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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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APAF1 / APAF-1

apoptotic peptidase activating factor 1

Apaf1 has been described as the core of the apoptosome. Deficiency in murine Apaf1 leads to embryonic lethality with a phenotype affecting many aspects of developmental apoptosis. In the developing brain, Apaf1 is a death regulator of the neuronal founder cells. Combined intercrosses of mouse lines mutant for members of the mitochondrial death pathway are providing us with some clues about the relative regulation existing among neuronal cell populations. Apaf1-deficient embryos display an interesting phenotype in the inner ear and in limb development, which involves different caspase-dependent and -independent pathways. Moreover, APAF1 is mutated in human melanomas, and its depletion contributes to malignant transformation in a mouse model of cancer.

Gene Name: apoptotic peptidase activating factor 1
Family/Subfamily: Apoptosis , Not assigned-Other
Synonyms: APAF1, APAF-1, CED4, KIAA0413
Target Sequences: NM_013229 NP_037361.1 O14727

Filtered by: None
Target
APAF1 / APAF-1 (85)
Reactivity
Human (73)
Mouse (45)
Rat (26)
Bovine (1)
C. elegans (5)
Gerbil (2)
Guinea pig (1)
Horse (1)
Mammal (5)
Monkey (3)
Rabbit (2)
Application
IHC (54)
IHC-Fr (4)
IHC-P (18)
WB (75)
Flo (2)
ELISA (24)
ICC (10)
IF (4)
IP (17)
Peptide-ELISA (1)
Host
rabbit (73)
mouse (6)
rat (6)
Isotype
IgG (34)
IgG1 (2)
IgG1,k (2)
IgG2a (3)
IgG2a,k (3)
IgG2b (2)
Clonality
monoclonal mc (12)
polyclonal pc (73)
Clone
13F11 (1)
18H2 (1)
2E10 (1)
2E12 (1)
2H9A1 (1)
2H9G5 (1)
5C1 (1)
5E11 (1)
Format
AP Conjugated (2)
APC (2)
APC Conjugated (3)
Biotin Conjugated (7)
Cy3 Conjugated (2)
Cy7 Conjugated (2)
FITC Conjugated (7)
HRP Conjugated (5)
Low Endotoxin (1)
Magnetic beads Conjugated (1)
PE Conjugated (4)
Sepharose Conjugated (1)
Unconjugated (51)
Epitope
aa31-350 (10)
aa872-1135 (10)
N-Terminus (7)
aa1-97 (4)
aa12-28 (3)
C-Terminus (2)
aa1-464 (2)
501-550 aa, Internal (1)
His324 (1)
Internal (1)
aa1051-1248 (1)
aa264-282 (1)
aa289-338 (1)
aa451-468 (1)
Publications
No (85)
Showing 1-25 of 85 results

APAF1 / APAF-1 Antibody (aa1-464, clone 2E12, Low Endotoxin) IHC-plus™ LS-B12884
Rat Monoclonal [clone 2E12] (IgG2a,k) to Human APAF1 / APAF-1
Human
ELISA ICC IHC IHC-P IP WB
Unconjugated, Low Endotoxin
50µg 1mg/ml  $425.00
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) This image was taken for the unmodified form of this product. Other forms have not been tested.
Western blot analysis using anti-Apaf-1 (human), MAb (2E12) on several human cell lines and one monkey cell line (COS). This image was taken for the unmodified form of this product. Other forms have not been tested.

APAF1 / APAF-1 Antibody LS-C284
Rabbit Polyclonal (IgG) to Human APAF1 / APAF-1
Mouse, Rat, Human
ELISA IF IHC-P WB
Unconjugated
0.1mg 1mg/ml  $365.00
Immunohistochemistry of Apaf1 in human heart tissue with Apaf1 antibody at 1 ug/mL.
Immunofluorescence of Apaf1 in K562 cells with Apaf1 antibody at 10 ug/ml.

APAF1 / APAF-1 Antibody LS-C148201
Rabbit Polyclonal to Human APAF1 / APAF-1
Mouse, Rat, Gerbil, Human
IHC IHC-Fr IHC-P IP WB
Unconjugated
50µl  $335.00
Formalin-fixed, paraffin-embedded sections from a human brain tumor tissue microarray stained for Apaf 1 expression using APAF1 / APAF-1 at 1:2000. A and B. Tissue microarray over view. A1 and B1, higher magnification of cores from A and B, respectively. Differential expression of Apaf 1 is seen between patient samples. Hematoxylin-Eosin counterstain.
Formalin-fixed, paraffin-embedded tissue sections of human brain tumors stained for Apaf 1 expression using APAF1 / APAF-1 at 1:2000. A. Gemistocytoma (grade II). B. Anaplastic glioma. A high level of Apaf 1was observed in the gemistocytoma whereas a low level was seen in the more malignant anaplastic glioma. Hematoxylin-Eos in counterstain.

