Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Locations


Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464


Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers



Tel: +1 (800) 227-6666


Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C746930-20 20 µl (1.66 mg/ml) $275 
LS-C746930-100 100 µl $389 
CD274 / B7-H1 / PD-L1 Antibody - Immunohistochemistry of paraffin-embedded human colon carcinoma using CD274 Antibody at dilution of 1:100 (40x lens).
CD274 / B7-H1 / PD-L1 Antibody - Immunohistochemistry of paraffin-embedded mouse spleen using CD274 Antibody at dilution of 1:100 (40x lens).
CD274 / B7-H1 / PD-L1 Antibody - Immunohistochemistry of paraffin-embedded rat brain using CD274 Antibody at dilution of 1:100 (40x lens).
CD274 / B7-H1 / PD-L1 Antibody - Immunohistochemistry of paraffin-embedded rat spleen using CD274 Antibody at dilution of 1:100 (40x lens).
CD274 / B7-H1 / PD-L1 Antibody - Immunofluorescence analysis of HeLa cells using CD274 antibody. Blue: DAPI for nuclear staining.
CD274 / B7-H1 / PD-L1 Antibody - Western blot analysis of extracts of A-549 cells, using CD274 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
CD274 / B7-H1 / PD-L1 Antibody - Immunohistochemistry of paraffin-embedded human colon carcinoma using CD274 Antibody at dilution of 1:100 (40x lens).
CD274 / B7-H1 / PD-L1 Antibody - Immunohistochemistry of paraffin-embedded mouse spleen using CD274 Antibody at dilution of 1:100 (40x lens).
CD274 / B7-H1 / PD-L1 Antibody - Immunohistochemistry of paraffin-embedded rat brain using CD274 Antibody at dilution of 1:100 (40x lens).
CD274 / B7-H1 / PD-L1 Antibody - Immunohistochemistry of paraffin-embedded rat spleen using CD274 Antibody at dilution of 1:100 (40x lens).
CD274 / B7-H1 / PD-L1 Antibody - Immunofluorescence analysis of HeLa cells using CD274 antibody. Blue: DAPI for nuclear staining.
CD274 / B7-H1 / PD-L1 Antibody - Western blot analysis of extracts of A-549 cells, using CD274 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
1 of 6
2 of 6
3 of 6
4 of 6
5 of 6
6 of 6

Polyclonal Rabbit anti‑Human CD274 / B7‑H1 / PD‑L1 Antibody (IHC, IF, WB) LS‑C746930

Polyclonal Rabbit anti‑Human CD274 / B7‑H1 / PD‑L1 Antibody (IHC, IF, WB) LS‑C746930

Antibody:
CD274 / B7-H1 / PD-L1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C746930-20
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CD274 / B7-H1 / PD-L1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
PD-L1 antibody LS-C746930 is an unconjugated rabbit polyclonal antibody to PD-L1 (CD274 / B7-H1) from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB. Cited in 2 publications.
Target
Human CD274 / B7-H1 / PD-L1
Synonyms
CD274 | B7-H | B7 homolog 1 | B7-H1 | B7H1 | CD274 molecule | CD274 antigen | PDCD1 ligand 1 | PDL1 | PD-L1 | PDCD1L1 | PDCD1LG1 | Programmed death ligand 1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 19-238 of human CD274 (NP_054862.1). FTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNER
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:20 - 1:100)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 20kDa/33kDa, while the observed MW by Western blot was 45kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD274 / B7-H1 / PD-L1
Q9NZQ7 NM_014143 NP_054862.1

LSBio Ratings

CD274 / B7-H1 / PD-L1 Antibody for IHC, IF/Immunofluorescence, WB/Western LS-C746930 has an LSBio Rating of
Publications (4.1)

Publications (2)

anlotinib alters tumor immune microenvironment by downregulating PD-L1 expression on vascular endothelial cells. Shaochuan Liu , Tingting Qin , Zhujun Liu, Jing Wang, Yanan Jia, Yingfang Feng, Yuan Gao, Kai Li. Cell death & disease. 2020 May;11:309.

The CD155/TIGIT axis promotes and maintains immune evasion in neoantigen-expressing pancreatic cancer. William A Freed-Pastor, Laurens J Lambert, Zackery A Ely, Nimisha B Pattada, Arjun Bhutkar, George Eng, Kim L Mercer, Ana P Garcia, Lin Lin, William M Rideout rd, William L Hwang, Jason M Schenkel, Alex M Jaeger, Roderick T Bronson, Peter M K Westcott, Tyler D Hether, Prajan Divakar, Jason W Reeves, Vikram Deshpande, Toni Delorey, Devan Phillips, Omer H Yilmaz, Aviv Regev, Tyler Jacks. Cancer cell. 2021 October;39:1342-1360.e14.

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/13/2024