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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Results for "RELA OR RELB OR REL OR NFKB1 OR NFKB2 OR NFKB" Filtered by: None
Target
Actin (1)
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Angiostatin K1-3 (1)
ANXA5 / Annexin V (1)
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Human (999+)
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rabbit (999+)
mouse (173)
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Isotype
IgG (661)
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monoclonal mc (193)
polyclonal pc (999+)
Clone
10000000 (1)
112A1021 (2)
1298CT792.105.117.133 (1)
12K142 (1)
1684CT450.20.25 (1)
17.3 (2)
184C278 (2)
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318CT41.3.2 (7)
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64C1385.1 (2)
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Epitope
C-Terminus (84)
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pSer536 (23)
Internal (19)
aa19-306 (18)
aa81-270 (17)
aa290-377 (16)
aa1-296 (11)
pSer529 (11)
aa131-369 (10)
aa145-327 (10)
aa176-436 (10)
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aa944-1148 (10)
aa286-373 (9)
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aa1-30 (8)
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aa251-300 (8)
aa267-296 (8)
aa32-60 (8)
aa35-225 (8)
aa36-64 (8)
aa36-85 (8)
aa387-415 (8)
aa40-365 (8)
aa42-367 (8)
aa463-492 (8)
aa48-76 (8)
aa586-619 (8)
aa78-265 (8)
aa805-892 (8)
aa81-266 (8)
aa848-874 (8)
aa88-114 (8)
aa911-937 (8)
aa516-542 (7)
pSer311 (7)
pSer927 (7)
pSer932 (7)
pThr254 (7)
470-550 aa (6)
pSer893 (6)
pThr505 (6)
810-890 aa (5)
pSer907 (5)
pThr435 (5)
250-330 aa (4)
280-360 aa (4)
440-520 aa (4)
870-950 aa (4)
aa110-159 (4)
aa161-210 (4)
aa179-228 (4)
aa21-70 (4)
aa211-260 (4)
aa269-318 (4)
aa29-44 (4)
aa31-80 (4)
aa326-616 (4)
aa358-407 (4)
aa380-429 (4)
aa395-444 (4)
aa451-500 (4)
aa48-97 (4)
aa501-550 (4)
aa503-552 (4)
aa61-110 (4)
aa65-114 (4)
aa711-758 (4)
aa756-805 (4)
pSer503 (4)
pSer552 (4)
pSer865 (4)
pSer869 (4)
aa50-100 (3)
aa501-551 (3)
aa526-539 (3)
aa96-113 (3)
pSer281 (3)
190-270 aa (2)
210-290 aa (2)
220-300 aa (2)
370-450 aa (2)
410-490 aa (2)
510-590 aa (2)
830-910 aa (2)
Publications
No (999+)
Yes (14)
Showing 1-25 of 1523 results for RELA OR RELB OR REL OR NFKB1 OR NFKB2 OR NFKB

RELA / NFKB p65 Antibody (phospho-Ser529) IHC-plus™ LS-B652
Publications (4)
Publications (4)
Rabbit Polyclonal to Human RELA / NFKB p65
Rabbit, Pig, Horse, Human, Monkey
ELISA IHC IHC-P IP WB
Unconjugated
50µl 75mg/ml  $425.00
Anti-RELA antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-RELA antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

RELA / NFKB p65 Antibody (C-Terminus) IHC-plus™ LS-B653
Publications (9)
Publications (9)
Rabbit Polyclonal to Human RELA / NFKB p65
Mouse, Rat, Human
ELISA GS IF IHC IHC-P IP WB
Unconjugated
50µl 80mg/ml  $425.00
Anti-RELA antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-RELA antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

RELA / NFKB p65 Antibody (Acetyl-Lys122) LS-C387648
Rabbit Polyclonal (IgG) to Human RELA / NFKB p65
Mouse, Rat, Human
ELISA WB
Unconjugated
100µl 1mg/ml  $265.00
Western blot of Acetyl-NFkappaB-p65 (K122) antibody
Western blot of Acetyl-NFkappaB-p65 (K122) antibody