APAF1 / APAF-1 Antibody LS-C285
Rabbit Polyclonal (IgG) to Human APAF1 / APAF-1
Mouse, Rat, Human
ELISA IHC WB
Unconjugated
0.1mg 1mg/ml  $365.00
Immunohistochemistry of Apaf1 in human skin tissue with Apaf1 antibody at 20 µg/ml.
Western blot of Apaf1 in human heart tissue lysate with Apaf1 antibody at 1 ug/ml dilution in the absence (A) or presence (B) of blocking peptide.

APAF1 / APAF-1 Antibody (C-Terminus) IHC-plus™ LS-B3340
Rabbit Polyclonal to Human APAF1 / APAF-1
Mouse, Rat, Human
IHC IHC-P
Unconjugated
250µl 0.2mg/ml  $425.00
Anti-APAF1 / APAF-1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-APAF1 / APAF-1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

APAF1 / APAF-1 Antibody (aa289-338) IHC-plus™ LS-B7591
Rabbit Polyclonal (IgG) to Human APAF1 / APAF-1
Human
IHC IHC-P Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $425.00
Anti-APAF1 / APAF-1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Western blot analysis of lysates from COLO205 cells, using APAF-1-ALT Antibody. The lane on the right is blocked with the synthesized peptide.

APAF1 / APAF-1 Antibody (C-Terminus) LS-C351827
Rabbit Polyclonal to Human APAF1 / APAF-1
Human
ICC IF IHC IHC-P WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of APAF-1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of APAF-1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

APAF1 / APAF-1 Antibody LS-C659197
Rabbit Polyclonal (IgG) to Human APAF1 / APAF-1
Human
ELISA IF IHC IP WB
Unconjugated
100µg  $405.00

APAF1 / APAF-1 Antibody (clone 2H9A1) LS-C682621
Mouse Monoclonal [clone 2H9A1] (IgG2b) to Human APAF1 / APAF-1
Human
ELISA Flo ICC WB
Unconjugated
100µl  $365.00

APAF1 / APAF-1 Antibody (aa1-464) LS-C3751
Rat Monoclonal (IgG2a) to Human APAF1 / APAF-1
Human
ELISA ICC IHC IHC-Fr IP WB
Unconjugated
100µg  $620.00

APAF1 / APAF-1 Antibody (aa12-28) IHC-plus™ LS-B743
Rabbit Polyclonal to Human APAF1 / APAF-1
Mouse, Rat, Human
IHC IHC-P WB
Unconjugated
50µg 0.5mg/ml  $495.00
Anti-APAF1 / APAF-1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
Anti-APAF1 / APAF-1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.

APAF1 / APAF-1 Antibody (aa1-97) LS-C3750
Rat Monoclonal (IgG2a) to Mouse APAF1 / APAF-1
Mouse, Human
ELISA ICC IP WB
Unconjugated
100µg  $635.00

APAF1 / APAF-1 Antibody (His324) LS-C175921
Rabbit Polyclonal to Human APAF1 / APAF-1
Human
IF IHC IHC-P WB
Unconjugated
100µl  $345.00
IHC of APAF-1-ALT (H324) pAb in paraffin-embedded human lung carcinoma tissue.
Western blot of APAF-1-ALT (H324) pAb in extracts from COLO205 cells.

APAF1 / APAF-1 Antibody (aa1-97, clone 13F11) LS-C344794
Rat Monoclonal [clone 13F11] (IgG2a,k) to Mouse APAF1 / APAF-1
Mouse, Rat
ELISA ICC IP WB
Unconjugated
100µg  $375.00
Western blot analysis using anti-Apaf-1 (mouse/rat), MAb (13F11) on purified cells from mouse tissues. The ~130kDa band corresponds to endogenous Apaf-1, which is absent in cells from Apaf-1 KO mice.
Immunoprecipitation on mouse thymocytes using anti-Apaf-1 (mouse/rat), MAb (13F11).

APAF1 / APAF-1 Antibody (aa1-97, clone 18H2) LS-C344795
Rat Monoclonal [clone 18H2] (IgG2a,k) to Mouse APAF1 / APAF-1
Mouse, Human
ELISA ICC IP WB
Unconjugated
100µg  $375.00
Western blot analysis using anti-Apaf-1, mAb (18H2) on lysates from mouse cell lines.
Immunoprecipitation on mouse thymocytes using anti-Apaf-1, mAb (18H2).

