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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B653-50 50 µl (80 mg/ml) $485 
RELA / NFKB p65 Antibody - Anti-RELA antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
RELA / NFKB p65 Antibody - Anti-RELA antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
RELA / NFKB p65 Antibody - Anti-NFkb p 65 Rel A Antibody - Western Blot. Western blot of HeLa cell extract. A predominant band ~65 kD (arrowhead) corresponding to NFkb p65 Rel A is observed. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a 1:5000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5 each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2000 for 1 h. Washes with TBS preceded color development.
RELA / NFKB p65 Antibody - Immunoblot of HeLa cell extract using anti-NFkb p65 (RelA) at a 1:5000 dilution. A predominant band ~65 kDa (arrowhead) corresponding to NFkb p65 Rel A is observed
RELA / NFKB p65 Antibody - Anti-RELA antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
RELA / NFKB p65 Antibody - Anti-RELA antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
RELA / NFKB p65 Antibody - Anti-NFkb p 65 Rel A Antibody - Western Blot. Western blot of HeLa cell extract. A predominant band ~65 kD (arrowhead) corresponding to NFkb p65 Rel A is observed. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a 1:5000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5 each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2000 for 1 h. Washes with TBS preceded color development.
RELA / NFKB p65 Antibody - Immunoblot of HeLa cell extract using anti-NFkb p65 (RelA) at a 1:5000 dilution. A predominant band ~65 kDa (arrowhead) corresponding to NFkb p65 Rel A is observed
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IHC‑plus™ Polyclonal Rabbit anti‑Human RELA / NFKB p65 Antibody (C‑Terminus, IHC, WB) LS‑B653

IHC‑plus™ Polyclonal Rabbit anti‑Human RELA / NFKB p65 Antibody (C‑Terminus, IHC, WB) LS‑B653

Note: This antibody replaces LS-C18902
Antibody:
RELA / NFKB p65 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, WB, ELISA, ChrIP, GS
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B653-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
RELA / NFKB p65 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, WB, ELISA, ChrIP, GS
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
NFKB p65 antibody LS-B653 is an unconjugated rabbit polyclonal antibody to NFKB p65 (RELA) (C-Terminus) from human. It is reactive with human, mouse and rat. Validated for ChrIP, ELISA, GS, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 9 publications.
Target
Human RELA / NFKB p65
Synonyms
RELA | NF-kappa-B p65delta3 | NF-kappaB | NFKB3 | p65 | Transcription factor p65 | NF kappaB | NFKB
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Immunogen
NFkB p65 (Rel A) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Epitope
C-Terminus
Applications
  • IHC
  • IHC - Paraffin (1:500)
  • Western blot (1:2000 - 1:5000)
  • ELISA (1:5000)
  • Chromatin Immunoprecipitation
  • Gel shift
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B653 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B653 was determined to be 1:500.
Presentation
0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RELA / NFKB p65
Q04206 NM_021975 NP_068810.3

Validation

RELA / NFKB p65 Antibody - Anti-RELA antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-RELA antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-RELA antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

RELA / NFKB p65 Antibody - Anti-RELA antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-RELA antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-RELA antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

LSBio Ratings

IHC-plus™ RELA / NFKB p65 Antibody (C-Terminus) for IHC, WB/Western, ELISA LS-B653 has an LSBio Rating of
Publications (4.8)
Laboratory Validation Score (4)

Publications (9)

Dexamethasone attenuates NF-kappa B DNA binding activity without inducing I kappa B levels in rat brain in vivo. Unlap MT, Jope RS. Brain research. Molecular brain research. 1997 45:83-9.

Curcumin blocks cytokine-mediated NF-kappa B activation and proinflammatory gene expression by inhibiting inhibitory factor I-kappa B kinase activity. Jobin C, Bradham CA, Russo MP, Juma B, Narula AS, Brenner DA, Sartor RB. Journal of immunology (Baltimore, Md. : 1950). 1999 163:3474-83.

Upregulation of HMG1 leads to melanoma inhibitory activity expression in malignant melanoma cells and contributes to their malignancy phenotype. Poser I, Golob M, Buettner R, Bosserhoff AK. Molecular and cellular biology. 2003 23:2991-8.

NF-kappaB1 (p50) homodimers differentially regulate pro- and anti-inflammatory cytokines in macrophages. Cao S, Zhang X, Edwards JP, Mosser DM. The Journal of biological chemistry. 2006 281:26041-50.

Glutathione depletion down-regulates tumor necrosis factor alpha-induced NF-kappaB activity via IkappaB kinase-dependent and -independent mechanisms. Lou H, Kaplowitz N. The Journal of biological chemistry. 2007 282:29470-81.

CXCL5 promotes prostate cancer progression. Begley LA, Kasina S, Mehra R, Adsule S, Admon AJ, Lonigro RJ, Chinnaiyan AM, Macoska JA. Neoplasia (New York, N.Y.). 2008 10:244-54.

Protein kinase A-induced phosphorylation of the p65 subunit of nuclear factor-kappaB promotes Schwann cell differentiation into a myelinating phenotype. Yoon C, Korade Z, Carter BD. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2008 28:3738-46.

Axonal neuregulin 1 type III activates NF-kappaB in Schwann cells during myelin formation. Limpert AS, Carter BD. The Journal of biological chemistry. 2010 285:16614-22.

Nuclear factor-B contributes to neuron-dependent induction of glutamate transporter-1 expression in astrocytes. Ghosh M, Yang Y, Rothstein JD, Robinson MB. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2011 31:9159-69.

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Requested From: United States
Date Requested: 9/22/2020