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Catalog Number Size Price
LS-C19686-0.1 0.1 mg (1 mg/ml) $420 
CD274 / B7-H1 / PD-L1 Antibody - Immunofluorescence of PD-L1 in Human Heart cells with PD-L1 antibody at 20 ug/mL.
CD274 / B7-H1 / PD-L1 Antibody - Immunofluorescence of PD-L1 in Human Heart cells with PD-L1 antibody at 20 ug/mL.
CD274 / B7-H1 / PD-L1 Antibody - Western blot of PDL-1 in Raji cell lysate with PDL-1 antibody at (A) 0.5 and (B) 1 ug/ml.
CD274 / B7-H1 / PD-L1 Antibody - Flow cytometry analysis of A-20 cells using PD-L1 antibody at 0.5 ug/ml. Green: Isotype control. Red : PD-L1 antibody.
CD274 / B7-H1 / PD-L1 Antibody - Immunofluorescence of PD-L1 in Human Heart cells with PD-L1 antibody at 20 ug/mL.
CD274 / B7-H1 / PD-L1 Antibody - Immunofluorescence of PD-L1 in Human Heart cells with PD-L1 antibody at 20 ug/mL.
CD274 / B7-H1 / PD-L1 Antibody - Western blot of PDL-1 in Raji cell lysate with PDL-1 antibody at (A) 0.5 and (B) 1 ug/ml.
CD274 / B7-H1 / PD-L1 Antibody - Flow cytometry analysis of A-20 cells using PD-L1 antibody at 0.5 ug/ml. Green: Isotype control. Red : PD-L1 antibody.
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Polyclonal Rabbit anti‑Human CD274 / B7‑H1 / PD‑L1 Antibody (IF, WB) LS‑C19686

Polyclonal Rabbit anti‑Human CD274 / B7‑H1 / PD‑L1 Antibody (IF, WB) LS‑C19686

Antibody:
CD274 / B7-H1 / PD-L1 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$420
LS-C19686-0.1
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CD274 / B7-H1 / PD-L1 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
PD-L1 antibody LS-C19686 is an unconjugated rabbit polyclonal antibody to PD-L1 (CD274 / B7-H1) from human. It is reactive with human, mouse and rat. Validated for ELISA, Flow, IF and WB.
Target
Human CD274 / B7-H1 / PD-L1
Synonyms
CD274 | B7-H | B7 homolog 1 | B7-H1 | B7H1 | CD274 molecule | CD274 antigen | PDCD1 ligand 1 | PDL1 | PD-L1 | PDCD1L1 | PDCD1LG1 | Programmed death ligand 1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Immunogen
A 17 AA synthetic peptide near the center of human PD-L1. The immunogen is located within aa 60-110 of PD-L1.
Specificity
PD-L1 antibody has no cross-reactivity to PD-L2.
Applications
  • Immunofluorescence
  • Western blot
  • Flow Cytometry
  • ELISA
Usage
PD-L1 antibody can be used for detection of PD-L1 by Western blot at 0.5 - 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 20 ug/mL. Flow cytometry at 0.5 ug/ml. Antibody validated: Western Blot in human samples; Immunohistochemistry in human and rat samples; Immunofluorescence in human and rat samples and Flow Cytometry in mouse samples. All other applications and species not yet tested. Western Blot: Predicted: 32 kDa Observed: 37 kDa
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD274 / B7-H1 / PD-L1
Q9NZQ7 NM_014143 NP_054862.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/15/2022