APAF1 / APAF-1 Antibody LS-C405390
Rabbit Polyclonal (IgG) to Human APAF1 / APAF-1
Mouse, Rat, Human
ELISA IHC WB
Unconjugated
20µl 0.5mg/ml  $205.00; 60µl 0.5mg/ml  $235.00; 120µl 0.5mg/ml  $295.00; 200µl 0.5mg/ml  $395.00
Immunohistochemistry of paraffin-embedded Human thyroid cancer using APAF1 Polyclonal Antibody at dilution of 1:20.
Immunohistochemistry of paraffin-embedded Human cervical cancer using APAF1 Polyclonal Antibody at dilution of 1:20.

APAF1 / APAF-1 Antibody (aa451-468) LS-C148200
Rabbit Polyclonal to Human APAF1 / APAF-1
Human, Monkey
IHC IHC-Fr IHC-P IP WB
Unconjugated
50µl  $335.00
Formalin-fixed, paraffin-embedded tissue section of human pancreatic islets stained for Apaf 1 using APAF1 / APAF-1 Antibody at 1:2000. Hematoxylin-Eosin counterstain.

APAF1 / APAF-1 Antibody (501-550 aa, Internal) LS-C387145
Rabbit Polyclonal (IgG) to Human APAF1 / APAF-1
Mouse, Rat, Human
ELISA IHC WB
Unconjugated
100µl 1mg/ml  $265.00
Immunohistochemistry of Apaf-1 antibody
Immunohistochemistry of Apaf-1 antibody

APAF1 / APAF-1 Antibody (aa264-282) LS-C148199
Rabbit Polyclonal to Human APAF1 / APAF-1
Rabbit, Human
IHC IHC-Fr IHC-P IP WB
Unconjugated
50µl  $335.00
Formalin-fixed, paraffin-embedded tissue core of human glioma (grade II) stained for Apaf 1 expression using APAF1 / APAF-1 Antibody at 1:2000. A and A1. Low and high magnification, respectively Hematoxylin-Eosin counterstain.
Formalin-fixed, paraffin-embedded human brain tissue tissue sections stained for Apaf 1 expression using APAF1 / APAF-1 Antibody at 1:2000. A. Brain stem. B. Cortex. Hematoxylin-Eos in counterstain.

APAF1 / APAF-1 Antibody (clone 2H9G5) LS-C682644
Mouse Monoclonal [clone 2H9G5] (IgG2b) to Human APAF1 / APAF-1
Human
ELISA Flo WB
Unconjugated
100µl  $365.00

APAF1 / APAF-1 Antibody (aa1-97) LS-C3749
Rat Monoclonal (IgG2a) to Mouse APAF1 / APAF-1
Mouse, Rat
ICC IP WB
Unconjugated
100µg  $665.00

APAF1 / APAF-1 Antibody (aa31-350) LS-C314686
Rabbit Polyclonal to Human APAF1 / APAF-1
Human
IHC WB
Unconjugated
20µl 0.5mg/ml  $205.00; 100µl 0.5mg/ml  $255.00; 200µl 0.5mg/ml  $295.00; 1ml 0.5mg/ml  $495.00; 10ml 0.5mg/ml  $1,980.00
Immunohistochemistry - Paraffin Image
Immunohistochemistry - Paraffin Image

APAF1 / APAF-1 Antibody LS-C402084
Rabbit Polyclonal (IgG) to Human APAF1 / APAF-1
Mouse, Rat, Human
ELISA IHC WB
Unconjugated
20µl 0.7mg/ml  $205.00; 60µl 0.7mg/ml  $235.00; 120µl 0.7mg/ml  $295.00; 200µl 0.7mg/ml  $395.00
Immunohistochemistry of paraffin-embedded Human cervical cancer using APAF1 Polyclonal Antibody at dilution of 1:40.
Western blot analysis of HeLa cell, using APAF1 Polyclonal Antibody at dilution of 1:350.

APAF1 / APAF-1 Antibody (N-Terminus) LS-C178933
Rabbit Polyclonal to Human APAF1 / APAF-1
Human
ELISA IHC WB
Unconjugated
100µl 1mg/ml  $255.00
Immunohistochemistry Image
Western blot Image

APAF1 / APAF-1 Antibody LS-C69076
Rabbit Polyclonal (IgG) to Human APAF1 / APAF-1
Mouse, Rat, Human
IHC IP WB
Unconjugated
50µl  $445.00
Showing 1-25 of 85 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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