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RELA / NFKB p65

v-rel avian reticuloendotheliosis viral oncogene homolog A

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1.

Gene Name: v-rel avian reticuloendotheliosis viral oncogene homolog A
Synonyms: RELA, NF-kappa-B p65delta3, NF-kappaB, NFKB3, p65, Transcription factor p65, NF kappaB, NFKB
Target Sequences: NM_021975 NP_068810.3 Q04206

Publications (19)

1
Curcumin blocks cytokine-mediated NF-kappa B activation and proinflammatory gene expression by inhibiting inhibitory factor I-kappa B kinase activity. Jobin C, Bradham CA, Russo MP, Juma B, Narula AS, Brenner DA, Sartor RB. Journal of immunology (Baltimore, Md. : 1950). 1999 163:3474-83. [PubMed:10477620] Related Antibodies: LS-B653.
2
Piceatannol inhibits TNF-induced NF-kappaB activation and NF-kappaB-mediated gene expression through suppression of IkappaBalpha kinase and p65 phosphorylation. Ashikawa K, Majumdar S, Banerjee S, Bharti AC, Shishodia S, Aggarwal BB. Journal of immunology (Baltimore, Md. : 1950). 2002 169:6490-7. [PubMed:12444159] Related Antibodies: LS-B652, LS-C154220.
3
Upregulation of HMG1 leads to melanoma inhibitory activity expression in malignant melanoma cells and contributes to their malignancy phenotype. Poser I, Golob M, Buettner R, Bosserhoff AK. Molecular and cellular biology. 2003 23:2991-8. [PubMed:12665595] [PMC:PMC152547] Related Antibodies: LS-B653.
4
Diabetes-induced activation of canonical and noncanonical nuclear factor-kappaB pathways in renal cortex. Starkey JM, Haidacher SJ, LeJeune WS, Zhang X, Tieu BC, Choudhary S, Brasier AR, Denner LA, Tilton RG. Diabetes. 2006 55:1252-9. [PubMed:16644679] Related Antibodies: LS-C18905.
5
NF-kappaB1 (p50) homodimers differentially regulate pro- and anti-inflammatory cytokines in macrophages. Cao S, Zhang X, Edwards JP, Mosser DM. The Journal of biological chemistry. 2006 281:26041-50. [PubMed:16835236] [PMC:PMC2642587] Related Antibodies: LS-B653.
6
Antiinflammatory adaptation to hypoxia through adenosine-mediated cullin-1 deneddylation. Khoury J, Ibla JC, Neish AS, Colgan SP. The Journal of clinical investigation. 2007 117:703-11. [PubMed:17318263] [PMC:PMC1797604] Related Antibodies: LS-C18905.
7
Glutathione depletion down-regulates tumor necrosis factor alpha-induced NF-kappaB activity via IkappaB kinase-dependent and -independent mechanisms. Lou H, Kaplowitz N. The Journal of biological chemistry. 2007 282:29470-81. [PubMed:17690092] Related Antibodies: LS-B653.
8
The phosphatidylinositol 3-kinase/Akt pathway negatively regulates Nod2-mediated NF-kappaB pathway. Zhao L, Lee JY, Hwang DH. Biochemical pharmacology. 2008 75:1515-25. [PubMed:18243161] Related Antibodies: LS-B652.
9
CXCL5 promotes prostate cancer progression. Begley LA, Kasina S, Mehra R, Adsule S, Admon AJ, Lonigro RJ, Chinnaiyan AM, Macoska JA. Neoplasia (New York, N.Y.). 2008 10:244-54. [PubMed:18320069] [PMC:PMC2262133] Related Antibodies: LS-B653.
10
Protein kinase A-induced phosphorylation of the p65 subunit of nuclear factor-kappaB promotes Schwann cell differentiation into a myelinating phenotype. Yoon C, Korade Z, Carter BD. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2008 28:3738-46. [PubMed:18385332] Related Antibodies: LS-B653.
