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Catalog Number Size Price
LS-C18905-100 100 µg (1 mg/ml) $498 
RELA / NFKB p65 Antibody - Anti-p65 NLS Antibody - Immunohistochemistry. Rabbit anti-p65 NLS was used at a 1:200 dilution to detect p65 in (A) control DU145 cells and (B) TNF stimulated DU145 cells. Although DU145 show relatively high basal levels of nuclear p65 staining, significant enhancement of nuclear staining is seen in panel B as evidence of translocation and availability of the NLS to be bound by the antibody. Cultured cells shown above were formalin-fixed. tissue staining (not shown) were formalin-fixed, paraffin embedded followed by citrate retrieval. Blocking and hybridization included 5% NGS.
RELA / NFKB p65 Antibody - Anti-p65 NLS Antibody - Immunofluorescence Microscopy. Rabbit anti-p65 NLS was used at a 1:200 dilution to detect p65 in TNF stimulated DU145 cells. Image shown is at a 1:400 magnification. Tissue was fixed and prepared as above.
RELA / NFKB p65 Antibody - Anti-p65 NLS Antibody - Immunohistochemistry. Rabbit anti-p65 NLS was used at a 1:200 dilution to detect p65 in (A) control DU145 cells and (B) TNF stimulated DU145 cells. Although DU145 show relatively high basal levels of nuclear p65 staining, significant enhancement of nuclear staining is seen in panel B as evidence of translocation and availability of the NLS to be bound by the antibody. Cultured cells shown above were formalin-fixed. tissue staining (not shown) were formalin-fixed, paraffin embedded followed by citrate retrieval. Blocking and hybridization included 5% NGS.
RELA / NFKB p65 Antibody - Anti-p65 NLS Antibody - Immunofluorescence Microscopy. Rabbit anti-p65 NLS was used at a 1:200 dilution to detect p65 in TNF stimulated DU145 cells. Image shown is at a 1:400 magnification. Tissue was fixed and prepared as above.
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Polyclonal Rabbit anti‑Human RELA / NFKB p65 Antibody (IHC, IF, WB) LS‑C18905

Polyclonal Rabbit anti‑Human RELA / NFKB p65 Antibody (IHC, IF, WB) LS‑C18905

Antibody:
RELA / NFKB p65 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, ELISA, GS
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$498
LS-C18905-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
RELA / NFKB p65 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, ELISA, GS
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
NFKB p65 antibody LS-C18905 is an unconjugated rabbit polyclonal antibody to human NFKB p65 (RELA). Validated for ELISA, GS, IF, IHC and WB. Cited in 3 publications.
Target
Human RELA / NFKB p65
Synonyms
RELA | NF-kappa-B p65delta3 | NF-kappaB | NFKB3 | p65 | Transcription factor p65 | NF kappaB | NFKB
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This antibody was purified from whole rabbit serum prepared by repeated immunizations with the NFkB p65 peptide corresponding to the NLS of the human protein conjugated to KLH using maleimide.  A residue of cysteine was added to the amino terminal end to facilitate coupling.
Specificity
The antibody selectively binds to the activated form of NFkB. Anti-NFkB p65 NLS may react non-specifically with other proteins.
Applications
  • IHC
  • IHC - Paraffin (1:200)
  • Immunofluorescence (1:200)
  • Western blot (1:2000)
  • ELISA (1:5000 - 1:25000)
  • Gel shift
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for immunohistochemistry, immunoblotting, ELISA and supershift assays. This product was assayed by immunoblot and found to be reactive against NFxB p65 NLS at a dilution of 1:2000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) catalog no. LS-C60884. Anti-NFxB NLS is suitable for the gel supershift assay and was found to be reactive against all p65 (Rel A) containing human NFxB complexes using 0.5 to 1.0 ul per assay.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RELA / NFKB p65
Q04206 NM_021975 NP_068810.3

LSBio Ratings

RELA / NFKB p65 Antibody for IHC, IF/Immunofluorescence, WB/Western, ELISA LS-C18905 has an LSBio Rating of
Publications (4.2)

Publications (3)

Diabetes-induced activation of canonical and noncanonical nuclear factor-kappaB pathways in renal cortex. Starkey JM, Haidacher SJ, LeJeune WS, Zhang X, Tieu BC, Choudhary S, Brasier AR, Denner LA, Tilton RG. Diabetes. 2006 55:1252-9.

Antiinflammatory adaptation to hypoxia through adenosine-mediated cullin-1 deneddylation. Khoury J, Ibla JC, Neish AS, Colgan SP. The Journal of clinical investigation. 2007 117:703-11.

Adenosine signaling mediates SUMO-1 modification of IkappaBalpha during hypoxia and reoxygenation. Liu Q, Li J, Khoury J, Colgan SP, Ibla JC. The Journal of biological chemistry. 2009 284:13686-95.

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/14/2022