Tanji M, Yamaguchi M, Fujimoto KI, Fukui N, Terada Y, Yokogawa R, Yamaguchi M, Minamiguchi S, Miyamoto S. Journal of neurosurgery. 2018 March;128:710-716. [Full Text Article] [PubMed:28474991]
11
Prognostic significance of programmed death ligand-1 immunohistochemical expression in esophageal cancer: A meta-analysis of the literature. Yu W, Guo Y. Medicine. 2018 July;97:e11614. [Full Text Article] [PubMed:30045299] [PMC:PMC6078683]
12
Oncogenic kinase NPM/ALK induces through STAT3 expression of immunosuppressive protein CD274 (PD-L1, B7-H1). Michal Marzec, Qian Zhang, Ami Goradia, Puthiyaveettil N Raghunath, Xiaobin Liu, Michele Paessler, Hong Yi Wang, Maria Wysocka, Mangeng Cheng, Bruce A Ruggeri, Mariusz A Wasik. Proceedings of the National Academy of Sciences of the United States of America. 2008 December;105:20852-7. [Full Text Article] [PubMed:19088198] [PMC:PMC2634900]
13
Juxtacrine Signaling Inhibits Antitumor Immunity by Upregulating PD-L1 Expression. Wen-Hao Yang, Jong-Ho Cha, Weiya Xia, Heng-Huan Lee, Li-Chuan Chan, Ying-Nai Wang, Jennifer L Hsu, Guoxin Ren, Mien-Chie Hung. Cancer research. 2018 July;78:3761-3768. [Full Text Article] [PubMed:29789418] [PMC:PMC6050079] Related Antibodies: LS-C338364.
14
PD-L1-mediated gasdermin C expression switches apoptosis to pyroptosis in cancer cells and facilitates tumour necrosis. Junwei Hou, Rongce Zhao, Weiya Xia, Chiung-Wen Chang, Yun You, Jung-Mao Hsu, Lei Nie, Yeh Chen, Yu-Chuan Wang, Chunxiao Liu, Wei-Jan Wang, Yun Wu, Baozhen Ke, Jennifer L Hsu, Kebin Huang, Zu Ye, Yi Yang, Xianghou Xia, Yintao Li, Chia-Wei Li, Bin Shao, John A Tainer, Mien-Chie Hung. Nature cell biology. 2020 October;22:1264-1275. [Full Text Article] [PubMed:32929201] [PMC:PMC7653546] Related Antibodies: LS-C338364.
15
anlotinib alters tumor immune microenvironment by downregulating PD-L1 expression on vascular endothelial cells. Shaochuan Liu , Tingting Qin , Zhujun Liu, Jing Wang, Yanan Jia, Yingfang Feng, Yuan Gao, Kai Li. Cell death & disease. 2020 May;11:309. [Full Text Article] [PubMed:32366856] [PMC:PMC7198575] Related Antibodies: LS-C746930.
16
Tumor Lymphatic Function Regulates Tumor Inflammatory and Immunosuppressive Microenvironments. Raghu P Kataru, Catherine L Ly, Jinyeon Shin, Hyeung Ju Park, Jung Eun Baik, Sonia Rehal, Sagrario Ortega, David Lyden, Babak J Mehrara. Cancer immunology research. 2019 Aug;7:1345-1358. [Full Text Article] [PubMed:31186247] [PMC:PMC6677612]
17
Metformin Promotes Antitumor Immunity via Endoplasmic-Reticulum-Associated Degradation of PD-L110<sup> Department of Experimental Radiation Oncology, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA12<sup> Department of Breast Surgical Oncology, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA14<sup> Department of Experimental Radiation Oncology, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA; Graduate School of Biomedical Sciences. Jong-Ho Cha, Wen-Hao Yang, Weiya Xia, Yongkun Wei, Li-Chuan Chan, Seung-Oe Lim, Chia-Wei Li, Taewan Kim, Shih-Shin Chang, Heng-Huan Lee, Jennifer L Hsu, Hung-Ling Wang, Chu-Wei Kuo, Wei-Chao Chang, Sirwan Hadad, Colin A Purdie, Aaron M McCoy, Shirong Cai, Yizheng Tu, Jennifer K Litton, Elizabeth A Mittendorf, Stacy L Moulder, William F Symmans, Alastair M Thompson, Helen Piwnica-Worms, Chung-Hsuan Chen, Kay-Hooi Khoo, Mien-Chie Hung. Molecular cell. 2018 Aug;71:606-620.e7. [Full Text Article] [PubMed:30118680] [PMC:PMC6786495] Related Antibodies: LS-C338364.
