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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD274 / B7‑H1 / PD‑L1 Antibody (clone 29E.2A3, PE) LS‑C742356
PD-L1 antibody LS-C742356 is a PE-conjugated mouse monoclonal antibody to PD-L1 (CD274 / B7-H1) from human and non-human primates. Validated for Flow, Func and IHC.
Catalog
Size
Price
LS-C742356-100
100 tst
$315
Description
PD-L1 antibody LS-C742356 is a PE-conjugated mouse monoclonal antibody to PD-L1 (CD274 / B7-H1) from human and non-human primates. Validated for Flow, Func and IHC.
Target
Human CD274 / B7-H1 / PD-L1
Host
Mouse
Reactivity
Human, Non-Human Primates (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal [29E.2A3]
Conjugations
Phycoerythrin. Also available Unconjugated.
Purification
Size exclusion chromatography
Modifications
Unmodified
Applications
  • IHC - Frozen
  • Flow Cytometry
  • Functional Assay
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Full length human CD274
Specificity
The mouse monoclonal antibody 29E.2A3 recognizes CD274 / PD-L1 (also known as B7-H1), a 40 kDa type I transmembrane protein expressed by dendritic cells, activated T cells, activated monocytes, and in various tissues, above all in heart and skeletal muscle, placenta and lung, and in many cancer cells, including T cell lymphomas, melanomas, and glioblastomas.
Presentation
PBS, 15 mM Sodium Azide
Storage
Store in the dark at 2°C to 8°C. Do not freeze. Protect from light.
Restrictions
For research use only.
About CD274 / B7-H1 / PD-L1
Q9NZQ7 NM_014143 NP_054862.1

Popular CD274 / B7-H1 / PD-L1 Products

Flow Cytometry Image
Species: Human
Applications: IHC, IHC - Paraffin, Flow Cytometry
Flow cytometry of B7-H1 / PD-L1 / CD274 antibody This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: Flow Cytometry, ELISA
Flow cytometry of B7-H1 / PD-L1 / CD274 antibody This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human
Applications: Flow Cytometry, ELISA
Flow cytometry of B7-H1 / PD-L1 / CD274 antibody
Species: Human
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Anti-B7-H1 / PD-L1 / CD274 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot

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Images

Flow Cytometry

Surface staining of CD274 on PHA-stimulated PBMC with anti-CD274 (29E.2A3) PE.
Surface staining of CD274 on PHA-stimulated PBMC with anti-CD274 (29E.2A3) PE.

Flow Cytometry

Surface staining of CD274 on PHA-stimulated PBMC with anti-CD274 (29E.2A3) PE.
Surface staining of CD274 on PHA-stimulated PBMC with anti-CD274 (29E.2A3) PE.

Flow Cytometry

Surface staining of CD274 on PHA-stimulated PBMC with anti-CD274 (29E.2A3) PE.
Surface staining of CD274 on PHA-stimulated PBMC with anti-CD274 (29E.2A3) PE.

Flow Cytometry

Surface staining of CD274 on PHA-stimulated PBMC with anti-CD274 (29E.2A3) PE.
Surface staining of CD274 on PHA-stimulated PBMC with anti-CD274 (29E.2A3) PE.

Requested From: United States
Date Requested: 12/16/2018
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