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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
Protein Family And Group
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Catalog Number Size Price
LS-B12884-50 50 µg (1 mg/ml) $425 
APAF1 / APAF-1 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) This image was taken for the unmodified form of this product. Other forms have not been tested.
APAF1 / APAF-1 Antibody - Western blot analysis using anti-Apaf-1 (human), MAb (2E12) on several human cell lines and one monkey cell line (COS). This image was taken for the unmodified form of this product. Other forms have not been tested.
APAF1 / APAF-1 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) This image was taken for the unmodified form of this product. Other forms have not been tested.
APAF1 / APAF-1 Antibody - Western blot analysis using anti-Apaf-1 (human), MAb (2E12) on several human cell lines and one monkey cell line (COS). This image was taken for the unmodified form of this product. Other forms have not been tested.
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IHCPlus™ Monoclonal Rat anti‑Human APAF1 / APAF‑1 Antibody (clone 2E12, Low Endotoxin, aa1‑464, IHC, WB) LS‑B12884

IHCPlus™ Monoclonal Rat anti‑Human APAF1 / APAF‑1 Antibody (clone 2E12, Low Endotoxin, aa1‑464, IHC, WB) LS‑B12884

Note: This antibody replaces LS-C344792
Antibody:
Rat Monoclonal to Human APAF1 / APAF-1
Application:
IHC, IHC-P, ICC, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Low Endotoxin
Price
Catalog Number
$425 
LS-B12884-50
50 µg
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rat Monoclonal to Human APAF1 / APAF-1
Application:
IHC, IHC-P, ICC, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Low Endotoxin

Specifications

Description
APAF-1 antibody LS-B12884 is an unconjugated rat monoclonal antibody to human APAF-1 (APAF1) (aa1-464). Validated for ELISA, ICC, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human APAF1 / APAF-1
Synonyms
APAF1 | APAF-1 | CED4 | KIAA0413
Host
Rat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
2E12
Conjugations
Unconjugated
Purification
Protein G purified from tissue culture supernatant
Modifications
Low Endotoxin
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • ICC
  • Western blot (1:1000 - 1:2000)
  • Immunoprecipitation (1:1000 - 1:2000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant human Apaf-1 (aa 1-464) containing the N-Terminus CARD and CED-4 homologous domains.
Epitope
aa1-464
Specificity
Recognizes the CARD domain of human Apaf-1. Does not cross-react with mouse or rat Apaf-1.
Usage
Immunohistochemistry - EM on frozen sections, see G. Hausmann, et al.. (2000. Immunoprecipitation - 1:1000-1:2000. Western Blot - 1:1000-1:2000. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Presentation
PBS, 0.02% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About APAF1 / APAF-1
O14727 NM_013229 NP_037361.1

Validation

APAF1 / APAF-1 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) This image was taken for the unmodified form of this product. Other forms have not been tested.
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) This image was taken for the unmodified form of this product. Other forms have not been tested.
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) This image was taken for the unmodified form of this product. Other forms have not been tested.

LSBio Ratings

IHC-plus™ APAF1 / APAF-1 Antibody (aa1-464, clone 2E12, Low Endotoxin) for IHC, ICC, WB/Western, IP, ELISA LS-B12884 has an LSBio Rating of
Laboratory Validation Score (4)

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Requested From: United States
Date Requested: 7/13/2020