18
A meta-analysis of CD274 (PD-L1) assessment and prognosis in colorectal cancer and its role in predicting response to anti-PD-1 therapy. Peter G Alexander, Donald C McMillan, James H Park. Critical reviews in oncology/hematology. 2020 November;157:103147. [Full Text Article] [PubMed:33278675] Related Antibodies: LS-C179482.
19
The CD155/TIGIT axis promotes and maintains immune evasion in neoantigen-expressing pancreatic cancer. William A Freed-Pastor, Laurens J Lambert, Zackery A Ely, Nimisha B Pattada, Arjun Bhutkar, George Eng, Kim L Mercer, Ana P Garcia, Lin Lin, William M Rideout rd, William L Hwang, Jason M Schenkel, Alex M Jaeger, Roderick T Bronson, Peter M K Westcott, Tyler D Hether, Prajan Divakar, Jason W Reeves, Vikram Deshpande, Toni Delorey, Devan Phillips, Omer H Yilmaz, Aviv Regev, Tyler Jacks. Cancer cell. 2021 October;39:1342-1360.e14. [Full Text Article] [PubMed:34358448] [PMC:PMC8511341] Related Antibodies: LS-C746930.
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CD274 / B7-H1 / PD-L1 Antibody - Immunohistochemistry of paraffin-embedded rat brain using CD274 Antibody at dilution of 1:100 (40x lens).
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CD274 / B7-H1 / PD-L1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
CD274 / B7-H1 / PD-L1 Antibody - Anti-CD274 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD274.
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CD274 / B7-H1 / PD-L1 Mouse anti-Human Monoclonal (OTI2C7) Antibody
Human
IF, WB
Unconjugated
100 µl/$434
Select
CD274 / B7-H1 / PD-L1 Mouse anti-Human Monoclonal (PE) (27A2) Antibody
Human
Flo
PE Conjugated
1 ml/$430
CD274 / B7-H1 / PD-L1 Antibody - Anti-B7-H1 / PD-L1 / CD274 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
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CD274 / B7-H1 / PD-L1 Rabbit anti-Human Polyclonal (aa261-290) Antibody
Human
IHC, IHC-P, WB
Unconjugated
200 µl/$460
CD274 / B7-H1 / PD-L1 Antibody - Surface staining of mouse splenocytes with anti-mouse B7-H1 (MIH5) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
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CD274 / B7-H1 / PD-L1 Rat anti-Mouse Monoclonal (MIH5) Antibody
Mouse
Flo, IHC, IHC-Fr
Unconjugated
100 µg/$305
CD274 / B7-H1 / PD-L1 Antibody
Select
CD274 / B7-H1 / PD-L1 Mouse anti-Human Monoclonal (27A2) Antibody
Human
Flo, IHC, IHC-P
Unconjugated
100 µg/$430
CD274 / B7-H1 / PD-L1 Antibody - Western blot analysis of extracts of various cell lines, using CD274 antibody.
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CD274 / B7-H1 / PD-L1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
CD274 / B7-H1 / PD-L1 Antibody - Flow cytometry of B7-H1 / PD-L1 / CD274 antibody
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CD274 / B7-H1 / PD-L1 Mouse anti-Human Monoclonal (ANC6H1) Antibody
Human
ELISA, Flo
Unconjugated
100 µg/$446
CD274 / B7-H1 / PD-L1 Antibody - Flow cytometry of B7-H1 / PD-L1 / CD274 antibody This image was taken for the unconjugated form of this product. Other forms have not been tested.
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CD274 / B7-H1 / PD-L1 Mouse anti-Human Monoclonal (Biotin) (ANC6H1) Antibody
Human
ELISA, Flo
Biotin Conjugated
100 µg/$536
CD274 / B7-H1 / PD-L1 Antibody - Flow cytometry of B7-H1 / PD-L1 / CD274 antibody This image was taken for the unmodified form of this product. Other forms have not been tested.
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CD274 / B7-H1 / PD-L1 Mouse anti-Human Monoclonal (Azide-free) (ANC6H1) Antibody
Human
ELISA, Flo
Unconjugated, Azide-free
100 µg/$446
CD274 / B7-H1 / PD-L1 Antibody - IHC of HPV on FFPE LSIL of the Cervix.