11
Adenosine signaling mediates SUMO-1 modification of IkappaBalpha during hypoxia and reoxygenation. Liu Q, Li J, Khoury J, Colgan SP, Ibla JC. The Journal of biological chemistry. 2009 284:13686-95. [PubMed:19297320] [PMC:PMC2679470] Related Antibodies: LS-C18905.
12
Role of NF-kappaB in flow-induced vascular remodeling. Castier Y, Ramkhelawon B, Riou S, Tedgui A, Lehoux S. Antioxidants & redox signaling. 2009 11:1641-9. [PubMed:19320561] Related Antibodies: LS-B652.
13
Her2 activates NF-kappaB and induces invasion through the canonical pathway involving IKKalpha. Merkhofer EC, Cogswell P, Baldwin AS. Oncogene. 2010 29:1238-48. [PubMed:19946332] [PMC:PMC2829103] Related Antibodies: LS-C153771.
14
Axonal neuregulin 1 type III activates NF-kappaB in Schwann cells during myelin formation. Limpert AS, Carter BD. The Journal of biological chemistry. 2010 285:16614-22. [PubMed:20360002] [PMC:PMC2878070] Related Antibodies: LS-B653.
15
Nuclear factor-B contributes to neuron-dependent induction of glutamate transporter-1 expression in astrocytes. Ghosh M, Yang Y, Rothstein JD, Robinson MB. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2011 31:9159-69. [PubMed:21697367] [PMC:PMC3138498] Related Antibodies: LS-B653.
16
Anti-inflammatory and anti-catabolic effects of TENDOACTIVE on human tenocytes in vitro. Shakibaei M, Buhrmann C, Mobasheri A. Histology and histopathology. 2011 26:1173-85. [PubMed:21751149] Related Antibodies: LS-B652.
17
Molecular targeting of renal inflammation using drug delivery technology to inhibit NF-?B improves renal recovery in chronic kidney disease. Alejandro R Chade, Maxx L Williams, Jason E Engel, Erika Williams, Gene L Bidwell rd. American journal of physiology. Renal physiology. 2020 July;319:F139-F148. [Full Text Article] [PubMed:32538151] [PMC:PMC7468833] Related Antibodies: LS-C290611.
18
Aggravated endothelial endocrine dysfunction and intimal thickening of renal artery in high-fat diet-induced obese pigs following renal denervation. Enyong Su, Linwei Zhao, Xiaohang Yang, Binbin Zhu, Yahui Liu, Wen Zhao, Xianpei Wang, Datun Qi, Lijie Zhu, Chuanyu Gao. BMC cardiovascular disorders. 2020 Apr;20:176. [Full Text Article] [PubMed:32295540] [PMC:PMC7161153] Related Antibodies: LS-B652.
19
Dexamethasone attenuates NF-kappa B DNA binding activity without inducing I kappa B levels in rat brain in vivo. Unlap MT, Jope RS. Brain research. Molecular brain research. 1997 45:83-9. [PubMed:9105673] Related Antibodies: LS-B653.
