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APAF1 / APAF‑1 Antibody LS‑C284

Catalog Number Size Price
LS-C284-0.1 0.1 mg $365 
Immunohistochemistry of Apaf1 in human heart tissue with Apaf1 antibody at 1 ug/mL.
Immunofluorescence of Apaf1 in K562 cells with Apaf1 antibody at 10 ug/ml.
Western blot of Apaf1 in K562 cell lysate with Apaf1 antibody at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.
Immunohistochemistry of Apaf1 in human heart tissue with Apaf1 antibody at 1 ug/mL.
Immunofluorescence of Apaf1 in K562 cells with Apaf1 antibody at 10 ug/ml.
Western blot of Apaf1 in K562 cell lysate with Apaf1 antibody at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.
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APAF1 / APAF‑1 Antibody LS‑C284

Antibody:
Rabbit Polyclonal to Human APAF1 / APAF-1
Application:
IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$365 
LS-C284-0.1
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human APAF1 / APAF-1
Application:
IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
APAF-1 antibody LS-C284 is an unconjugated rabbit polyclonal antibody to APAF-1 (APAF1 ) from human, mouse and rat. Validated for ELISA, IF, IHC and WB.
Target
Human APAF1 / APAF-1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Applications
  • IHC - Paraffin
  • Immunofluorescence
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Failed Applications
  • IHC - Paraffin
Immunogen
a peptide corresponding to amino acids near the N-terminus of human Apaf1. The sequences of the immunogenic peptide are identical between human and mouse. The immunogen is located within the first 50 amino acids of Apaf1.
Blocking Peptide
LS-E29868 - Liquid - 0.05 mg - $145.00
Immunizing peptide used to generate LS-C284. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Usage
Apaf1 antibody can be used for detection of Apaf1 by Western blot at 1 ug/mL. A 115 - 130 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 1 ug/mL. For immunofluorescence start at 10 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 132 kDa Observed: 130 kDa
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About APAF1 / APAF-1
O14727 NM_013229 NP_037361.1

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Requested From: United States
Date Requested: 8/18/2019
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