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CD274 / B7-H1 / PD-L1 Rabbit anti-Human Recombinant Monoclonal (RBT-PDL1) Antibody
Human
IHC, IHC-Fr, IHC-P
Unconjugated
0.1 ml/$448; 0.5 ml/$941; 1 ml/$1,288
CD274 / B7-H1 / PD-L1 Antibody - Anti-PD L1 Antibody (C-Term) at 1:1000 dilution + Human heart whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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CD274 / B7-H1 / PD-L1 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Human
ELISA, WB
Unconjugated
200 µl/$393
CD274 / B7-H1 / PD-L1 Antibody - Western blot of recombinant B7-H1 / PD-L1 / CD274.
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CD274 / B7-H1 / PD-L1 Rabbit anti-Mouse Polyclonal (aa34-241) Antibody
Mouse
WB
Unconjugated
100 µl/$301; 200 µl/$340; 500 µl/$470; 1 ml/$535; 2 ml/$698; 5 ml/$1,161
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CD274 / B7-H1 / PD-L1 Mouse anti-Human Monoclonal (MIH3) Antibody
Flo
Unconjugated
100 µg/$430
CD274 / B7-H1 / PD-L1 Antibody - Western blot of CD274 / B7-H1 / PD-L1 antibody.
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CD274 / B7-H1 / PD-L1 Rabbit anti-Rat Polyclonal (aa34-241) Antibody
Rat
WB
Unconjugated
100 µl/$306; 200 µl/$347; 500 µl/$484; 1 ml/$553; 2 ml/$737; 5 ml/$1,206
CD274 / B7-H1 / PD-L1 Antibody - Western blot of CD274 / B7-H1 / PD-L1 antibody.
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CD274 / B7-H1 / PD-L1 Rabbit anti-Rat Polyclonal (aa34-241) (Biotin) Antibody
Rat
WB
Biotin Conjugated
100 µl/$316; 200 µl/$361; 500 µl/$511; 1 ml/$587; 2 ml/$809; 5 ml/$1,317
CD274 / B7-H1 / PD-L1 Antibody - Western blot of CD274 / B7-H1 / PD-L1 antibody.
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CD274 / B7-H1 / PD-L1 Rabbit anti-Rat Polyclonal (aa34-241) (PE) Antibody
Rat
WB
PE Conjugated
100 µl/$402; 200 µl/$484; 500 µl/$834; 1 ml/$991; 2 ml/$1,277; 5 ml/$2,395
CD274 / B7-H1 / PD-L1 Antibody - Immunofluorescence of PD-L1 in Human Heart cells with PD-L1 antibody at 20 ug/mL.
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CD274 / B7-H1 / PD-L1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, Flo, IF, WB
Unconjugated
0.1 mg/$460
CD274 / B7-H1 / PD-L1 Antibody - Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Select
Cancer Pathology
Cancer
CD274 / B7-H1 / PD-L1 Rabbit anti-Human Monoclonal (AHC0271) Antibody
Human
IHC-P
Unconjugated
0.05 ml/$440
Select
CD274 / B7-H1 / PD-L1 Mouse anti-Human Monoclonal Antibody
Human
IF, IHC-P, WB
Unconjugated
100 µg/$351
CD274 / B7-H1 / PD-L1 Antibody - Surface staining of CD274 on PHA-stimulated PBMC with anti-CD274 (29E.2A3) PE.
Select
CD274 / B7-H1 / PD-L1 Mouse anti-Human Monoclonal (PE) (29E.2A3) Antibody
Human, Non-Human Primates
Flo, Func, IHC-Fr
PE Conjugated
100 Tests/$365
CD274 / B7-H1 / PD-L1 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD274 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD274.
Select
CD274 / B7-H1 / PD-L1 Mouse anti-Human Monoclonal (OTI4D4) Antibody
Human
WB
Unconjugated
100 µl/$434
CD274 / B7-H1 / PD-L1 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD274 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD274.
Select
CD274 / B7-H1 / PD-L1 Mouse anti-Human Monoclonal (OTI2E3) Antibody
Human
WB
Unconjugated
100 µl/$434
CD274 / B7-H1 / PD-L1 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD274 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD274.
Select
CD274 / B7-H1 / PD-L1 Mouse anti-Human Monoclonal (OTI2B12) Antibody
Human
WB
Unconjugated
100 µl/$434
CD274 / B7-H1 / PD-L1 Antibody
Select
CD274 / B7-H1 / PD-L1 Rat anti-Mouse Monoclonal (APC) (10F.9G2) Antibody
Mouse
Flo
APC Conjugated
50 Tests/$238; 100 Tests/$256; 200 Tests/$278
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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).