RELA / NFKB p65 Antibody LS-C413576
Mouse Monoclonal to Human RELA / NFKB p65
Mouse, Rat, Human
ICC IF IHC IHC-P IP WB
Unconjugated
100µl 1mg/ml  $275.00
Immunohistochemical analysis of NF-kappaB p65 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of NF-kappaB p65 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

RELA / NFKB p65 Antibody (C-Terminus, clone 27F9.G4) LS-C153771
Publications (1)
Publications (1)
Mouse Monoclonal [clone 27F9.G4] (IgG) to Human RELA / NFKB p65
Human
ELISA ICC IF IHC WB
Unconjugated
100µg 1mg/ml  $425.00
Monoclonal anti NFKB p65 (Rel A) antibody was used to detect p65 by immunofluorescence at a dilution of 1:5000. HeLa cells were grown to sub-confluent on 18 mm2 glass coverslips #1.5. Cells were either unstimulated (A), or stimulated (B) with 50 ng/ml of TNF alpha for 30 min prior fixation. Cells were then fixed in methanol and blocked with 10% normal goat serum (NGS), in PBS, and Triton X 0.2% (Tx) and incubated for 1 hr at RT with primary ab, counterstained with DAPI and washed in PBS/NGS/Tx. Cells were incubated for 1 hr at RT with Atto 425 conjugated anti mouse secondary antibody for STED CW imaging. Data was collected on a STED-CW TCS-SP5 Confocal system equipped with a DFC 350FX camera allowing sequential acquisition in wide field, confocal and STED CW imaging on the same system.
anti NFKB p65 (Rel A) monoclonal antibody Anti-NFKB p65 (Rel A) (MOUSE) Monoclonal Antibody - 200-301-065 was used to detect ~65 kD band (red arrow) in HeLa whole cell lysate. Lysate was run on 4-20% gradient gel transferred under standard conditions and blocked in 1% BSA-TTBS 30 min RT. Blot was probed with monoclonal anti p65 1:1000 in 1% BSA-TBS-T o/n 4? and detected with HRP conjugated Rb-anti Mouse antibody 1:40000 in MB-070 30 min RT.

RELA / NFKB p65 Antibody IHC-plus™ LS-B53
Rabbit Polyclonal to Human RELA / NFKB p65
Human
ELISA IHC IHC-P WB
Unconjugated
50µg 1mg/ml  $485.00
Anti-RELA antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-p65 (RelA) pS536 Antibody - Western Blot. Western blot of affinity purified anti-p65 (RelA) pS536 antibody shows detection of p65 phosphorylated at S536. The control blot (left lane) contains 100 ng of purified p65-GST fusion protein. The band is seen (right lane) when this protein is phosphorylated at S536 by IKKbeta. Personal Communication. J. Brady, NCI, Bethesda, MD.

RELA / NFKB p65 Antibody (clone 8G3) IHC-plus™ LS-B13554
Mouse Monoclonal [clone 8G3] (IgG1,l) to Human RELA / NFKB p65
Human
ELISA IF IHC IHC-P WB
Unconjugated
50µg  $485.00
Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence of monoclonal antibody to RELA on HeLa cell. [antibody concentration 35 ug/ml]

RELA / NFKB p65 Antibody (aa501-551) LS-C290611
Goat Polyclonal to Human RELA / NFKB p65
Mouse, Human
IP PLA WB
Unconjugated
100µl 1mg/ml  $395.00
Detection of Human RelA by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. Antibodies: Affinity purified goat anti-RelA antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of Mouse RelA by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, Renca, and TCMK-1 cells. Antibodies: Affinity purified goat anti-RelA antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

RELA / NFKB p65 Antibody (aa143-158) LS-C312835
Rabbit Polyclonal to Human RELA / NFKB p65
Mouse, Rat, Human
ICC IHC IHC-P WB
Unconjugated
100µg 200µl 0.5mg/ml  $355.00
WB of RELA / NFKB p65 antibody. All lanes: Anti-RELA at 0.5ug/ml. Lane 1: Rat Testis Tissue Lysate at 40ug. Lane 2: HELA Whole Cell Lysate at 40ug. Lane 3: A431 Whole Cell Lysate at 40ug. Lane 4: JURKAT Whole Cell Lysate at 40ug. Predicted bind size: 60KD. Observed bind size: 64KD.

