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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C285-0.1 0.1 mg $365 
APAF1 / APAF-1 Antibody - Immunohistochemistry of Apaf1 in human skin tissue with Apaf1 antibody at 20 µg/ml.
APAF1 / APAF-1 Antibody - Western blot of Apaf1 in human heart tissue lysate with Apaf1 antibody at 1 ug/ml dilution in the absence (A) or presence (B) of blocking peptide.
APAF1 / APAF-1 Antibody - Immunohistochemistry of Apaf1 in human skin tissue with Apaf1 antibody at 20 µg/ml.
APAF1 / APAF-1 Antibody - Western blot of Apaf1 in human heart tissue lysate with Apaf1 antibody at 1 ug/ml dilution in the absence (A) or presence (B) of blocking peptide.
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APAF1 / APAF‑1 Antibody LS‑C285

APAF1 / APAF‑1 Antibody LS‑C285

Antibody:
Rabbit Polyclonal to Human APAF1 / APAF-1
Application:
IHC, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$365 
LS-C285-0.1
0.1 mg
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human APAF1 / APAF-1
Application:
IHC, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
APAF-1 antibody LS-C285 is an unconjugated rabbit polyclonal antibody to APAF-1 (APAF1 ) from human, mouse and rat. Validated for ELISA, IHC and WB.
Target
Human APAF1 / APAF-1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Applications
  • IHC
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Failed Applications
  • IHC - Paraffin
Immunogen
a peptide corresponding to amino acids near the C-terminus of human Apaf1. The sequence of the immunogenic peptide differs from that of murine Apaf1 by one amino acid. The immunogen is located within the last 50 amino acids of Apaf1.
Blocking Peptide
LS-E29869 - Liquid - 0.05 mg - $145.00
Immunizing peptide used to generate LS-C285. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Usage
Apaf1 antibody can be used for detection of Apaf1 by Western blot at 1 ug/mL. Human heart tissue lysate can be used as positive control and a 130 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 20 ug/mL. Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Western Blot: 130 kDa
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About APAF1 / APAF-1
O14727 NM_013229 NP_037361.1

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Requested From: United States
Date Requested: 11/12/2019