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RELA / NFKB p65 Antibody - Anti-RELA antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
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RELA / NFKB p65 Rabbit anti-Human Polyclonal (pSer529) Antibody
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50 µl/$485
RELA / NFKB p65 Antibody - Anti-RELA antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
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RELA / NFKB p65 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Mouse, Rat, Human
ChrIP, ELISA, GS, IHC, IHC-P, WB
Unconjugated
50 µl/$485
RELA / NFKB p65 Antibody - Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)
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RELA / NFKB p65 Mouse anti-Human Monoclonal (8G3) Antibody
Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
50 µg/$440
RELA / NFKB p65 Antibody - Detection of Human RelA by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. Antibodies: Affinity purified goat anti-RelA antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
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RELA / NFKB p65 Goat anti-Human Polyclonal (aa501-551) Antibody
Mouse, Human
IP, WB
Unconjugated
100 µl/$483
RELA / NFKB p65 Antibody - Anti-RELA antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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RELA / NFKB p65 Rabbit anti-Human Polyclonal (pSer536) Antibody
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50 µg/$485
RELA / NFKB p65 Antibody - Immunohistochemical analysis of NF-kappaB p65 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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RELA / NFKB p65 Mouse anti-Human Monoclonal Antibody
Mouse, Rat, Human
ICC, IF, IHC, IHC-P, IP, WB
Unconjugated
100 µl/$311
RELA / NFKB p65 Antibody - Western blot of Acetyl-NFkappaB-p65 (K122) antibody
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RELA / NFKB p65 Rabbit anti-Human Polyclonal (Acetyl-Lys122) Antibody
Mouse, Rat, Human
ELISA, WB
Unconjugated
50 µl/$279; 100 µl/$309; 200 µl/$379
RELA / NFKB p65 Antibody - A: Marker, B: HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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RELA / NFKB p65 Rabbit anti-Human Polyclonal (aa21-70) Antibody
Mouse, Guinea pig, Bovine, Rat, Horse, Human, Monkey
WB
Unconjugated
100 µl/$387
RELA / NFKB p65 Antibody - Immunohistochemical analysis of NF-kappaB p65 (AcK310) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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RELA / NFKB p65 Rabbit anti-Human Polyclonal (Acetyl-Lys310) Antibody
Mouse, Bovine, Rat, Pig, Human
ChrIP, ICC, IF, IHC, IHC-P, WB
Unconjugated
100 µl/$305
RELA / NFKB p65 Antibody - Monoclonal anti NFKB p65 (Rel A) antibody was used to detect p65 by immunofluorescence at a dilution of 1:5000. HeLa cells were grown to sub-confluent on 18 mm2 glass coverslips #1.5. Cells were either unstimulated (A), or stimulated (B) with 50 ng/ml of TNF alpha for 30 min prior fixation. Cells were then fixed in methanol and blocked with 10% normal goat serum (NGS), in PBS, and Triton X 0.2% (Tx) and incubated for 1 hr at RT with primary ab, counterstained with DAPI and washed in PBS/NGS/Tx. Cells were incubated for 1 hr at RT with Atto 425 conjugated anti mouse secondary antibody for STED CW imaging. Data was collected on a STED-CW TCS-SP5 Confocal system equipped with a DFC 350FX camera allowing sequential acquisition in wide field, confocal and STED CW imaging on the same system.
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RELA / NFKB p65 Mouse anti-Human Monoclonal (C-Terminus) (27F9.G4) Antibody
Human
ELISA, ICC, IF, IHC, WB
Unconjugated
100 µg/$498
RELA / NFKB p65 Antibody - Anti-NF-kB p65 antibody, IHC(P): Human Mammary Cancer Tissue
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RELA / NFKB p65 Rabbit anti-Human Polyclonal (aa143-158) Antibody
Mouse, Rat, Human
ICC, IHC, IHC-P, WB
Unconjugated
100 µg/$421
RELA / NFKB p65 ELISA Kit
Select
Sandwich
96-Well Strip Plate
Human
0.156 - 10 ng/ml
Colorimetric - 450nm (TMB)
Cell Lysates, Tissue Homogenates
1 Plate/$752
RELA / NFKB p65 Protein - Recombinant V-Rel Reticuloendotheliosis Viral Oncogene Homolog A By SDS-PAGE
Select
E. coli
His, N-Terminal
36.8 kDa
10 µg/$252; 50 µg/$329; 100 µg/$489; 200 µg/$612; 1 mg/$1,482; 500 µg/$1,129; 5 mg/$3,073
RELA / NFKB p65 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
60 kDa
20 µg/$200
RELA / NFKB p65 Antibody - Anti-p65 NLS Antibody - Immunohistochemistry. Rabbit anti-p65 NLS was used at a 1:200 dilution to detect p65 in (A) control DU145 cells and (B) TNF stimulated DU145 cells. Although DU145 show relatively high basal levels of nuclear p65 staining, significant enhancement of nuclear staining is seen in panel B as evidence of translocation and availability of the NLS to be bound by the antibody. Cultured cells shown above were formalin-fixed. tissue staining (not shown) were formalin-fixed, paraffin embedded followed by citrate retrieval. Blocking and hybridization included 5% NGS.
