Products
Services
Research Areas
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Services
Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Reasearch Areas
Cell Cycle Pathways
Protein Family And Group
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, froforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order

IHCPlus™ APAF1 / APAF‑1 Antibody aa1‑464 Monoclonal Low Endotoxin ELISA,ICC,IHC,IP,WB LS‑B12884

Catalog Number Size Price
LS-B12884-50 50 µg (1 mg/ml) $425 
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) This image was taken for the unmodified form of this product. Other forms have not been tested.
Western blot analysis using anti-Apaf-1 (human), MAb (2E12) on several human cell lines and one monkey cell line (COS). This image was taken for the unmodified form of this product. Other forms have not been tested.
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) This image was taken for the unmodified form of this product. Other forms have not been tested.
Western blot analysis using anti-Apaf-1 (human), MAb (2E12) on several human cell lines and one monkey cell line (COS). This image was taken for the unmodified form of this product. Other forms have not been tested.
1 of 2
2 of 2

IHCPlus™ APAF1 / APAF‑1 Antibody aa1‑464 Monoclonal Low Endotoxin ELISA,ICC,IHC,IP,WB LS‑B12884

Note: This antibody replaces LS-C344792
Antibody:
Rat Monoclonal to Human APAF1 / APAF-1
Application:
IHC, IHC-P, ICC, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Low Endotoxin
Price
Catalog Number
$425 
LS-B12884-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rat Monoclonal to Human APAF1 / APAF-1
Application:
IHC, IHC-P, ICC, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Low Endotoxin

Specifications

Description
APAF-1 antibody LS-B12884 is an unconjugated rat monoclonal antibody to human APAF-1 (APAF1 ). Validated for ELISA, ICC, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human APAF1 / APAF-1
Host
Rat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
2E12
Conjugations
Unconjugated
Purification
Protein G purified from tissue culture supernatant
Modifications
Low Endotoxin
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • ICC
  • Western blot (1:1000 - 1:2000)
  • Immunoprecipitation (1:1000 - 1:2000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant human Apaf-1 (aa 1-464) containing the N-terminal CARD and CED-4 homologous domains.
Epitope
aa1-464
Specificity
Recognizes the CARD domain of human Apaf-1. Does not cross-react with mouse or rat Apaf-1.
Usage
Immunohistochemistry - EM on frozen sections, see G. Hausmann, et al.. (2000. Immunoprecipitation - 1:1000-1:2000. Western Blot - 1:1000-1:2000. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Presentation
PBS, 0.02% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About APAF1 / APAF-1
O14727 NM_013229 NP_037361.1

Validation

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) This image was taken for the unmodified form of this product. Other forms have not been tested.
IHC Validated
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) This image was taken for the unmodified form of this product. Other forms have not been tested.
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) This image was taken for the unmodified form of this product. Other forms have not been tested.

Publications (0)

Reviews (0)

Featured Products

Formalin-fixed, paraffin-embedded tissue sections of human brain tumors stained for Apaf 1 expression using APAF1 / APAF-1 at 1:2000. A. Gemistocytoma (grade II). B. Anaplastic glioma. A high level of Apaf 1was observed in the gemistocytoma whereas a low level was seen in the more malignant anaplastic glioma. Hematoxylin-Eos in counterstain.
Species: Human, Mouse, Rat, Gerbil
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Anti-APAF1 / APAF-1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin
Immunohistochemistry of Apaf1 in human skin tissue with Apaf1 antibody at 20 µg/ml.
Species: Human, Mouse, Rat
Applications: IHC, Western blot, ELISA
Immunohistochemistry of Apaf1 in human heart tissue with Apaf1 antibody at 1 ug/mL.
Species: Human, Mouse, Rat
Applications: IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Reactivity: Rat
Range: 0.156-10 ng/ml

Requested From: United States
Date Requested: 9/16/2019