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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD274 / B7-H1 / PD-L1 Antibody (clone 29E.2A3, PE) LS-C742356

Surface staining of CD274 on PHA-stimulated PBMC with anti-CD274 (29E.2A3) PE.

CD274 / B7-H1 / PD-L1 Antibody (clone 29E.2A3, PE) LS-C742356

Antibody:
Mouse Monoclonal to Human CD274 / B7-H1 / PD-L1
Application:
IHC-Fr, Flo, Func
Reactivity:
Human, Non-Human Primates
Format:
Phycoerythrin, Unmodified
Other formats:
Price
Catalog Number
$315 
LS-C742356-100
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human CD274 / B7-H1 / PD-L1
Application:
IHC-Fr, Flo, Func
Reactivity:
Human, Non-Human Primates
Format:
Phycoerythrin, Unmodified
Other formats:

Specifications

Description
PD-L1 antibody LS-C742356 is a PE-conjugated mouse monoclonal antibody to PD-L1 (CD274 / B7-H1) from human and non-human primates. Validated for Flow, Func and IHC.

Target
Human CD274 / B7-H1 / PD-L1
Host
Mouse
Reactivity
Human, Non-Human Primates (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal
Clone
29E.2A3
Conjugations
Phycoerythrin. Also available Unconjugated.
Purification
Size exclusion chromatography
Modifications
Unmodified
Applications
  • IHC - Frozen
  • Flow Cytometry
  • Functional Assay
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Full length human CD274
Specificity
The mouse monoclonal antibody 29E.2A3 recognizes CD274 / PD-L1 (also known as B7-H1), a 40 kDa type I transmembrane protein expressed by dendritic cells, activated T cells, activated monocytes, and in various tissues, above all in heart and skeletal muscle, placenta and lung, and in many cancer cells, including T cell lymphomas, melanomas, and glioblastomas.
Usage
The reagent is designed for Flow Cytometry analysis of human blood cells using 10 ul reagent / 100 ul of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Presentation
PBS, 15mM sodium azide
Storage
Store at 2°C to 8°C. Do not freeze. Avoid prolonged exposure to light.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD274 / B7-H1 / PD-L1
Q9NZQ7 NM_014143 NP_054862.1

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Requested From: United States
Date Requested: 6/18/2019
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