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RELA / NFKB p65 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, GS, IF, IHC, IHC-P, WB
Unconjugated
100 µg/$498
RELA / NFKB p65 Antibody - anti-NFkB antibody. Affinity purified Anti-NFKB p65 (Rel A) pS276 Anti-NFKB p65 (Rel A) pS276 (RABBIT) Antibody - 600-401-264 lot 24040 was probed against Normal (Lane 1) and TNFalpha (Lane 2) Stimulated HeLa whole cell lysates. A band was observed between 55 and 72 kD corresponding to the expected MW of NFkB p65. The observed higher MW bands have not been characterized.
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RELA / NFKB p65 Rabbit anti-Human Polyclonal (pSer276) Antibody
Human
ELISA, IHC, IP, WB
Unconjugated
100 µg/$498
RELA / NFKB p65 ELISA Kit
Select
Sandwich
96-Well Microplate
Rat
78.1 - 5000 pg/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$693
RELA / NFKB p65 Antibody - Immunohistochemical analysis of NF-kappaB p65 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Select
RELA / NFKB p65 Rabbit anti-Human Polyclonal (Internal) Antibody
Mouse, Bovine, Rat, Pig, Human, Monkey
IHC, IHC-P, IP, WB
Unconjugated
100 µl/$289
RELA / NFKB p65 Antibody - Immunohistochemical analysis of NF-kappaB p65 (pS536) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Select
RELA / NFKB p65 Rabbit anti-Human Polyclonal (pSer536) Antibody
Mouse, Rat, Pig, Human, Monkey
IHC, IHC-P, IP, WB
Unconjugated
100 µl/$305
RELA / NFKB p65 Antibody - Hela cells stained with purified rabbit polyclonal antibody against NF-kappaB p65 (poly6226), followed by Rhodamine Red-X conjugated goat anti-rabbit IgG and DAPI
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RELA / NFKB p65 Rabbit anti-Mouse Polyclonal (C-Terminus) Antibody
Mouse, Rat, Human
ICC, WB
Unconjugated
50 µl/$266
Select
RELA / NFKB p65 Mouse anti-Human Monoclonal Antibody
Human
ELISA, Flo, GS, ICC, IF, IHC, IHC-Fr, IHC-P, WB
Unconjugated
100 µg/$733
RELA / NFKB p65 Antibody - Western blot of Mono-Methyl-NFkappaB-p65 (K314/K315) antibody
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RELA / NFKB p65 Rabbit anti-Human Polyclonal (mono-methylation site of K314/K315) Antibody
Mouse, Rat, Human
ELISA, WB
Unconjugated
50 µl/$279; 100 µl/$309; 200 µl/$379
RELA / NFKB p65 Antibody - 1:100 staining mouse kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Select
RELA / NFKB p65 Rabbit anti-Human Polyclonal (pSer536) Antibody
Mouse, Rat, Human
ELISA, IF, IHC, IP, WB
Unconjugated
100 µl/$369; 200 µl/$411
RELA / NFKB p65 Antibody - Immunohistochemical analysis of NF-kappaB p65 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Select
RELA / NFKB p65 Rabbit anti-Human Polyclonal (Internal) Antibody
Mouse, Bovine, Rat, Pig, Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100 µl/$289
RELA / NFKB p65 Antibody - 1:200 staining human kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Select
RELA / NFKB p65 Rabbit anti-Human Polyclonal (pThr254) Antibody
Mouse, Rat, Human
ELISA, IF, IHC, IP, WB
Unconjugated
100 µl/$369; 200 µl/$411
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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).