RELA / NFKB p65 Antibody (N-Terminus) LS-C18905
Publications (3)
Publications (3)
Rabbit Polyclonal to Human RELA / NFKB p65
Human
ELISA IF IHC IHC-P WB
Unconjugated
100µg 1mg/ml  $425.00
Anti-p65 NLS Antibody - Immunohistochemistry. Rabbit anti-p65 NLS was used at a 1:200 dilution to detect p65 in (A) control DU145 cells and (B) TNF stimulated DU145 cells. Although DU145 show relatively high basal levels of nuclear p65 staining, significant enhancement of nuclear staining is seen in panel B as evidence of translocation and availability of the NLS to be bound by the antibody. Cultured cells shown above were formalin-fixed. tissue staining (not shown) were formalin-fixed, paraffin embedded followed by citrate retrieval. Blocking and hybridization included 5% NGS.
Anti-p65 NLS Antibody - Immunofluorescence Microscopy. Rabbit anti-p65 NLS was used at a 1:200 dilution to detect p65 in TNF stimulated DU145 cells. Image shown is at a 1:400 magnification. Tissue was fixed and prepared as above.

RELA / NFKB p65 Antibody (Acetyl-Lys310) LS-C354091
Rabbit Polyclonal to Human RELA / NFKB p65
Mouse, Bovine, Rat, Pig, Human
ChrIP ICC IF IHC IHC-P WB
Unconjugated
100µl 1mg/ml  $265.00
Immunohistochemical analysis of NF-kappaB p65 (AcK310) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of NF-kappaB p65 (AcK310) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

RELA / NFKB p65 Antibody (phospho-Ser276) LS-C154220
Publications (1)
Publications (1)
Rabbit Polyclonal (IgG) to Human RELA / NFKB p65
Human
ELISA WB
Unconjugated
100µg 1mg/ml  $425.00
anti-NFkB antibody. Affinity purified Anti-NFKB p65 (Rel A) pS276 Anti-NFKB p65 (Rel A) pS276 (RABBIT) Antibody - 600-401-264 lot 24040 was probed against Normal (Lane 1) and TNFalpha (Lane 2) Stimulated HeLa whole cell lysates. A band was observed between 55 and 72 kD corresponding to the expected MW of NFkB p65. The observed higher MW bands have not been characterized.

RELA / NFKB p65 Antibody (Internal) LS-C352790
Rabbit Polyclonal to Human RELA / NFKB p65
Mouse, Bovine, Rat, Pig, Human, Monkey
IHC IHC-P IP WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of NF-kappaB p65 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Western blot analysis of NF-kappaB p65 expression in HepG2 (A); MCF7 (B); Raw264.7 (C); rat liver (D) whole cell lysates.

RELA / NFKB p65 Antibody (C-Terminus) LS-C41295
Rabbit Polyclonal (IgG) to Mouse RELA / NFKB p65
Mouse, Rat, Human
ICC WB
Unconjugated
50µl  $225.00
Hela cells stained with purified rabbit polyclonal antibody against NF-kappaB p65 (poly6226), followed by Rhodamine Red-X conjugated goat anti-rabbit IgG and DAPI
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-NF-kappaB p65 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.

RELA / NFKB p65 Antibody (mono-methylation site of K314/K315) LS-C380562
Rabbit Polyclonal (IgG) to Human RELA / NFKB p65
Mouse, Rat, Human
ELISA WB
Unconjugated
100µl 1mg/ml  $265.00
Western blot of Mono-Methyl-NFkappaB-p65 (K314/K315) antibody

RELA / NFKB p65 Antibody LS-C497795
Rabbit Monoclonal to Human RELA / NFKB p65
Mouse, Human
Flo IF IHC IP WB
Unconjugated
50µl  $295.00; 100µl  $385.00; 200µl  $560.00
Immunohistochemistry of paraffin-embedded mouse lung using RELA antibodyat dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human mammary cancer using RELA antibodyat dilution of 1:100 (40x lens).

RELA / NFKB p65 Antibody (aa297-308) LS-C9296
Rabbit Polyclonal (IgG) to Human RELA / NFKB p65
Mouse, Dog, Bovine, Rat, Hamster, Horse, Bat, Human, Monkey
ELISA IF IHC WB
Unconjugated
100µg  $755.00

RELA / NFKB p65 Antibody (250-330 aa) LS-C380594
Rabbit Polyclonal (IgG) to Human RELA / NFKB p65
Mouse, Human
ELISA IF IHC WB
Unconjugated
100µl 1mg/ml  $265.00
Western blot of Acetyl-NFkappaB-p65 (K310) antibody
Western blot of Acetyl-NFkappaB-p65 (K310) antibody

RELA / NFKB p65 Antibody (Biotin) LS-C181888
Rabbit Polyclonal to Human RELA / NFKB p65
Mouse, Human, Primate
ICC IF IHC IHC-P WB
Biotin Conjugated
100µl 1mg/ml  $365.00

RELA / NFKB p65 Antibody (aa275-324) IHC-plus™ LS-B12541
Rabbit Polyclonal (IgG) to Human RELA / NFKB p65
Mouse, Human
IF IHC IHC-P Peptide-ELISA WB
Unconjugated
100µl 1mg/ml  $505.00
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

RELA / NFKB p65 Antibody (phospho-Ser276) LS-C335799
Rabbit Polyclonal (IgG) to Human RELA / NFKB p65
IF IHC WB
Unconjugated
50µl  $265.00; 100µl  $375.00; 200µl  $495.00
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue.
Immunofluorescence staining of methanol-fixed HeLa cells.

RELA / NFKB p65 Antibody (phospho-Ser536) LS-C335800
Rabbit Polyclonal (IgG) to Human RELA / NFKB p65
IF IHC WB
Unconjugated
50µl  $265.00; 100µl 1.08mg/ml  $375.00; 200µl  $495.00
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue.
Immunofluorescence staining of methanol-fixed Hela cells.

RELA / NFKB p65 Antibody IHC-plus™ LS-B6523
Rabbit Polyclonal to Human RELA / NFKB p65
Human
IHC IHC-P WB
Unconjugated
50µg 0.5mg/ml  $425.00
Anti-RELA antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Western blot of NF-kB p65-NLS in Jurkat cell lysate using antibody at 1 ug/ml.

RELA / NFKB p65 Antibody (phospho-Ser536) LS-C352793
Rabbit Polyclonal to Human RELA / NFKB p65
Mouse, Rat, Pig, Human, Monkey
IHC IHC-P IP WB
Unconjugated
100µl 1mg/ml  $265.00
Immunohistochemical analysis of NF-kappaB p65 (pS536) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Western blot analysis of NF-kappaB p65 (pS536) expression in HeLa TNFa-treated (A); NIH3T3 LPS-treated (B); rat brain (C) whole cell lysates.

RELA / NFKB p65 Antibody (phospho-Ser529) LS-C354093
Rabbit Polyclonal to Human RELA / NFKB p65
Mouse, Rat, Human, Monkey
IHC IHC-P IP WB
Unconjugated
100µl 1mg/ml  $265.00
Immunohistochemical analysis of NF-kappaB p65 (pS529) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Western blot analysis of NF-kappaB p65 (pS529) expression in MCF7 UV-treated (A); Raw264.7 TNFa-treated (B); rat liver (C); rat kidney (D) whole cell lysates.
Showing 1-25 of 1523 results for RELA OR RELB OR REL OR NFKB1 OR NFKB2 OR NFKB

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